CSDE1 Antibody

CSDE1-Antibody-Western-Blot-NBP1-71915-img0004.jpg

Western Blot: CSDE1 Antibody [NBP1-71915] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-UNR antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. UNR was also immunoprecipitated by rabbit anti-UNR antibodies NBP1-71913 and NBP1-71914, which recognize upstream epitopes.

CSDE1-Antibody-Knockdown-Validated-NBP1-71915-img0003.jpg

• Reactivity: Hu, Mu, Rt, Gp
• Applications: WB, IP, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CSDE1 Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 748 and 798 of human Upstream of NRAS using the numbering given in entry NP_001007554.1 (GeneID 7812).
• Predicted Species: Rat (100%), Guinea Pig (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CSDE1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2- 10 ug/mg lysate
  • Knockdown Validated
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CSDE1 Antibody

• cold shock domain containing E1, RNA-binding
• cold shock domain-containing protein E1
• D1S155EUNRRP5-1000E10.3
• DKFZp779B0247
• DKFZp779J1455
• FLJ26882
• KIAA0885
• N-ras upstream gene protein
• NRAS-related
• NRU
• Protein UNR
• upstream of NRAS

Background

CSDE1, also known as Cold shock domain-containing protein E1, is a 798 amino acid long isoform that is 89 kDa and a short 767 amino acid isoform that is approx. 86 kDa, which is directly related to the translation of the human rhinovirus RNA as an RNA binding protein. Studies are being performed on the relationship of this protein to chronic lymphocytic leukemia, diamond-blackfan anemia, lymphocytic leukemia, breast cancer, and shock. CSDE1 protein involvement has been observed with relation to PCSK7, SYNCRIP, PABPC1, HNRNPD, PIK3R1 in the regulation of transcription DNA-dependent and male gonad development pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CSDE1 Antibody (NBP1-71915)(2)

Publications using NBP1-71915

• Moore KS, Yagci N, van Alphen F et al. Strap associates with Csde1 and affects expression of select Csde1-bound transcripts PLoS ONE 2018-08-23 [PMID: 30138317] (WB, Mouse)
• Moore KS, Yagci N, van Alphen F, Paolini NA. Csde1 binds transcripts involved in protein homeostasis and controls their expression in an erythroid cell line. Sci Rep. 2018-02-08 [PMID: 29422612] (WB, Mouse)

Bioinformatics

• Gene Symbol: CSDE1
• Uniprot:
  • NP_001007554.1(Human)