CTGF/CCN2 Antibody (CL5339) - BSA Free

Western Blot: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Analysis in human cell line U-251 MG.

Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Staining of rat cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.

Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Staining of human cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.

Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.

Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Staining of mouse cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.

Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Staining of mouse cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.

Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Staining of rat cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: CL5339
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

CTGF/CCN2 Antibody (CL5339) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from P29279, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCN2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  CTGF/CCN2 Recombinant Protein Antigen (NBP2-61415PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for CTGF/CCN2 Antibody (CL5339) - BSA Free

• CCN2
• CCN2IGFBP-8
• connective tissue growth factor
• CTGF
• CTGRP
• Fisp12
• HCS24
• Hypertrophic chondrocyte-specific protein 24
• IBP-8
• IGF-binding protein 8
• IGFBP-8
• IGFBP8CCN family member 2
• Insulin-like growth factor-binding protein 8
• MGC102839
• NOV2

Background

Connective Tissue Growth Factor (CTGF) is a 38kDa, cysteine-rich, secreted peptide. CTGF promotes endothelial cell growth, migration, adhesion and survival. and is thus implicated in endothelial cell function and angiogenesis. CTGF is implicated in are embryogenesis, wound healing and regulation of extracellular matrix production, and is required for normal skeletal growth. CTGF antibodies are useful tools for angiogenesis and cell structure research, and for studies on certian types of cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CTGF/CCN2 Antibody (NBP2-61415). (Showing 1 - 1 of 1 FAQ).

1. We are looking for a pair of mouse CTGF antibody for ELISA, we prefer no azide and/or glycerol, and in carrier free form (no EDTA, no Tris, no BSA). For the validation, we need approximately 100ug. If it works, we'll order more later.
   • Unfortunately we do not carry any CTGF antibodies that suit your specifications. We do carry AbSelect antibody purification kits that will remove BSA, Tris and azide from antibody formulations which may be an option for you.

Bioinformatics

• Gene Symbol: CCN2