Collagen I/III Antibody

• Reactivity: Bv
• Applications: ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Collagen I/III Antibody Summary

• Immunogen: Bovine collagen type I and III from skin
• Specificity: NB100-64523 reacts with both Collagen I and III. Less than 0.5% reactivity was observed with bovine albumin and immunoglobulins. Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COL1A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:40

Reactivity Notes

Cross reacts with Bovine.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 1% Dextran, 1% Mannitol
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Collagen I/III Antibody

• Alpha-1 type I collagen
• Collagen 1/3
• collagen alpha 1 chain type I
• collagen alpha-1(I) chain
• collagen of skin, tendon and bone, alpha-1 chain
• collagen, type I, alpha 1
• OI4
• pro-alpha-1 collagen type 1

Background

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen I/III Antibody (NB100-64523)(1)

Publication using NB100-64523

• Yu M, Agarwal D, Korutla L et al. Islet transplantation in the subcutaneous space achieves long-term euglycaemia in preclinical models of type 1 diabetes Nat Metab 2020-09-07 [PMID: 32895576]

FAQs for Collagen I/III Antibody (NB100-64523). (Showing 1 - 1 of 1 FAQ).

1. In which buffer would you recommend to reconstitute it?
   • For the Collagen I/III antibody (NB100-64523) I am showing that you should use 500 uL sterile distilled water or saline.

Bioinformatics

• Gene Symbol: COL1A1
• Entrez:
  • 282187(Bovine)
• Uniprot:
  • P02453(Bovine)