CTGF/CCN2 Antibody (88430) - C-terminus

CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Human CTGF/CCN2 C-Terminus Monoclonal Antibody (Catalog # MAB660) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 88430
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CTGF/CCN2 Antibody (88430) - C-terminus Summary

• Additional Information: C-terminal Peptide
• Immunogen: E. coli-derived recombinant human CTGF
  Glu247-Ala349
  Accession # NP_001892.1
• Specificity: Detects human CTGF C-teriminal peptide in direct ELISAs. Does not cross-react with recombinant human (rh) LDGF, rhNOV, rhPDGF, rhPlGF, rhVEGF₂₀₆, rhVEGF-B₁₈₆, rhVEGF-C, rhVEGF-D, or rhWISP-1.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CTGF
• Purity: Protein A or G purified from ascites
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Purity: Protein A or G purified from ascites
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CTGF/CCN2 Antibody (88430) - C-terminus

• CCN2
• CCN2IGFBP-8
• connective tissue growth factor
• CTGF
• CTGRP
• Fisp12
• HCS24
• Hypertrophic chondrocyte-specific protein 24
• IBP-8
• IGF-binding protein 8
• IGFBP-8
• IGFBP8CCN family member 2
• Insulin-like growth factor-binding protein 8
• MGC102839
• NOV2

Background

CTGF belongs to the CCN (CYR61/CTGF/NOV) family of secreted proteins that share a common multimodular organization. Each protein contains IGF-binding protein domain, a von Willebrand factor type C domain, a thrombospondin type I domain, and a cysteine knot domain. The multimodular CTGF has the ability to bind multiple ligands and has numerous effects on development, differentation, and disease. The C-terminal cysteine knot motif contains the heparin and low density lipoprotein receptor (LDLR) binding sites that contributes to the adhesive and mitogenic properties of CTGF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CTGF/CCN2 Antibody (MAB660)(6)

Publications using MAB660

• JT Chen, CY Wang, MH Chen Curcumin inhibits TGF-?1-induced connective tissue growth factor expression through the interruption of Smad2 signaling in human gingival fibroblasts J. Formos. Med. Assoc., 2018;0(0):. 2018 [PMID: 29339038] (IHC, Human)
• Yun Wang Human gingiva transcriptome during wound healing J. Clin. Periodontol, 2016;0(0):. 2016 [PMID: 28005267] (IHC, Human)
• Gao W, Cai L, Xu X, Fan J, Xue X, Yan X, Qu Q, Wang X, Zhang C, Wu G Anti-CTGF single-chain variable fragment dimers inhibit human airway smooth muscle (ASM) cell proliferation by down-regulating p-Akt and p-mTOR levels. PLoS ONE, 2014;9(12):e113980. 2014 [PMID: 25478966] (ELISA Development, Human)
• Alfaro , Maria P, Deskins , Desirae, Wallus , Meredith, DasGupta , Jayasri, Davidson , Jeffrey, Nanney , Lillian, A Guney , Michelle, Gannon , Maureen, Young , Pampee P A physiological role for connective tissue growth factor in early wound healing. Lab Invest, 2013;93(1):81-95. 2013 [PMID: 23212098] (Bioassay, Mouse)
• Chen , Pai-Shen, Wang , Ming-Yan, Wu , Shin-Ni, Su , Jen-Lian, Hong , Chih-Che, Chuang , Shuang-E, Chen , Min-Wei, Hua , Kuo-Tai, Wu , Yu-Ling, Cha , Shih-Tin, Babu , Munisamy, Chen , Chiung-N, Lee , Po-Huang, Chang , King-Jen, Kuo , Min-Lian CTGF enhances the motility of breast cancer cells via an integrin-alphavbeta3-ERK1/2-dependent S100A4-upregulated pathway. J Cell Sci, 2007;120(0):2053-65. 2007 [PMID: 17550972] (Western Blot, Human)
• Gronborg M, Kristiansen TZ, Iwahori A, Chang R, Reddy R, Sato N, Molina H, Jensen ON, Hruban RH, Goggins MG, Maitra A, Pandey A Biomarker discovery from pancreatic cancer secretome using a differential proteomic approach. Mol. Cell Proteomics, 2006;5(1):157-71. 2006 [PMID: 16215274] (Western Blot, Human)

FAQs for CTGF/CCN2 Antibody (MAB660). (Showing 1 - 1 of 1 FAQs).

1. We are looking for a pair of mouse CTGF antibody for ELISA, we prefer no azide and/or glycerol, and in carrier free form (no EDTA, no Tris, no BSA). For the validation, we need approximately 100ug. If it works, we'll order more later.
   • Unfortunately we do not carry any CTGF antibodies that suit your specifications. We do carry AbSelect antibody purification kits that will remove BSA, Tris and azide from antibody formulations which may be an option for you.

Bioinformatics

• Gene Symbol: CTGF
• Uniprot:
  • NP_001892.1()