Collagen I/II/III/IV/V Antibody (ST58-04)

Western Blot: Collagen I/II/III/IV/V Antibody (ST58-04) [NBP2-67538] - Analysis of Collagen I/II/III/IV/V on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: Collagen I/II/III/IV/V Antibody (ST58-04) [NBP2-67538] - Analysis of paraffin-embedded human uterus tissue using anti-COL1A1 antibody. Counter stained with hematoxylin.

Western Blot: Collagen I/II/III/IV/V Antibody (ST58-04) [NBP2-67538] - Analysis of COL1A1 on human placenta lysates using anti-COL1A1 antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: Collagen I/II/III/IV/V Antibody (ST58-04) [NBP2-67538] - Analysis of paraffin-embedded human kidney tissue using anti-COL1A1 antibody. Counter stained with hematoxylin.

Product Discontinued

Collagen I/II/III/IV/V Antibody (ST58-04) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Collagen I/II/III/IV/V aa 1991-1238 / 1464. (SwissProt: P02452 Human; SwissProt: P08123 Human; Entrez Gene:282187 Cow; Entrez Gene:282188 Cow)
• Localization: Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: COL1A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Collagen I/II/III/IV/V Antibody (ST58-04)

• Alpha-1 type I collagen
• Collagen 1/2/3/4/5
• collagen alpha 1 chain type I
• collagen alpha-1(I) chain
• collagen of skin, tendon and bone, alpha-1 chain
• collagen, type I, alpha 1
• OI4
• pro-alpha-1 collagen type 1

Background

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COL1A1