CXCL2/GRO beta/MIP-2/CINC-3 Products

452-M2) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL2/MIP-2 Monoclonal Antibody (Catalog # MAB452). The ND₅₀ is typically 1-4 µg/mL." class="big_lightbox" target="_blank">

452-M2) was serially diluted and captured by Rat Anti-Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Monoclonal Antibody (Catalog # MAB452) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

452-M2) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL2/MIP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA). The ND₅₀ is typically 0.015‑0.075 µg/mL." class="big_lightbox" target="_blank">

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox" target="_blank">

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">

452-M2) was serially diluted and captured by Rat Anti-Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Monoclonal Antibody (Catalog # MAB452) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

525-C3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL2/ CINC-3 (0.1 µg/mL) is neutral-ized (green line) by increasing concentrations of Mouse Anti-Rat CXCL2/CINC-3 Monoclonal Antibody (Catalog # MAB525). The ND₅₀ is typically 0.4-2.0 µg/mL." class="big_lightbox" target="_blank">

525-C3) was serially diluted and captured by Mouse Anti-Rat CXCL2/GRO beta /MIP‑2/CINC‑3 Monoclonal Antibody (Catalog # MAB525) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat CXCL2/GRO beta /MIP‑2/CINC‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF525) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">