Immunohistochemistry-Frozen: Collagen IV alpha 1 Antibody [NB120-6586] - Mouse liver tissue. Collagen IV alpha 1 antibody at 1:200 dilution. Overnight incubation, no permeabilization. IHC-Fr image submitted by a ...read more
Immunohistochemistry-Paraffin: Collagen IV alpha 1 Antibody [NB120-6586] - Analysis of Collagen IV in various paraffin embedded human tissues. Image courtesy of product review submitted by Daniel Pirici.
Immunohistochemistry-Paraffin: Collagen IV alpha 1 Antibody [NB120-6586] - Used at a dilution of 1:400, 45 min RT showed strong staining in FFPE sections of human kidney (Left) with strong red staining observed in ...read more
Immunohistochemistry-Paraffin: Collagen IV alpha 1 Antibody [NB120-6586] - Analysis of Collagen IV alpha 1 in paraffin embedded mouse cervix tissue using anti-Collagen IV alpha 1 antibody. IHC-P image submitted by a ...read more
Immunohistochemistry-Paraffin: Collagen IV alpha 1 Antibody [NB120-6586] - Analysis using the Biotin conjugate of NB120-6586. Staining of Collagen IV alpha 1 (red) and Insulin (green) in mouse pancreas. IHC-P image ...read more
Collagen Type IV from human and bovine placenta. Uniprot ID P02462
The development of type-specific antibodies is dependent on NON-DENATURED three-dimensional epitopes.
Secreted and Cell surface (Potential).
Negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and western blotting for highly sensitive qualitative analysis. Greatly diminished reactivity and selectivity of the antibody will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Use in frozen sections reported in scientific literature (PMID 28672034). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID : 29162797).
This antibody is expected to react with Type IV collagens from most mammalian species. Rat reactivity reported in scientific literature (PMID: 16774905)
Packaging, Storage & Formulations
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% Sodium Azide
Immunogen affinity purified
Alternate Names for Collagen IV alpha 1 Antibody
COL4A1 NC1 domain
collagen alpha-1(IV) chain
Collagen IV alpha 1
collagen IV, alpha-1 polypeptide
collagen, type IV, alpha 1
This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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