CX3CL1/Fractalkine Antibody - BSA Free

Western Blot: CX3CL1/Fractalkine Antibody [NBP1-76467] - C2C12 cell lysate with CX3CL1 antibody at (A) 0.5, (B) 1 and (C) 2 mg/ml.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CX3CL1/Fractalkine Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a peptide corresponding to 18 amino acids near the amino terminus of human CX3CL1. The immunogen is located within amino acids 20 - 70 of CX3CL1. Amino Acid Squence: CSKMTSKIPVALLIHYQQN
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CX3CL1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunoprecipitation
  • Western Blot 0.5-2 ug/ml
• Application Notes: CX3CL1 is ofen observed migrating at 80 - 100 kDa in SDS-PAGE, presumably due to post-translational modification.
• Control Peptide:

  CX3CL1/Fractalkine Peptide (NBP1-76467PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for CX3CL1/Fractalkine Antibody - BSA Free

• ABCD-3
• C3Xkine
• chemokine (C-X3-C motif) ligand 1
• CX3C membrane-anchored chemokine
• CX3CL1
• CXC3
• CXC3C
• FKN
• Fractalkine
• Neurotactin
• neurotactin)
• NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine
• NTTSmall-inducible cytokine D1
• SCYD1C-X3-C motif chemokine 1
• small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Background

Chemokines are a family of proteins associated with the trafficking of leukocytes in immune surveillance and inflammatory cell recruitment. They are classified based on the positions of key cysteine residues. CX3CL1 is a CX3C chemokine known to induce adhesion and migration of leukocytes mediated by a membrane-bound and soluble form respectively. Recent experiments have shown that CX3CL1 can suppress the production of nitrous oxide, interleukin-6, and TNF-alpha in activated microglia and neuronal cells, suggesting that it may act as an intrinsic inhibitor against neurotoxicity by activated microglia. Its receptor, CX3CR1, also functions as a co-receptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by CX3CL1. CX3CL1 is ofen observed migrating at 80 - 100kDa in SDS-PAGE, presumably due to post-translational modification.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CX3CL1
• Uniprot:
  • AAB50014(Human)