CXCR4 Antibody (44717) [Unconjugated]

Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB173). The ND50 is typically 1-5 µg/mL.

Ectopic expression of CXCR7 and CXCR4 on MDCK cells.(A) MDCK were stably transfected with empty vector (Mock), with CXCR7, CXCR4, a vector coding for a CXCR7 lacking the cytoplasmic C-terminus ( delta CXCR7), and a vector coding for a chimeric CXCR7 containing the DRYLAIV motive of CXCR4. Receptor expression was determined by FACS analysis using saturating antibody concentrations (see Methods). (B) Confocal immunofluorescence analysis of unfixed MDCK cells expressing CXCR7 (upper panels) or CXCR4 (lower panels). Cells also expressed N-ter-Lck mCherry as membrane marker (red fluorescence). Left panels: confocal images of planes cut through intracellular regions of MDCK monolayers. Right panels: x-z planes reconstructed from confocal x-y stacks. For receptor (green) detection anti-CXCR7 (11G8 R&D) or anti CXCR4 (MAB173 R&D) were used. Receptor-bound primary antibodies were revealed with goat anti mouse IgG conjugated with Alexa488 (green fluorescence). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20161793), licensed under a CC-BY license. Not internally tested by R&D Systems.

In vitro cell invasion is stimulated by the SDF-1/CXCR4 pathway independently from HPV status.The modulation of E6 expression was monitored using Western-blot in HPV-positive HeLa (A) and TC-1 (B) cells and in HPV-negative B16F10 (C) cells, after 3 and 6 days of incubation with Cidofovir (CDF). Modulation of P53 expression was assessed in HeLa cells after CDF incubation (A). Cell invasion was measured using a Matrigel assay in HeLa (A), TC-1 (B) and B16F10 (C) cells. Recombinant human CXCL12/SDF-1 (100 ng/mL; R&D Systems) was used as chemoattractant and modulation of cell migration was recorded after treatment with CXCR4-blocking antibody or/and Cidofovir (CDF). The invasion rate was determined by counting crystal violet-stained cells. Invasion was stimulated by SDF-1 alpha /CXCR4 independently from the HPV status of the cells but Cidofovir anti-invasive action was restricted to the two HPV-positive cell lines. Three independent experiments with three chambers each time were performed. ***P<0.01, for a statistically true difference, as compared to the untreated group. # P<0.01 compared to SDF-1 alpha -treated group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/19325708), licensed under a CC-BY license. Not internally tested by R&D Systems.

TC-1 cell lung metastasis is mediated by the CXCR4 in vivo.TC-1 cells (1×106) were injected into mice tail veins either without or with pre-treatment with CXCR4-blocking antibody (2B11), irrelevant antibody (CD71), or trypsin/EDTA. (A). Representative Hematoxylin-Eosin-Saffranin (HES)-satined sections of lungs of mice are shown. Digitization of the whole slides was performed for each animal/treatment type. Histopathological analysis was done after delineation of metastases (dashed line) vs whole lung area (B) on each HES-stained section with the ImageJ software. The lung metastasis indices within each cohort of mice are expressed as fold decrease (+/−SEM) over untreated controls ***P<0.01. (C) Representative sections of CXCR4 immunostaining are shown. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/19325708), licensed under a CC-BY license. Not internally tested by R&D Systems.

In vitro cell invasion is stimulated by the SDF-1/CXCR4 pathway independently from HPV status.The modulation of E6 expression was monitored using Western-blot in HPV-positive HeLa (A) and TC-1 (B) cells and in HPV-negative B16F10 (C) cells, after 3 and 6 days of incubation with Cidofovir (CDF). Modulation of P53 expression was assessed in HeLa cells after CDF incubation (A). Cell invasion was measured using a Matrigel assay in HeLa (A), TC-1 (B) and B16F10 (C) cells. Recombinant human CXCL12/SDF-1 (100 ng/mL; R&D Systems) was used as chemoattractant and modulation of cell migration was recorded after treatment with CXCR4-blocking antibody or/and Cidofovir (CDF). The invasion rate was determined by counting crystal violet-stained cells. Invasion was stimulated by SDF-1 alpha /CXCR4 independently from the HPV status of the cells but Cidofovir anti-invasive action was restricted to the two HPV-positive cell lines. Three independent experiments with three chambers each time were performed. ***P<0.01, for a statistically true difference, as compared to the untreated group. # P<0.01 compared to SDF-1 alpha -treated group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/19325708), licensed under a CC-BY license. Not internally tested by R&D Systems.

TC-1 cell lung metastasis is mediated by the CXCR4 in vivo.TC-1 cells (1×106) were injected into mice tail veins either without or with pre-treatment with CXCR4-blocking antibody (2B11), irrelevant antibody (CD71), or trypsin/EDTA. (A). Representative Hematoxylin-Eosin-Saffranin (HES)-satined sections of lungs of mice are shown. Digitization of the whole slides was performed for each animal/treatment type. Histopathological analysis was done after delineation of metastases (dashed line) vs whole lung area (B) on each HES-stained section with the ImageJ software. The lung metastasis indices within each cohort of mice are expressed as fold decrease (+/−SEM) over untreated controls ***P<0.01. (C) Representative sections of CXCR4 immunostaining are shown. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/19325708), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow, B/N, CyTOF-ready
• Clone: 44717
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCR4 Antibody (44717) [Unconjugated] Summary

• Immunogen: 3T3 cells transfected with human CXCR4
• Specificity: Reacts specifically with human and non-human cells expressing human CXCR4 (fusin) as detected by flow cytometry. It will also react with cells expressing feline CXCR4 but not rat CXCR4. This antibody does not cross-react with other chemokine receptors.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCR4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 1-5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR4 Antibody (44717) [Unconjugated]

• CD184
• chemokine (C-X-C motif) receptor 4
• chemokine (C-X-C motif), receptor 4 (fusin)
• C-X-C chemokine receptor type 4
• CXCR4
• CXC-R4
• CXCR-4
• D2S201E
• D2S201ESDF-1 receptor
• FB22
• Fusin
• HM89
• HM89NPYRL
• HSY3RR
• LAP3
• LAP-3
• LCR1
• LESTR
• LESTRCD184 antigen
• Leukocyte-derived seven transmembrane domain receptor
• leukocyte-derived seven-transmembrane-domain receptor
• lipopolysaccharide-associated protein 3
• neuropeptide Y receptor Y3
• NPY3R
• NPY3RWHIM
• NPYR
• NPYRL
• NPYY3R
• pCXCR4
• seven transmembrane helix receptor
• seven-transmembrane-segment receptor, spleen
• Stromal cell-derived factor 1 receptor
• WHIMS

Background

CXCR4, also known as CD184, is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells. While primarily a membrane protein, CXCR4 undergoes trafficking and internalization in response to stimulation with phorbol esters and ligand (1). Cytoplasmic and nuclear localization of CXCR4 has been observed in colorectal and renal carcinomas (2,3) and it has been used as the basis of prognosis and metastatic state (3,4,5).

1. Orsini, M.J. et al. (1999) J. Biol. Chem. 274:31076.
2. Zagzag, D. et al. (2005) Cancer Res. 65:6178.
3. Speetjens, F.M. et al. (2009) Cancer Microenvironment 2:1.
4. Wang, L. et al. (2009) Oncology Reports 22:1333.
5. Amara, S. et al. (2015) Cancer Biomark. 15:869.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR4 Antibody (MAB173)(31)

Publications using MAB173

• Awad, M;Taylor-Diaz, E;Tawfik, A;Hussein, K;Elmansi, A;Elashiry, M;Elsayed, R;Shahoumi, L;Borke, J;Hill, W;Dong, F;Elsalanty, ME; Zoledronate interrupts pre-osteoclast-induced angiogenesis via SDF-1/CXCR4 pathway Bone reports 2024-12-01 [PMID: 39583183] (Western Blot, Human)
• García-Cuesta, EM;Martínez, P;Selvaraju, K;Ulltjärn, G;Gómez Pozo, AM;D'Agostino, G;Gardeta, S;Quijada-Freire, A;Blanco Gabella, P;Roca, C;Hoyo, DD;Jiménez-Saiz, R;García-Rubia, A;Soler Palacios, B;Lucas, P;Ayala-Bueno, R;Santander Acerete, N;Carrasco, Y;Oscar Sorzano, C;Martinez, A;Campillo, NE;Jensen, LD;Rodriguez Frade, JM;Santiago, C;Mellado, M; Allosteric modulation of the CXCR4:CXCL12 axis by targeting receptor nanoclustering via the TMV-TMVI domain eLife 2024-09-09 [PMID: 39248648] (Flow Cytometry, Human)
• Tundidor, I;Seijo-Vila, M;Blasco-Benito, S;Rubert-Hern�ndez, M;Ad�mez, S;Andradas, C;Manzano, S;�lvarez-L�pez, I;Sarasqueta, C;Villa-Morales, M;Gonz�lez-Lois, C;Ram�rez-Medina, E;Almoguera, B;S�nchez-L�pez, AJ;Bindila, L;Hamann, S;Arnold, N;R�cken, C;Heras-Murillo, I;Sancho, D;Moreno-Bueno, G;Caffarel, MM;Guzm�n, M;S�nchez, C;P�rez-G�mez, E; Identification of fatty acid amide hydrolase as a metastasis suppressor in breast cancer Nature communications 2023-05-30 [PMID: 37253733] (Flow Cytometry, Human)
• Y Shamshirga, A Jonebring, A Svensson, I Leefa, M Bohlooly-Y, M Firth, KJ Woollard, A Hofherr, IM Rogers, R Hicks Rapid target validation in a Cas9-inducible hiPSC derived kidney model Scientific Reports, 2021-08-16;11(1):16532. 2021-08-16 [PMID: 34400685] (IHC, Human)
• M Armani-Tou, Z Zhou, R Gasser, I Staropoli, V Cantaloube, Y Benureau, J Garcia-Per, M Pérez-Olme, V Lorin, B Puissant-L, L Assoumou, C Delaugerre, JD Lelièvre, Y Lévy, H Mouquet, G Martin-Blo, J Alcami, F Arenzana-S, J Izopet, P Colin, B Lagane Mechanisms of HIV-1 evasion to the antiviral activity of chemokine CXCL12 indicate potential links with pathogenesis PloS Pathogens, 2021-04-19;17(4):e1009526. 2021-04-19 [PMID: 33872329] (Binding Assay, Human)
• PA Subramani, N Vartak-Sha, S Sreekumar, P Mathur, B Nayer, S Dakhore, SK Basavanna, DM Kalappa, RV Krishnamur, B Mukhi, P Mishra, N Yoshida, SK Ghosh, R Shandil, S Narayanan, B Campo, K Hasegawa, AR Anvikar, N Valecha, V Sundaramur Plasmodium vivax liver stage assay platforms using Indian clinical isolates Malar. J., 2020-06-22;19(1):214. 2020-06-22 [PMID: 32571333] (ICC, Human)
• F Facciotti, P Larghi, R Bosotti, C Vasco, N Gagliani, C Cordiglier, S Mazzara, V Ranzani, E Rottoli, S Curti, A Penatti, B Karnani, Y Kobayashi, M Crosti, M Bombaci, JP van Hambur, G Rossetti, R Gualtierot, M Gerosa, S Gatti, S Torretta, L Pignataro, SW Tas, S Abrignani, M Pagani, F Grassi, PL Meroni, RA Flavell, J Geginat Evidence for a pathogenic role of extrafollicular, IL-10-producing CCR6+B helper T cells in systemic lupus erythematosus Proc. Natl. Acad. Sci. U.S.A., 2020-03-17;117(13):7305-7316. 2020-03-17 [PMID: 32184325] (Flow Cytometry, Human)
• Mohaned Ahmed, Haneen A. Basheer, Jose M. Ayuso, Djevdet Ahmet, Marco Mazzini, Roshan Patel et al. Agarose Spot as a Comparative Method for in situ Analysis of Simultaneous Chemotactic Responses to Multiple Chemokines Scientific Reports 2017-04-21 [PMID: 28432337]
• R Desplantes, C Lévêque, B Muller, M Lotierzo, G Ferracci, M Popoff, M Seagar, R Mamoun, O El Far Affinity biosensors using recombinant native membrane proteins displayed on exosomes: application to botulinum neurotoxin B receptor Sci Rep, 2017-04-21;7(1):1032. 2017-04-21 [PMID: 28432329] (Surface Plasmon Resonance)
• Pengcui Li, Jin Deng, Xiaochun Wei, Chathuraka T. Jayasuriya, Jingming Zhou, Qian Chen et al. Blockade of hypoxia-induced CXCR4 with AMD3100 inhibits production of OA-associated catabolic mediators IL-1 beta and MMP-13 Molecular Medicine Reports 2016-08-01 [PMID: 27356492] (Immunocytochemistry, Human)
• Huang , Keven, Kiefer , Christin, Kamal , Adeela Novel role for NFAT3 in ERK-mediated regulation of CXCR4. PLoS ONE, 2014-12-16;9(12):e115249. 2014-12-16 [PMID: 25514788] (Western Blot, Human)
• Carrega , Paolo, Bonaccorsi , Irene, Di Carlo , Emma, Morandi , Barbara, Paul , Petra, Rizzello , Valeria, Cipollone , Giuseppe, Navarra , Giuseppe, Mingari , Maria Cr, Moretta , Lorenzo, Ferlazzo , Guido CD56(bright)perforin(low) noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph. J Immunol, 2014-03-19;192(8):3805-15. 2014-03-19 [PMID: 24646734] (Flow Cytometry, Human)
• Yin X, Johns S, Kim D, Mikulski Z, Salanga C, Handel T, Macal M, Zuniga E, Fuster M Lymphatic specific disruption in the fine structure of heparan sulfate inhibits dendritic cell traffic and functional T cell responses in the lymph node. J Immunol, 2014-02-03;192(5):2133-42. 2014-02-03 [PMID: 24493818] (Blocking, Human)
• Hannan , Nicholas, Segeritz , Charis-P, Touboul , Thomas, Vallier , Ludovic Production of hepatocyte-like cells from human pluripotent stem cells. Nat Protoc, 2013-01-01;8(2):430-7. 2013-01-01 [PMID: 23370178] (ICC, Human)
• Duluc , D, Gannevat , J, Anguiano , E, Zurawski , S, Carley , M, Boreham , M, Stecher , J, Dullaers , M, Banchereau , J, Oh , S Functional diversity of human vaginal APC subsets in directing T-cell responses. Mucosal Immunol, 2012-11-07;6(3):626-38. 2012-11-07 [PMID: 23131784] (Flow Cytometry, Human)
• Kulbe H, Chakravarty P, Leinster DA, Charles KA, Kwong J, Thompson RG, Coward JI, Schioppa T, Robinson SC, Gallagher WM, Galletta L, Salako MA, Smyth JF, Hagemann T, Brennan DJ, Bowtell DD, Balkwill FR A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment. Cancer Res., 2011-11-07;72(1):66-75. 2011-11-07 [PMID: 22065722] (IHC-P, Human)
• M Fareh, L Turchi, V Virolle, D Debruyne, F Almairac, S de-la-Forest Divonne et al. The miR 302-367 cluster drastically affects self-renewal and infiltration properties of glioma-initiating cells through CXCR4 repression and consequent disruption of the SHH-GLI-NANOG network Cell Death & Differentiation 2012-02-01 [PMID: 21720384] (Immunocytochemistry, Human)
• Naumann U, Cameroni E, Pruenster M, Mahabaleshwar H, Raz E, Zerwes HG, Rot A, Thelen M CXCR7 functions as a scavenger for CXCL12 and CXCL11. PLoS ONE, 2010-02-11;5(2):e9175. 2010-02-11 [PMID: 20161793] (ICC, Human)
• Rahimi Massod, George Jessica, Tang Careen et al. EGFR variant-mediated invasion by enhanced CXCR4 expression through transcriptional and post-translational mechanisms. International Journal of Cancer 1/1/2010 [PMID: 19830694]
• Kollmar O, Rupertus K, Scheuer C, Nickels RM, Haberl GC, Tilton B, Menger MD, Schilling MK CXCR4 and CXCR7 regulate angiogenesis and CT26.WT tumor growth independent from SDF-1. Int. J. Cancer, 2010-03-15;126(6):1302-15. 2010-03-15 [PMID: 19821487] (Neutralization, Mouse)
• Albanesi C, Scarponi C, Pallotta S, Daniele R, Bosisio D, Madonna S, Fortugno P, Gonzalvo-Feo S, Franssen JD, Parmentier M, De Pita O, Girolomoni G, Sozzani S Chemerin expression marks early psoriatic skin lesions and correlates with plasmacytoid dendritic cell recruitment. J. Exp. Med., 2008-12-29;206(1):249-58. 2008-12-29 [PMID: 19114666] (Blocking, Human)
• Hansson M, Hermansson M, Svensson H, Elfvin A, Hansson LE, Johnsson E, Sjoling A, Quiding-Jarbrink M CCL28 is increased in human Helicobacter pylori-induced gastritis and mediates recruitment of gastric immunoglobulin A-secreting cells. Infect. Immun., 2008-04-21;76(7):3304-11. 2008-04-21 [PMID: 18426876] (Flow Cytometry, Human)
• Struckmann K, Mertz K, Steu S, Storz M, Staller P, Krek W, Schraml P, Moch H pVHL co-ordinately regulates CXCR4/CXCL12 and MMP2/MMP9 expression in human clear-cell renal cell carcinoma. J. Pathol., 2008-03-01;214(4):464-71. 2008-03-01 [PMID: 18189328] (ICC, Human)
• Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells. J. Immunol., 2007-09-15;179(6):3724-33. 2007-09-15 [PMID: 17785809] (Flow Cytometry, Human)
• Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities. Stem Cells, 2007-03-29;25(7):1737-45. 2007-03-29 [PMID: 17395768] (Flow Cytometry, Human)
• Lim HW, Hillsamer P, Kim CH Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses. J. Clin. Invest., 2004-12-01;114(11):1640-9. 2004-12-01 [PMID: 15578096] (ICC, Human)
• Curiel , Tyler J, Coukos , George, Zou , Linhua, Alvarez , Xavier, Cheng , Pui, Mottram , Peter, Evdemon-Hogan , Melina, Conejo-Garcia , Jose R, Zhang , Lin, Burow , Matthew, Zhu , Yun, Wei , Shuang, Kryczek , Ilona, Daniel , Ben, Gordon , Alan, Myers , Leann, Lackner , Andrew, Disis , Mary L, Knutson , Keith L, Chen , Lieping, Zou , Weiping Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced survival. Nat Med, 2004-08-22;10(9):942-9. 2004-08-22 [PMID: 15322536] (Neutralization, Human)
• de Parseval A, Ngo S, Sun P, Elder JH Factors that increase the effective concentration of CXCR4 dictate feline immunodeficiency virus tropism and kinetics of replication. J. Virol., 2004-09-01;78(17):9132-43. 2004-09-01 [PMID: 15308709] (Flow Cytometry, Feline)
• Hatse S, Princen K, Vermeire K, Gerlach LO, Rosenkilde MM, Schwartz TW, Bridger G, De Clercq E, Schols D Mutations at the CXCR4 interaction sites for AMD3100 influence anti-CXCR4 antibody binding and HIV-1 entry. FEBS Lett., 2003-07-10;546(2):300-6. 2003-07-10 [PMID: 12832058] (Flow Cytometry, Human)
• Willett BJ, Cannon CA, Hosie MJ Expression of CXCR4 on feline peripheral blood mononuclear cells: effect of feline immunodeficiency virus infection. J. Virol., 2003-01-01;77(1):709-12. 2003-01-01 [PMID: 12477874] (Flow Cytometry, Feline)
• Nguyen DH, Taub D CXCR4 function requires membrane cholesterol: implications for HIV infection. J. Immunol., 2002-04-15;168(8):4121-6. 2002-04-15 [PMID: 11937572] (Flow Cytometry, Neutralization, Human)

FAQs for CXCR4 Antibody (MAB173). (Showing 1 - 1 of 1 FAQs).

1. Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.
   • CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

Bioinformatics

• Gene Symbol: CXCR4