CXCR4 Antibody (44717) [Phycoerythrin]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CXCR4 PE-conjugated Monoclonal Antibody (Catalog # FAB173P) and Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (FAB4867A) for 15 minutes at 37°C followed by 15 minutes at room temperature. View our protocol for Staining Membrane-associated Proteins.

Representative example of flow cytometry quantification of complexes neutrophil–CD41a+ (NPAs, neutrophil–platelet aggregates) and lymphocyte markers’ expression. (A) Neutrophil cluster identification (region R1) based on its low CD14 expression (FL3) and side-scattering (SSC) morphological characteristics. (B) The R1-based histogram’s subtraction analysis (positive events (continuous line) minus isotype control (dotted line)) was used to quantify both the percentage of complexes CD41a+ (grey events in M1) and their RFI (median of M1 histogram minus median of the isotype control). (C) Lymphocyte cluster identification based on its forward- (FSC) and side-scattering characteristics (region R1). (D) Representative quantification of lymphocyte CXCR4 expression following the overlay histogram’s subtraction analysis (see above). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36836785), licensed under a CC-BY license. Not internally tested by R&D Systems.

Representative example of flow cytometry quantification of complexes neutrophil–CD41a+ (NPAs, neutrophil–platelet aggregates) and lymphocyte markers’ expression. (A) Neutrophil cluster identification (region R1) based on its low CD14 expression (FL3) and side-scattering (SSC) morphological characteristics. (B) The R1-based histogram’s subtraction analysis (positive events (continuous line) minus isotype control (dotted line)) was used to quantify both the percentage of complexes CD41a+ (grey events in M1) and their RFI (median of M1 histogram minus median of the isotype control). (C) Lymphocyte cluster identification based on its forward- (FSC) and side-scattering characteristics (region R1). (D) Representative quantification of lymphocyte CXCR4 expression following the overlay histogram’s subtraction analysis (see above). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36836785), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow
• Clone: 44717
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

CXCR4 Antibody (44717) [Phycoerythrin] Summary

• Immunogen: Mouse 3T3 cells transfected with human CXCR4
• Specificity: Reacts specifically with human and non-human cells expressing human CXCR4 (fusin) as detected by flow cytometry. It will also react with cells expressing feline CXCR4 but not rat CXCR4. This antibody does not cross-react with other chemokine receptors.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCR4
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR4 Antibody (44717) [Phycoerythrin]

• CD184
• chemokine (C-X-C motif) receptor 4
• chemokine (C-X-C motif), receptor 4 (fusin)
• C-X-C chemokine receptor type 4
• CXCR4
• CXC-R4
• CXCR-4
• D2S201E
• D2S201ESDF-1 receptor
• FB22
• Fusin
• HM89
• HM89NPYRL
• HSY3RR
• LAP3
• LAP-3
• LCR1
• LESTR
• LESTRCD184 antigen
• Leukocyte-derived seven transmembrane domain receptor
• leukocyte-derived seven-transmembrane-domain receptor
• lipopolysaccharide-associated protein 3
• neuropeptide Y receptor Y3
• NPY3R
• NPY3RWHIM
• NPYR
• NPYRL
• NPYY3R
• pCXCR4
• seven transmembrane helix receptor
• seven-transmembrane-segment receptor, spleen
• Stromal cell-derived factor 1 receptor
• WHIMS

Background

CXCR4 is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR4 Antibody (FAB173P)(16)

Publications using FAB173P

• Katia K. Mattis, Nicole A. J. Krentz, Christoph Metzendorf, Fernando Abaitua, Aliya F. Spigelman, Han Sun et al. Loss of RREB1 in pancreatic beta cells reduces cellular insulin content and affects endocrine cell gene expression Diabetologia 2023-04-01 [PMID: 36633628] (Flow Cytometry, Human)
• Katia K. Mattis, Nicole A. J. Krentz, Christoph Metzendorf, Fernando Abaitua, Aliya F. Spigelman, Han Sun et al. Loss of RREB1 in pancreatic beta cells reduces cellular insulin content and affects endocrine cell gene expression Diabetologia 4/1/2023 [PMID: 36633628]
• L Sadeghi, G Arvidsson, M Merrien, A M Wasik, A Görgens, CIE Smith, B Sander, AP Wright Differential B-Cell Receptor Signaling Requirement for Adhesion of Mantle Cell Lymphoma Cells to Stromal Cells Cancers (Basel), 2020-05-02;12(5):. 2020-05-02 [PMID: 32370190] (Flow Cytometry, Human)
• T Schober, T Magg, M Laschinger, M Rohlfs, ND Linhares, J Puchalka, T Weisser, K Fehlner, J Mautner, C Walz, K Hussein, G Jaeger, B Kammer, I Schmid, M Bahia, SD Pena, U Behrends, BH Belohradsk, C Klein, F Hauck A human immunodeficiency syndrome caused by mutations in CARMIL2 Nat Commun, 2017-01-23;8(0):14209. 2017-01-23 [PMID: 28112205] (Flow Cytometry, Human)
• Sheng-Bin Peng, Xiaoyi Zhang, Donald Paul, Lisa M. Kays, Ming Ye, Peter Vaillancourt et al. Inhibition of CXCR4 by LY2624587, a Fully Humanized Anti-CXCR4 Antibody Induces Apoptosis of Hematologic Malignancies PLOS ONE 3/8/2016 [PMID: 26954567]
• Buckner , Clarisa, Moir , Susan, Kardava , Lela, Ho , Jason, Santich , Brian H, Kim , Leo Jin, Funk , Emily K, Nelson , Amy K, Winckler , Britanny, Chairez , Cheryl L, Theobald-Whiting , Narda L, Anaya-O'Brien , Sandra, Alimchandani , Meghna, Quezado , Martha M, Yao , Michael, Kovacs , Joseph A, Chun , Tae-Wook, Fauci , Anthony, Malech , Harry L, De Ravin , Suk See CXCR4/IgG-expressing plasma cells are associated with human gastrointestinal tissue inflammation. J Allergy Clin Immunol, 2013-12-25;133(6):1676-85.e5. 2013-12-25 [PMID: 24373354] (Flow Cytometry, Human)
• Liu , Che, Pham , Kien, Luo , Defang, Reynolds , Brent A, Hothi , Parvinde, Foltz , Gregory, Harrison , Jeffrey Expression and functional heterogeneity of chemokine receptors CXCR4 and CXCR7 in primary patient-derived glioblastoma cells. PLoS ONE, 2013-03-21;8(3):e59750. 2013-03-21 [PMID: 23555768] (Flow Cytometry, Human)
• Erin F. Gillespie, Nupur Raychaudhuri, Konstantinos I. Papageorgiou, Stephen J. Atkins, Ying Lu, Laya K. Charara et al. Interleukin-6 Production in CD40-Engaged Fibrocytes in Thyroid-Associated Ophthalmopathy: Involvement of Akt and NF-kappa B Investigative Opthalmology & Visual Science 11/21/2012 [PMID: 23092922]
• Erin F. Gillespie, Nupur Raychaudhuri, Konstantinos I. Papageorgiou, Stephen J. Atkins, Ying Lu, Laya K. Charara et al. Interleukin-6 Production in CD40-Engaged Fibrocytes in Thyroid-Associated Ophthalmopathy: Involvement of Akt and NF-kappa B Investigative Opthalmology & Visual Science 2012-11-21 [PMID: 23092922] (Flow Cytometry, Human)
• Badr G, Lefevre EA, Mohany M Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis. PLoS ONE, 2011-09-01;6(9):e23741. 2011-09-01 [PMID: 21912642] (Flow Cytometry, Human)
• Raposo RA, Trudgian DC, Thomas B, van Wilgenburg B, Cowley SA, James W Protein kinase C and NF-kappaB-dependent CD4 downregulation in macrophages induced by T cell-derived soluble factors: consequences for HIV-1 infection. J. Immunol., 2011-06-10;187(2):748-59. 2011-06-10 [PMID: 21666058] (Flow Cytometry, Human)
• Harjeet Singh, Matthew J. Figliola, Margaret J. Dawson, Helen Huls, Simon Olivares, Kirsten Switzer et al. Reprogramming CD19-Specific T Cells with IL-21 Signaling Can Improve Adoptive Immunotherapy of B-Lineage Malignancies Cancer Research 5/15/2011 [PMID: 21558388]
• Scala S, Giuliano P, Ascierto PA, Ierano C, Franco R, Napolitano M, Ottaiano A, Lombardi ML, Luongo M, Simeone E, Castiglia D, Mauro F, De Michele I, Calemma R, Botti G, Caraco C, Nicoletti G, Satriano RA, Castello G Human melanoma metastases express functional CXCR4. Clin. Cancer Res., 2006-04-15;12(8):2427-33. 2006-04-15 [PMID: 16638848] (Flow Cytometry, Human)
• Hatse S, Princen K, Vermeire K, Gerlach LO, Rosenkilde MM, Schwartz TW, Bridger G, De Clercq E, Schols D Mutations at the CXCR4 interaction sites for AMD3100 influence anti-CXCR4 antibody binding and HIV-1 entry. FEBS Lett., 2003-07-10;546(2):300-6. 2003-07-10 [PMID: 12832058] (Flow Cytometry, Human)
• Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS. J. Leukoc. Biol., 2003-05-01;73(5):584-90. 2003-05-01 [PMID: 12714572] (Flow Cytometry, Human)
• de Lavareille A, Roufosse F, Schandene L, Stordeur P, Cogan E, Goldman M Clonal Th2 cells associated with chronic hypereosinophilia: TARC-induced CCR4 down-regulation in vivo. Eur. J. Immunol., 2001-04-01;31(4):1037-46. 2001-04-01 [PMID: 11298328] (Flow Cytometry, Human)

FAQs for CXCR4 Antibody (FAB173P). (Showing 1 - 1 of 1 FAQs).

1. Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.
   • CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

Bioinformatics

• Gene Symbol: CXCR4