Complement Component C9 Antibody

Immunohistochemistry: Complement Component C9 Antibody [NBP2-15952] - A generic staining of the vascular BMs was given by an antibody to the 7S domain of collagen IV a3 (C). Prominent staining for microvascular aneurisms was detected by staining with antibodies to C9 (B, C). The same treatment of vascular BM whole mounts from non-diabetic eyes did not show staining for these proteins (D). Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0189857) licensed under a CC-BY license.

Western Blot: Complement Component C9 Antibody [NBP2-15952] - Sample (30 ug of whole cell lysate) A: THP-1 7. 5% SDS PAGE gel, diluted at 1:500.

Immunocytochemistry/Immunofluorescence: Complement Component C9 Antibody [NBP2-15952] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C9 stained by C9 antibody [N2C2-2], Internal diluted at 1:500. Blue: Hoechst 33342 staining.

Western Blot: Complement Component C9 Antibody [NBP2-15952] - Human plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C9 antibody [N2C2-2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Complement Component C9 Antibody [NBP2-15952] - Rat plasma (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Complement Component C9 Antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Complement Component C9 Antibody [NBP2-15952] - Mouse plasma (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Complement Component 9 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody,

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Complement Component C9 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human C9. The exact sequence is proprietary.
• Localization: Membrane; Multi-pass membrane protein; Secreted; Membrane; Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: C9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Western Blot 1:500-1:3000
• Theoretical MW: 63 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol (pH7).
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for Complement Component C9 Antibody

• complement component 9
• Complement Component C9

Background

This gene encodes the final component of the complement system. It participates in the formation of the Membrane Attack Complex (MAC). The MAC assembles on bacterial membranes to form a pore, permitting disruption of bacterial membrane organization. Mutations in this gene cause component C9 deficiency. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Complement Component C9 Antibody (NBP2-15952)(1)

Publication using NBP2-15952

• Halfter W, Moes S, Asgeirsson DO et al. Diabetes-related changes in the protein composition and the biomechanical properties of human retinal vascular basement membranes. PLoS ONE 2017-12-28 [PMID: 29284024] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: C9
• Uniprot:
  • P02748()