CRISP-2 Antibody - BSA Free

Western Blot: CRISP-2 Antibody [NBP1-76277] - Human testis tissue lysate with CRISP2 antibody at (A) 0.5 and (B) 1 ug/mL.

Immunohistochemistry: CRISP-2 Antibody - BSA Free [NBP1-76277] - Immunohistochemistry of CRISP-2 in human testis tissue with CRISP-2 antibody at 10 u/mL.

Immunocytochemistry/ Immunofluorescence: CRISP-2 Antibody - BSA Free [NBP1-76277] - Immunofluorescence of CRISP in human testis tissue with CRISP antibody at 20 u/mL.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CRISP-2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CRISP-2 Antibody - BSA Free (NBP1-76277) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human CRISP2. The immunogen is located within the first 50 amino acids of CRISP2. Amino Acid Squence: CPSLPAEGKDPAFTAL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRISP2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 0.5-1 ug/ml
• Control Peptide:

  CRISP-2 Peptide (NBP1-76277PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for CRISP-2 Antibody - BSA Free

• Cancer/testis antigen 36
• CRISP2
• CRISP-2
• CRISP-2GAPDL5
• CT36TSP1
• cysteine-rich secretory protein 2
• GAPDL5
• glyceraldehyde-3-phosphate dehydrogenase-like 5
• testis specific protein 1 (probe H4-1 p3-1)
• Testis-specific protein TPX-1
• TPX1
• TPX1MGC111136
• TSP1

Background

The cysteine-rich secretory proteins (CRISP) family is a group of four proteins that are strongly expressed in the male reproductive tract and have been implicated in having roles in male fertility. CRISP2, also known as TPX1, has been implicated in the adhesion between spermatids and Sertoli cells, and with CRISP1, is thought to be involved in sperm-egg fusion. CRISP2 has been shown to regulate the Ca2+ influx through ryanodine receptors (RYR) and may influence the acrosome reaction or sperm motility. CRISP2 has also been shown to bind to the mitogen-activated protein kinase kinase kinase 11 (MAP3K11) and localizes to the developing acrosome, suggesting this CRISP2-MAP3K11 complex may have a role in acrosome development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CRISP2
• Uniprot:
  • NP_001135880(Human)