Recombinant Mouse CXCL9/MIG Protein

SDS-Page: Mouse CXCL9/MIG Protein [NBP2-35191]

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse CXCL9/MIG Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 105 amino acids corresponding to CXCL9/MIG Source: E. coli

  Uniprot ID: P18340

  Amino Acid Sequence: TLVIRNARCS CISTSRGTIH YKSLKDLKQF APSPNCNKTE IIATLKNGDQ TCLDPDSANV KKLMKEWEKK INQKKKQKRG KKHQKNMKNR KPKTPQSRRR SRKTT

• Details of Functionality: MIG Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 0.1-1.0 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CXCL9
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of CXCL9/MIG as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 12.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse CXCL9/MIG Protein

• chemokine (C-X-C motif) ligand 9
• CMK
• crg-10
• C-X-C motif chemokine 9
• CXCL9
• Gamma-interferon-induced monokine
• Humig
• MIG
• MIG/CXCL9
• MIGSmall-inducible cytokine B9
• monokine induced by gamma interferon
• SCYB9Monokine induced by interferon-gamma

Background

CXCL9 belongs to the CXC chemokine family and also known as Monokine induced by gamma interferon (MIG). It is a T-cell chemoattractant induced by IFN-gamma. CXCL9 is closely related to two other CXC chemokines called CXCL10 and CXCL11, additionally they all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. It is a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response and chemotactic for activated T-cells. Recombinant murine CXCL9 contains 105 amino acids which is a single non-glycosylated polypeptide chain. Furthermore, The murine CXCL9 shares 75 % and 88 % a.a. sequence identity with human and rat CXCL9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for CXCL9/MIG Protein (NBP2-35191)(1)

Publication using NBP2-35191

• Bin Huang, Wenhao Wang, Qingchu Li et al. Osteoblasts secrete Cxcl9 to regulate angiogenesis in bone. Nat Commun 2016-12-14 [PMID: 27966526]

Bioinformatics

• Gene Symbol: CXCL9
• Uniprot:
  • P18340()
  • P18340()
  • P18340()