![Western Blot: CXCL2/GRO beta/MIP-2/CINC-3 Antibody [NBP3-03233] - Analysis of extracts of rat lung, using CxCL2/GRO beta/MIP-2/CINC-3 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize2/CXCL2-GRO-beta-MIP-2-CINC-3-Antibody-Western-Blot-NBP3-03233-img0001.jpg "Western Blot: CXCL2/GRO beta/MIP-2/CINC-3 Antibody [NBP3-03233] - Analysis of extracts of rat lung, using CxCL2/GRO beta/MIP-2/CINC-3 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
| Reactivity | RtSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-107 of human CXCL2/GRO beta/MIP-2/CINC-3 (NP_002080.1). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CXCL2 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 11 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS with 50% glycerol, pH7.3. |
| Preservative | 0.01% Thimerosal |
| Purity | Affinity purified |
![Western Blot: Transportin 1 Antibody (D45) [NB600-1397] - Human lung cancer cell line usig lysates from HCC44 cell line. (N: Nuclear fraction, C: Cytoplasmic fraction). Image from verified customer review.](http://images.novusbio.com/images2/Transportin-1-Antibody-(D45)-Western-Blot-NB600-1397-img0005.jpg)
![Western Blot: Transportin 1 Antibody (D45) [NB600-1397] - Whole cell protein from A431, Hek293 and MCF7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Transportin 1 (D45) in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/Transportin-1-Antibody-(D45)-Western-Blot-NB600-1397-img0004.jpg)
![Immunohistochemistry-Paraffin: Transportin 1 Antibody (D45) [NB600-1397] - IHC analysis of a formalin fixed paraffin embedded tissue section of human pancreas using Transportin 1 antibody (clone D45) at 1:200 dilution. The antibody generated specific nuclear and cytoplasmic signal of Transportin 1 protein in the glandular cells while the connective tissue was largely negative.](http://images.novusbio.com/images2/Transportin-1-Antibody-(D45)-Immunohistochemistry-Paraffin-NB600-1397-img0001.jpg)
![Dual RNAscope ISH-IHC: TLR4 Antibody (76B357.1) [NB100-56566] - FFPE tissue sections of human tonsil were probed for TLR4 mRNA (ACD RNAScope Probe, ACD catalog # 311281; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Mouse Monoclonal (Novus Biologicals catalog # NB100-56566) at 5ug/mL with 1 hour incubation at room temperature followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.](http://images.novusbio.com/images2/TLR4-Antibody-(76B357.1)-Dual-RNAscope-ISH-IHC-NB100-56566-img0036.jpg)
![Immunohistochemistry: TLR4 Antibody (76B357.1) [NB100-56566] - Immunofluorescent staining of TMAs. Representative tissue cores from normal (I), adenomatous polyps (II), and CRC (III and IV) are shown. Image collected and cropped by CiteAb from the following publication (http://www.jeccr.com/content/33/1/45), licensed under a CC-BY licence.](http://images.novusbio.com/images2/TLR4-Antibody-(76B357.1)-Immunohistochemistry-NB100-56566-img0037.jpg)
![Immunohistochemistry: TLR4 Antibody (76B357.1) [NB100-56566] - Pericryptal Myofibroblasts are Responsible for Increased TLR4 Expression in a Subset of CRCs. Double-stained immunofluorescence for TLR4 (green) and vimentin (red) in normal (I), adenoma (II), and colon adenocarcinoma (III) (10x). In the stromal compartment of CRCs, immunofluorescent staining for TLR4 localized to the pericryptal myofibroblasts in a subset of samples. Image collected and cropped by CiteAb from the following publication (http://www.jeccr.com/content/33/1/45), licensed under a CC-BY licence.](http://images.novusbio.com/images2/TLR4-Antibody-(76B357.1)-Immunohistochemistry-NB100-56566-img0040.jpg)
![Flow Cytometry: MIPEP Antibody (OTI5E7) [NBP2-45861] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by MIPEP antibody.](http://images.novusbio.com/images2/MIPEP-Antibody-(OTI5E7)-Flow-Cytometry-NBP2-45861-img0001.jpg)
![Immunofluorescence: MIPEP Antibody (OTI5E7) [NBP2-45861] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MIPEP.](http://images.novusbio.com/images2/MIPEP-Antibody-(OTI5E7)-Immunofluorescence-NBP2-45861-img0002.jpg)
![Immunohistochemistry: MIPEP Antibody (OTI5E7) [NBP2-45861] - Analysis of Human prostate tissue.](http://images.novusbio.com/images2/MIPEP-Antibody-(OTI5E7)-Immunohistochemistry-NBP2-45861-img0003.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-0 Antibody [NBP2-92773] - Human stomach using MIP antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/images2/Aquaporin-0-Antibody-Immunohistochemistry-Paraffin-NBP2-92773-img0001.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-0 Antibody [NBP2-92773] - Mouse brain using MIP antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/images2/Aquaporin-0-Antibody-Immunohistochemistry-Paraffin-NBP2-92773-img0002.jpg)
![Western Blot: Aquaporin-0 Antibody [NBP2-92773] - Analysis of extracts of various cell lines, using Aquaporin-0 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/images2/Aquaporin-0-Antibody-Western-Blot-NBP2-92773-img0004.jpg)
| Publication using NBP3-03233 | Applications | Species |
|---|---|---|
| Jia P, Xu S, Ren T et al. LncRNA IRAR regulates chemokines production in tubular epithelial cells thus promoting kidney ischemia-reperfusion injury Cell death & disease Jun 22 2022 [PMID: 35732633] (IHC-Fr, Mouse) | IHC-Fr | Mouse |
Secondary Antibodies |
Isotype Controls |
Diseases for CXCL2/GRO beta/MIP-2/CINC-3 Antibody (NBP3-03233)Discover more about diseases related to CXCL2/GRO beta/MIP-2/CINC-3 Antibody (NBP3-03233).
| Pathways for CXCL2/GRO beta/MIP-2/CINC-3 Antibody (NBP3-03233)View related products by pathway.
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| Gene Symbol | CXCL2 |
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on Jurkat cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.](http://images.novusbio.com/images2/NFkB-p105-p50-Antibody-(2J10D7)-Flow-Cytometry-NB100-56583-img0005.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on HeLa cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.](http://images.novusbio.com/images2/NFkB-p105-p50-Antibody-(2J10D7)-Flow-Cytometry-NB100-56583-img0006.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U2-OS cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/images2/NFkB-p105-p50-Antibody-(2J10D7)-Flow-Cytometry-NB100-56583-img0007.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")