Complement Factor B Antibody - BSA Free

Western Blot: Complement Factor B Antibody [NBP2-15953] - Rat plasma (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Factor B antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Complement Factor B Antibody [NBP2-15953] - Human hepatoma, using Factor B antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: Complement Factor B Antibody [NBP2-15953] - Human plasma (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Factor B antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Complement Factor B Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Complement Factor B. The exact sequence is proprietary.
• Localization: Secreted
• Predicted Species: Chimpanzee (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CFB
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Complement Factor B Antibody - BSA Free

• BF AHUS4
• BF
• B-factor, properdin
• BFD
• C3 proaccelerator
• C3 proactivator
• C3/C5 convertase
• CFAB
• CFB
• Complement Factor B
• EC 3.4.21
• EC 3.4.21.47
• FB
• FBI12
• GBG
• GBGCFAB
• Glycine-rich beta glycoprotein
• glycine-rich beta-glycoprotein
• H2-Bf
• PBF2
• Properdin factor B

Background

This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

FAQs for Complement Factor B Antibody (NBP2-15953). (Showing 1 - 1 of 1 FAQ).

1. Why does your Complement Factor B Antibody [NBP2-15953] detect 2 bands by western Blot? The protein is 86 kDa and that band is clear. What is the second band running at ~60 kDa in your image? (Perhaps a degradation product or a different protein that cross reacts - do you know?
   • Thank you for contacting Novus regarding the NBP2-15953 CFB antibody. This protein has 2 isoforms that are both detected by this antibody. The canonical sequence has a molecular weight of 83 kD and the isoform 2 has a molecular weight of 68kD. See the uniprot summary: http://www.uniprot.org/uniprot/P00751

Bioinformatics

• Gene Symbol: CFB
• Uniprot:
  • P00751()