Complement C3c Antibody (10-02A) - BSA Free

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Immunohistochemistry-Frozen: Complement C3c Antibody (10-02A) [NB100-64339] - Human dermis stained with Complement C3c Antibody. IHC-Fr image submitted by a verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-Fr
Clone
10-02A
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Complement C3c Antibody (10-02A) - BSA Free Summary

Immunogen
Human C3 from normal serum, highly purified by chromatography.
Specificity
NB100-64339 recognizes 135 kDa complement component 3c (C3c), present in blood serum. C3c is generated over the course of complement activation, where convertase C4b2a (classical pathway) and convertase C3bBb (alternative pathway) cleave C3 to C3b and C3a. C3b is further degraded into iC3b and C3dg/C3d. iC3b is then slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
C3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
In Western Blot binds both C3c and C3 and so is not suitable for all ELISA applications.
Reviewed Applications
Read 1 Review rated 5
using
NB100-64339 in the following applications:

Publications
Read Publication using NB100-64339.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Borate buffered saline (pH 8.4)
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Complement C3c Antibody (10-02A) - BSA Free

  • AHUS5
  • ARMD9
  • ASP
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
  • complement C3
  • complement component 3
  • complement component C3
  • CPAMD1acylation-stimulating protein cleavage product

Background

The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Complement C3c Antibody (NB100-64339)(1)

Review for Complement C3c Antibody (NB100-64339) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-64339:
Filter by Applications
Immunohistochemistry-Frozen
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Complement C3c NB100-64339
Enlarge
5
reviewed by:
Layla Schwartz
Immunohistochemistry-Frozen Human 08/06/2021
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedDermis
SpeciesHuman
Lot10-02A

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Secondary Antibodies

 

Isotype Controls

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Array NB100-64339

Bioinformatics Tool for Complement C3c Antibody (NB100-64339)

Discover related pathways, diseases and genes to Complement C3c Antibody (NB100-64339). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Layla Schwartz
08/06/2021
Application: Immunohistochemistry-Frozen
Species: Human

Bioinformatics

Gene Symbol C3
Entrez
Uniprot