CXCL13/BLC/BCA-1 Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CXCL13/BLC/BCA-1 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse CXCL13/BLC (R&D Systems, Catalog # 470-BC)
  Ile22-Ala109
  Accession # Q3U1E8
• Specificity: Detects mouse CXCL13 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human CXCL13, recombinant mouse (rm) I-TAC, rmMIG, and rmSDF-1 alpha is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CXCL13
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse CXCL13/BLC/BCA-1 Antibody (Catalog # MAB470)
  ELISA Detection: Mouse CXCL13/BLC/BCA-1 Biotinylated Antibody (Catalog # BAF470)
  Standard: Recombinant Mouse CXCL13/BLC/BCA-1 (Catalog # 470-BC)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL13/BLC/BCA-1 Antibody [Biotin]

• ANGIE
• ANGIE2
• B cell-attracting chemokine 1
• B lymphocyte chemoattractant
• BCA1
• BCA-1
• BCA1B-cell chemoattractant
• BCA-1CXC chemokine BLC
• B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1)
• BLC
• BLCSmall-inducible cytokine B13
• BLR1L
• B-lymphocyte chemoattractant
• chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant)
• chemokine (C-X-C motif) ligand 13
• C-X-C motif chemokine 13
• CXCL13
• SCYB13
• SCYB13B-cell-attracting chemokine 1
• small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)

Background

CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. Mouse BCA-1 cDNA encodes a precursor protein of 109 amino acid residues with a putative leader sequence of 21 residues. Mature mouse BCA-1 shares 64% amino acid sequence similarity with the human protein and 23 - 34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

1. Gunn, M.D. et al. (1998) Nature, 391:799.
2. Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
3. Forster, R. et al. (1996) Cell 87:1037.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL13/BLC/BCA-1 Antibody (BAF470)(8)

Publications using BAF470

• HW Cheng, U Mörbe, M Lütge, C Engetschwi, L Onder, M Novkovic, C Gil-Cruz, C Perez-Shib, T Hehlgans, E Scandella, B Ludewig Intestinal fibroblastic reticular cell niches control innate lymphoid cell homeostasis and function Nature Communications, 2022-04-19;13(1):2027. 2022-04-19 [PMID: 35440118] (IHC, Mouse)
• Emma S. Crichton, Shan Zeng, G. Michael La Muraglia, I. Raul Badell CXCL13 Is an Indicator of Germinal Center Activity and Alloantibody Formation Following Transplantation Transplantation Direct 2021-11-05 [PMID: 34778545]
• Dana Bogdanova, Arata Takeuchi, Madoka Ozawa, Yasuhiro Kanda, M. Azizur Rahman, Burkhard Ludewig et al. Essential Role of Canonical NF-kappa B Activity in the Development of Stromal Cell Subsets in Secondary Lymphoid Organs The Journal of Immunology 2018-12-15 [PMID: 30397032]
• Arata Takeuchi, Madoka Ozawa, Yasuhiro Kanda, Mina Kozai, Izumi Ohigashi, Yoichi Kurosawa et al. A Distinct Subset of Fibroblastic Stromal Cells Constitutes the Cortex-Medulla Boundary Subcompartment of the Lymph Node Frontiers in Immunology 2018-10-02 [PMID: 30333825]
• Nakagawa R, Togawa A, Nagasawa T, Nishikawa S Peyer's patch inducer cells play a leading role in the formation of B and T cell zone architecture. J Immunol, 2013-03-04;190(7):3309-18. 2013-03-04 [PMID: 23460741] (IHC, Mouse)
• Baptista A, Olivier B, Goverse G, Greuter M, Knippenberg M, Kusser K, Domingues R, Veiga-Fernandes H, Luster A, Lugering A, Randall T, Cupedo T, Mebius R Colonic patch and colonic SILT development are independent and differentially regulated events. Mucosal Immunol, 2012-09-19;6(3):511-21. 2012-09-19 [PMID: 22990625] (IHC, Mouse)
• Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-01-25;58(2):169-77. 2012-01-25 [PMID: 22277799] (Luminex Development, Mouse)
• Katakai T, Suto H, Sugai M, Gonda H, Togawa A, Suematsu S, Ebisuno Y, Katagiri K, Kinashi T, Shimizu A Organizer-like reticular stromal cell layer common to adult secondary lymphoid organs. J. Immunol., 2008-11-01;181(9):6189-200. 2008-11-01 [PMID: 18941209] (IHC-Fr, Mouse)

Review for CXCL13/BLC/BCA-1 Antibody (BAF470) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-Fr Mouse 01/15/2015

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: See PMID: 22753937
• Species: Mouse

Bioinformatics

• Gene Symbol: CXCL13
• Uniprot:
  • Q3U1E8()