CPOX Antibody - BSA Free

Western Blot: CPOX Antibody [NBP2-92779] - Analysis of extracts of various cell lines, using CPOX at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CPOX Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CPOX Antibody - BSA Free (NBP2-92779) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 111-454 of human CPOX (NP_000088.3). TSLGRPEEEEDELAHRCSSFMAPPVTDLGELRRRPGDMKTKMELLILETQAQVCQALAQVDGGANFSVDRWERKEGGGGISCVLQDGCVFEKAGVSISVVHGNLSEEAAKQMRSRGKVLKTKDGKLPFCAMGVSSVIHPKNPHAPTIHFNYRYFEVEEADGNKQWWFGGGCDLTPTYLNQEDAVHFHRTLKEACDQHGPDLYPKFKKWCDDYFFIAHRGERRGIGGIFFDDLDSPSKEEVFRFVQSCARAVVPSYIPLVKKHCDDSFTPQEKLWQQLRRGRYVEFNLLYDRGTKFGLFTPGSRIESILMSLPLTARWEYMHSPSENSKEAEILEVLRHPRDWVR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CPOX
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for CPOX Antibody - BSA Free

• Coprogen oxidase
• coproporphyrinogen oxidase
• coproporphyrinogenase
• coproporphyrinogen-III oxidase, mitochondrial
• COX
• CPO
• CPXcoproporphyrinogen oxidase (coproporphyria, harderoporphyria)
• EC 1.3.3.3
• HCP

Background

The protein encoded by the CPOX gene is the sixth enzyme of the heme biosynthetic pathway. The encoded enzyme is solubleand found in the intermembrane space of mitochondria. This enzyme catalyzes the stepwise oxidative decarboxylation ofcoproporphyrinogen III to protoporphyrinogen IX, a precursor of heme. Defects in this gene are a cause of hereditarycoproporphyria (HCP).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CPOX