CSDE1 Antibody

Western Blot: CSDE1 Antibody [NBP1-71915] - Purification of Csde1 containing RNP complexes. MEL cells expressing BirA, or expressing BirA plus biotag-Csde1 were lysed, and incubated with streptavidin beads. Supernatant and beads were collected, beads were washed and eluted. Western blot with fractions was stained with anti-Csde1 antibody. Size markers are indicated in kDa. The positions of endogenous and biotagged Csde1 are indicated. A single empty lane has been cropped. The uncropped image is available as Supplementary Figure S5. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-20518-7), licensed under a CC-BY license.

Western Blot: CSDE1 Antibody [NBP1-71915] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-UNR antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. UNR was also immunoprecipitated by rabbit anti-UNR antibodies NBP1-71913 and NBP1-71914, which recognize upstream epitopes.

Western Blot: CSDE1 Antibody [NBP1-71915] - Deletion of the 1st cold shock domain of Csde1 using Crispr/Cas9. Western blot stained for Csde1 and tubulin as loading control. The membrane was cut between Csde1 and tubulin and staining performed on each separately. D1 (2.1) and D2 (2.14) represent out-of-frame deletions (Del), H1 (2.121) and H2 (2.123) in-frame deletions of the 1st cold shock domain (Hm). HET are control clones that harbor 1 deleted (out-of-frame) allele and 1 wt allele. Raw image scans are available as Supplementary Figure S6. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-20518-7), licensed under a CC-BY license.

• Reactivity: Hu, Mu, Rt, Gp
• Applications: WB, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CSDE1 Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 748 and 798 of human Upstream of NRAS using the numbering given in entry NP_001007554.1 (GeneID 7812).
• Predicted Species: Rat (100%), Guinea Pig (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CSDE1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2- 10 ug/mg lysate
  • Knockdown Validated
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CSDE1 Antibody

• cold shock domain containing E1, RNA-binding
• cold shock domain-containing protein E1
• D1S155EUNRRP5-1000E10.3
• DKFZp779B0247
• DKFZp779J1455
• FLJ26882
• KIAA0885
• N-ras upstream gene protein
• NRAS-related
• NRU
• Protein UNR
• upstream of NRAS

Background

CSDE1, also known as Cold shock domain-containing protein E1, is a 798 amino acid long isoform that is 89 kDa and a short 767 amino acid isoform that is approx. 86 kDa, which is directly related to the translation of the human rhinovirus RNA as an RNA binding protein. Studies are being performed on the relationship of this protein to chronic lymphocytic leukemia, diamond-blackfan anemia, lymphocytic leukemia, breast cancer, and shock. CSDE1 protein involvement has been observed with relation to PCSK7, SYNCRIP, PABPC1, HNRNPD, PIK3R1 in the regulation of transcription DNA-dependent and male gonad development pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CSDE1 Antibody (NBP1-71915)(2)

Publications using NBP1-71915

• Moore KS, Yagci N, van Alphen F et al. Strap associates with Csde1 and affects expression of select Csde1-bound transcripts PLoS ONE 2018-08-23 [PMID: 30138317] (WB, Mouse)
• Moore KS, Yagci N, van Alphen F, Paolini NA. Csde1 binds transcripts involved in protein homeostasis and controls their expression in an erythroid cell line. Sci Rep. 2018-02-08 [PMID: 29422612] (WB, Mouse)

Bioinformatics

• Gene Symbol: CSDE1
• Uniprot:
  • NP_001007554.1(Human)