Collagen I/III Native Protein

Product Discontinued

Collagen I/III Native Protein Summary

• Description: Native mouse collagen I/III
  
  

  Source:Murine tail tendons

  Amino Acid Sequence:UniProt P08121, P11087, Q01149

• Preparation Method: Pepsin treatment, acetic acid extraction, serial salt precipitations.
• Protein/Peptide Type: Native Protein
• Gene: COL1A1

Applications/Dilutions

• Dilutions:
  • ELISA
• Theoretical MW: 300 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. After reconstitution store at -20C.
• Buffer: Essentially salt free
• Concentration: LYOPH
• Reconstitution Instructions: Use 0.05-0.5 M acetic acid, pH 2.5 at 4C. Dissolved collagen retains immunologic properties of native collagen types I and III. Thermal denaturation converts dissolved collagen to gelatin.

Alternate Names for Collagen I/III Native Protein

• Alpha-1 type I collagen
• Collagen 1/3
• collagen alpha 1 chain type I
• collagen alpha-1(I) chain
• collagen of skin, tendon and bone, alpha-1 chain
• collagen, type I, alpha 1
• OI4
• pro-alpha-1 collagen type 1

Background

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: COL1A1