COUP-TF II/NR2F2 Antibody - BSA Free

Western Blot: COUP-TF II/NR2F2 Antibody [NBP1-31980] - Sample (20 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:10000

• Reactivity: Mu, Hu, Rt, Bv, Ch, Sh, Xp, Ze
• Applications: WB, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

COUP-TF II/NR2F2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit COUP-TF II/NR2F2 Antibody - BSA Free (NBP1-31980) is a polyclonal antibody validated for use in WB. Anti-COUP-TF II/NR2F2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human COUP-TF II/NR2F2. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Human (100%), Rat (100%), Chicken (100%), Sheep (100%), Xenopus (100%), Bovine (100%), Zebrafish (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NR2F2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Knockdown Validated
  • Western Blot 1:5000-1:20000
• Application Notes: Knockdown validation (PMID: 31978970).
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (100%). Porcine reactivity reported in scientific literature (PMID:31978970).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for COUP-TF II/NR2F2 Antibody - BSA Free

• Apolipoprotein A-I regulatory protein 1
• ARP-1
• ARP1ADP-ribosylation factor related protein 1
• chicken ovalbumin upstream promoter transcription factor 2
• COUP transcription factor 2
• COUP transcription factor II
• COUPTF II
• COUP-TF II
• COUP-TF2
• COUPTFB
• COUPTFII
• COUP-TFII
• MGC117452
• NR2F2
• Nuclear receptor subfamily 2 group F member 2
• nuclear receptor subfamily 2, group F, member 2
• SVP40
• TFCOUP2apolipoprotein AI regulatory protein 1

Background

COUP-TFII, a NR2 Hepatocyte NF4-Like receptor, has been shown to affect neuronal development and differentiation, homeostasis in various human tissues, mesenchymal-epithelial interactions during organogenesis, and the development of the cardiovascular system. COUP-TFII interacts with and modulates several nuclear receptors, including estrogen receptor alpha (ER alpha), thyroid hormone receptors (TR), PPAR alpha, and retinoic acid receptors (RAR). Mice lacking COUP-TFII show abnormal heart and vascular development. COUP-TFII expression has been documented in human adrenal, lung, kidney, liver, spleen, skeletal muscle, and breast. ESTs have been isolated from human tissue libraries, including cancerous adrenal, bladder, brain, breast, colon, head/neck, kidney, lung, ovary, pancreas, pituitary, prostate, skeletal muscle, skin, smooth muscle, stomach, synovium, and uterus, and normal brain, adipose, adrenal, blood, brain, breast, cervix, ear, embryo, gallbladder, ganglion, heart, kidney, liver/spleen, lung, ovary, placenta, prostate, skin, spleen, testis, uterus, and vessel.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for COUP-TF II/NR2F2 Antibody (NBP1-31980)(1)

Publication using NBP1-31980

• Guo R, Chen F, Shi Z Suppression of Notch Signaling Stimulates Progesterone Synthesis by Enhancing the Expression of NR5A2 and NR2F2 in Porcine Granulosa Cells Genes (Basel) 2020-01-22 [PMID: 31978970] (KD, WB, Porcine)

Bioinformatics

• Gene Symbol: NR2F2