Complement C8A Antibody - BSA Free

Immunohistochemistry: Complement C8A Antibody - BSA Free [NBP3-47462] - Immunohistochemistry of paraffin-embedded Rat liver tissue slide using (Complement C8A antibody) at dilution of 1:200

• Reactivity: Hu, Rt
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Complement C8A Antibody - BSA Free Summary

• Immunogen: Complement component C8 alpha chain. (Uniprot# P07357)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: C8A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:50-1:500
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Complement C8A Antibody - BSA Free

• complement component 8, alpha polypeptide
• complement component C8 alpha chain

Background

C8 is synthesised in the liver, monocytes and fibroblasts and is a plasma protein consisting of three non identical polypeptide chains. The alpha-chain (64kDa) and gamma chain (22kDa) are disulphide linked, these associate non-covalently with the beta-chain (64kDa), to form the native C8 molecule with a molecular weight of 151kDa. Normal plasma concentration is 80mg/L.C8 is one of the terminal proteins of the complement pathways. C8 binds to the membrane bound C5b-7, C5b-8 combines with C9 to form the membrane attack complex (MAC). The MAC forms a hydrophilic, protein walled, transmembrane channel, formation of which leads to cell lysis and destruction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C8A