Human CTLA-4 ELISA Kit (Colorimetric)

Images

 
ELISA: CTLA-4 ELISA Kit [NBP1-91268] - CTLA-4 expression in human lung cancer cell lines determined by ELISA. Image from verified customer review.
ELISA: CTLA-4 ELISA Kit [NBP1-91268] - Representative standard curve for human sCTLA-4 ELISA. Human sCTLA-4 was diluted in serial 2-fold steps in Sample Diluent.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, heparin), amniotic fluid
Standard Curve Range
0.16 - 10.0 ng/mL
Sensitivity
0.13 ng/mL

Order Details

Human CTLA-4 ELISA Kit (Colorimetric) Summary

Specificity
The interference of circulating factors of the immune systemwas evaluated by spiking these proteins at physiologically relevant concentrations into a human CTLA-4 positive serum. There was no crossreactivity detected.
Standard Curve Range
0.16 - 10.0 ng/mL
Sensitivity
0.13 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
10.40%
Intra-Assay
4.80%
Spike Recovery
78%
Sample Volume
10 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CTLA4

Applications/Dilutions

Application Notes
The human sCTLA-4 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCTLA-4
Reviewed Applications
Read 1 Review rated 5
using
NBP1-91268 in the following application:

Publications
Read Publications using
NBP1-91268 in the following applications:

  • 2 publications
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCTLA-4
  3. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  4. Biotin-Conjugate anti-human sCTLA-4 monoclonal antibody
  5. Sample Diluent
  6. Human sCTLA-4 Standard lyophilized, 20 ng/mL upon reconstitution
  7. Stop Solution (1M Phosphoric acid)
  8. Streptavidin-HRP
  9. Substrate Solution (tetramethyl-benzidine)
  10. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Alternate Names for Human CTLA-4 ELISA Kit (Colorimetric)

  • CD
  • CD152 antigen
  • CD152
  • CD152IDDM12
  • CD28
  • celiac disease 3
  • CELIAC3
  • CTLA4
  • CTLA-4
  • cytotoxic T-lymphocyte antigen 4
  • cytotoxic T-lymphocyte protein 4
  • Cytotoxic T-lymphocyte-associated antigen 4
  • cytotoxic T-lymphocyte-associated protein 4
  • cytotoxic T-lymphocyte-associated serine esterase-4
  • GRD4
  • GSE
  • ICOS
  • ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4

Background

Cytotoxic T lymphocyte associated gene -4 (CTLA-4) was initially described as a classical type I glycoprotein on the surface of activated T-cells. CTLA-4 is a member of the Ig gene superfamily and along with its homologue, CD28, is a B7 binding protein. There is evidence that CTLA-4 is a negative regulator of T-cell activation, ligation of CTLA-4 on the T-cell surface initiates a series of biochemical events that attenuate an ongoing immune response. CTLA-4 knock out mice demonstrate profound polyclonal lymphoproliferative disoders that infiltrate most major organ systems and the animals die a few weeks after birth. The animals have increased levels of IgG which illustrates the role of CTLA-4 on humoral immune responses as well. A role for CTLA-4 in autoimmune disease is suggested by the observations that blockade of B7/CTLA-4 interaction exacerbates animal models of autoimmune disease such as experimental autoimmune encephalomyelitis and diabetes.A search for genetic markers that segregate with Graves? disease revealed an association with CTLA-4 polymorphisms. CTLA-4 is further more closely linked to autoimmune thyroid disease (ATD). A native soluble form of CTLA-4 has been described to be present in human serum. A correlation of circulating CTLA-4 and ATD has been shown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for CTLA-4 ELISA Kit (NBP1-91268)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ELISA, WB.


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Review for CTLA-4 ELISA Kit (NBP1-91268) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-91268:
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ELISA
(1)
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Images Ratings Applications Species Date Details
ELISA CTLA-4 NBP1-91268
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reviewed by:
Christopher England
ELISA Human 03/10/2016
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Summary

ApplicationELISA
Sample TestedHuman lung cancer cell lines
SpeciesHuman
Lot122662027

Blocking

Blocking DetailsThe exact protocol provided by Novus for this antibody kit was followed. No blocking details.

Primary Anitbody

Dilution RatioWe did not have to dilute our samples for ELISA. We only normalized the total protein to 20 ug.

Details

Detection NotesThe plate was read at 450 nm using the Biotek Synergy 4 plate reader.
ELISA Plate DetailsThe kit contained a plate pre-coated with sCTLA-4 antibody
Wash Description400 uL of the provided wash buffer was used to wash wells. We would let it sit for 10 s before aspiration. Washed 4-5X.

Comments

CommentsMake sure to read the protocol at least once all the way through before starting the ELISA. It only takes about 4-5 hours total time. Also, our standard curve did not correlate well with the standard curve provided in the protocol book (Yes, a 40 page book that is very detailed), so don't be surprised if you standard curve is off too. Our standard curve provided an r squared value > 0.98, so everything turned out great. Really liked the kit and will use it again in the future.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CTLA-4 ELISA Kit (NBP1-91268). (Showing 1 - 1 of 1 FAQs).

  1. Does this kit work with CTLA4Ig fusion protein? CTLA4Ig contains the extracellular domain of human CTLA4 and human IgG1 Fc. Do you know if any customers use this kit to check CTLA4Ig? sCTLA4 and CTLA4Ig have the same extracellular domain. If the two antibodies used in the ELISA kit are against the extracellular domain, it could be used to detect CTLA4Ig. Could you please tell me the epitopes of the two antibodies?
    • The standard protein in our human CTLA-4 is a soluble fusion protein consisting of the extracellular domain of human CTLA-4, fused to mouse IgG2a Fc. This means that our kit will detect your CTLA4Ig.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Christopher England
03/10/2016
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol CTLA4