Complement C3d Antibody (053A-1149.3.1.4)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, IHC-Fr
• Clone: 053A-1149.3.1.4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Complement C3d Antibody (053A-1149.3.1.4) Summary

• Immunogen: Purified C3d from human plasma
• Specificity: NB100-65510 is specific for human complement component 3d (C3d), a 33kDa polypeptide fragment generated over the course of complement activation where complement 3 (C3) convertases cleave C3 to C3b, which is further degraded into iC3b and C3dg/C3d. C3d is involved in the regulation of many aspects of the immune response, including antigen processing and presentation. The C3d/antigen complex mediates B cell activation by simultaneously binding antigen-specific surface bound immunoglobulin and CD21, lowering the complement activation threshold. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: C3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: BBS (pH 8.4)
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Complement C3d Antibody (053A-1149.3.1.4)

• AHUS5
• ARMD9
• ASP
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
• complement C3
• complement component 3
• complement component C3
• CPAMD1acylation-stimulating protein cleavage product

Background

The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C3
• Entrez:
  • 718(Human)
• Uniprot:
  • P01024()