Collagen IV alpha 1 Antibody

Images

 
Immunohistochemistry-Paraffin: Collagen IV alpha 1 Antibody [NB120-6586] - Analysis using the Biotin conjugate of NB120-6586. Staining of Collagen IV alpha 1 (red) and Insulin (green) in mouse pancreas. Image from ...read more
Immunohistochemistry-Paraffin: Collagen IV alpha 1 Antibody [NB120-6586] - Analysis of Collagen IV in various paraffin embedded human tissues. Image courtesy of product review submitted by Daniel Pirici.
Immunohistochemistry-Paraffin: Collagen IV alpha 1 Antibody [NB120-6586] - Used at a dilution of 1:400, 45 min RT showed strong staining in FFPE sections of human kidney (Left) with strong red staining observed in ...read more
Immunohistochemistry-Paraffin: Collagen IV alpha 1 Antibody [NB120-6586] - Analysis of Collagen IV alpha 1 in paraffin embedded mouse cervix tissue using anti-Collagen IV alpha 1 antibody. Image from verified customer ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, MaSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Catalog# & Conjugate Size Price

Collagen IV alpha 1 Antibody Summary

Immunogen
Collagen Type IV from human and bovine placenta. Uniprot ID P02462
Epitope
The development of type-specific antibodies is dependent on NON-DENATURED three-dimensional epitopes.
Localization
Secreted and Cell surface (Potential).
Specificity
Negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COL4A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • ELISA 1:5000-1:50000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:100
Application Notes
Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and western blotting for highly sensitive qualitative analysis. Greatly diminished reactivity and selectivity of the antibody will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Use in frozen sections reported in scientific literature (PMID 28672034). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID : 29162797).
Positive Control
Collagen IV alpha 1 Lysate (NBP2-64939)
Reviewed Applications
Read 4 Reviews rated 4.8
using
NB120-6586 in the following applications:

Publications
Read Publications using
NB120-6586 in the following applications:

Reactivity Notes

This antibody is expected to react with Type IV collagens from most mammalian species. Rat reactivity reported in scientific literature (PMID: 16774905)

Packaging, Storage & Formulations

Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Buffer
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Collagen IV alpha 1 Antibody

  • alpha-1 chain
  • Arresten
  • COL4A1 NC1 domain
  • COL4A1
  • collagen alpha-1(IV) chain
  • Collagen IV alpha 1
  • collagen IV, alpha-1 polypeptide
  • collagen, type IV, alpha 1
  • Collagen-4
  • EC 3.4.23
  • EC 6.3.1.2

Background

This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB120-6586
Species: Hu, Mu, Rt, Bv, Ma
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for Collagen IV alpha 1 Antibody (NB120-6586)(10)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Humans.

We have publications tested in 3 applications: ICC/IF, IHC-FR, IHC-P.


Filter By Application
ICC/IF
(1)
IHC-FR
(1)
IHC-P
(3)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
Rat
(2)
Humans
(1)
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Showing Publications 1 - 10 of 10.
Publications using NB120-6586 Applications Species
Nikolaou S, Hadjikypri X, Ioannou G et al. Functional and phenotypic distinction of the first two trophoblast subdivisions and identification of the border between them during early postimplantation: A prerequisite for understanding early patterning during placentogenesis Biochem. Biochem. Biophys. Res. Commun. 2018 Jan 29 [PMID: 29305264] (IHC-P)

Details:
This citation used the Biotin form of this antbody
IHC-P
Mogoanta L, Ciurea M, Pirici I et al. Different dynamics of aquaporin 4 and glutamate transporter 1 distribution in the perineuronal and perivascular compartments during ischemic stroke. Brain Pathol. 2014 Feb 27 [PMID: 24571080] (IHC-P, Human) IHC-P Human
Pirici D, Pirici I, Mogoanta L et al. Matrix metalloproteinase-9 expression in the nuclear compartment of neurons and glial cells in aging and stroke Neuropathology 2012 Oct [PMID: 22151540] (IHC-P, Rat) IHC-P Rat
Xu, H, Zeng, L, Peng, H, Chen, S, Jones, J, Chew, T-L, Sadeghi, M M, Kanwar, Y S, Danesh, F R. HMG-CoA reductase inhibitor simvastatin mitigates VEGF-induced quot;inside-outquot; signaling to extracellular matrix by preventing RhoA activation,. 291, F995-1004. 2006 [PMID: 16774905]
Root-Bernstein R. Rethinking Molecular Mimicry in Rheumatic Heart Disease and Autoimmune Myocarditis: Laminin, Collagen IV, CAR, and B1AR as Initial Targets of Disease Pediatric Cardiology 2014 Aug 19
Velez DO, Tsui B, Goshia T et al. 3D collagen architecture induces a conserved migratory and transcriptional response linked to vasculogenic mimicry Nat Commun. 2017 Nov 21 [PMID: 29162797] (ICC/IF, Humans) ICC/IF Humans
Lin EY, Bayarsengee U, Wang CC et al. The natural compound 2,3,5,4'-tetrahydroxystilbene-2-O-b-d glucoside protects against adriamycin-induced nephropathy through activating the Nrf2-Keap1 antioxidant pathway. Environ. Toxicol. 2017 Oct 24 [PMID: 29064158] (Mouse) Mouse
Bracaglia LG, Messina M, Winston S et al. 3D Printed Pericardium Hydrogels To Promote Wound Healing in Vascular Applications. Biomacromolecules. 2017 Oct 16 [PMID: 28976740] (Rat) Rat
Liguori R, Incensi A, de Pasqua S et al. Skin globotriaosylceramide 3 deposits are specific to Fabry disease with classical mutations and associated with small fibre neuropathy PLoS ONE 2017 Jul 03 [PMID: 28672034] (IHC-FR, Human) IHC-FR Human
Root-Bernstein R Rethinking Molecular Mimicry in Rheumatic Heart Disease and Autoimmune Myocarditis: Laminin, Collagen IV, CAR, and B1AR as Initial Targets of Disease. Pediatric Cardiology 2014 Aug 19 [PMID: 25191648]

Reviews for Collagen IV alpha 1 Antibody (NB120-6586) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Mouse, Feline.

Reviews using NB120-6586:
Filter by Applications
IHC-Fr
(1)
IHC-P
(3)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
Feline
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Collagen IV alpha 1 NB120-6586
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reviewed by:
Anonymous
IHC-Fr Feline 02/18/2018
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedConnective tissue
SpeciesFeline
Lot37798
Immunohistochemistry-Paraffin Collagen IV alpha 1 NB120-6586
Enlarge
reviewed by:
Anonymous
IHC-P Mouse 04/27/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse cervix
SpeciesMouse
 
reviewed by:
Anonymous
IHC-P 09/15/2012
View

Summary

ApplicationImmunohistochemistry-Paraffin
Special ApplicationsAll the stained tissue was tested in human
Pub Med ID22151540
FileView PDF
Immunohistochemistry-Paraffin Collagen IV alpha 1 NB120-6586
Enlarge
reviewed by:
Anonymous
IHC-P Human 09/15/2012
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman tissue
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Collagen IV alpha 1 Antibody (NB120-6586) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Collagen IV alpha 1.

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Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

Anonymous
02/18/2018
Application: IHC-Fr
Species: Feline

Anonymous
04/27/2016
Application: IHC-P
Species: Mouse

 
Anonymous
09/15/2012
Application: IHC-P
Species:

Bioinformatics

Gene Symbol COL4A1
Entrez
Uniprot