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EAR2/NR2F6 Antibody

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Western Blot: EAR2/NR2F6 Antibody [NBP2-24510] - Analysis of NR2F6 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-24510 at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Western Blot: EAR2/NR2F6 Antibody [NBP2-24510] - Analysis of NR2F6 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-24510 at 5 ug/ml. Goat anti-rabbit Ig HRP secondary ...read more

Product Details

Summary
Reactivity Hu, Ca, PmSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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EAR2/NR2F6 Antibody Summary

Immunogen
A portion of amino acids 20-70 of human NR2F6 was used as the immunogen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NR2F6
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5-1.0 ug/ml

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: 100% homologous in human, chimpanzee and monkey; 92% indog; 85% in cow and opossum; 78% in mouse, horse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EAR2/NR2F6 Antibody

  • EAR2
  • EAR-2ERBA-related gene-2
  • EAR2nuclear receptor subfamily 2 group F member 6
  • ERBAL2
  • ERBAL2v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 2
  • NR2F6
  • nuclear receptor subfamily 2, group F, member 6
  • V-erbA-related protein 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Ovalbumin Antibody [NB600-922] - Western blot detection of Ovalbumin from hen egg white using NB600-922 at 1:10,000. The blot was blocked ON at 4 degrees C in 3% BSA. The secondary antibody used was a DyLight 649 Conjugated Goat anti-rabbit IgG (H&L) diluted 1:20,000 and incubated 1 hr RT in blocking buffer for fluorescent western blotting.
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Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NB100-1596] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aromatase Antibody NB100-1596 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control rabbits. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control ovaries. Nonlabeling was observed when the primary antibody was omitted. Bars: 200 um (1st image) and 20 um (2nd image). Granulose cells, gc; theca cells, tc. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY licence.
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Western Blot: ARFRP1 Antibody [NBP2-14306] - Analysis in control (vector only transfected HEK293T lysate) and ARFRP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: ARFRP1 Antibody [NBP2-14306] - Staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells.
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Western Blot: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR2C2.
Immunohistochemistry: TR4/NR2C2 Antibody (4B1) [NBP2-46228] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
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Western Blot: Renin Antibody (2F10) [NBP2-45552] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Renin.
Immunohistochemistry: Renin Antibody (2F10) [NBP2-45552] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Immunohistochemistry: RNASE3 Antibody (5G4) [NBP2-76905] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Ribonuclease 3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
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Immunohistochemistry-Paraffin: Apolipoprotein A-II/ApoA2 Antibody (JB70-35) [NBP2-75410] - Analysis of paraffin-embedded rat big intestine tissue using anti-Apolipoprotein A II antibody. Counter stained with hematoxylin.
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Western Blot: XIAP Antibody - Unpurified [NB100-56183] - XIAP Antibody [NB100-56183] - Analysis of recombinant full-length IAP proteins using this antibody to XIAP at 1:2000. The data shows that the antibody recognizes only XIAP and not the other IAP proteins.
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Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - NR1D1 monoclonal antibody (M02), clone 4F6. Analysis of NR1D1 expression in NIH/3T3.
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Immunohistochemistry-Paraffin: SP1 Antibody [NB600-233] - Mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SP1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
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EAR2/NR2F6 Antibody
NBP2-24510
EAR2/NR2F6 Antibody
Species: Hu, Ca, Pm
Applications: WB
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Bioinformatics

Gene Symbol NR2F6