Collagen I Antibody [FITC]

Images

 
Western Blot: Collagen I alpha 1 Antibody [FITC] [NBP2-46874] - Lane 1: Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced). Lane 2: PPARg transduced cell lysates. Load: 100 ug per lane
Immunohistochemistry: Collagen I alpha 1 Antibody [FITC] [NBP2-46874] - Tissue: Human Skin at pH9. Fixation: formalin fixed paraffin embedded. Primary antibody: Collagen Type I antibody at 10 ug/mL for 1 h at RT. ...read more
Immunohistochemistry: Collagen I alpha 1 Antibody [FITC] [NBP2-46874] - Tissue: Human Skin at pH6. Fixation: formalin fixed paraffin embedded. Primary antibody: Collagen Type I antibody at 10 ug/mL for 1 h at RT. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Fe, RbSpecies Glossary
Applications WB, ELISA, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
FITC
Concentration
LYOPH

Order Details

Collagen I Antibody [FITC] Summary

Immunogen
Collagen I from human and bovine placenta. Uniprot ID P02452
Localization
Extracellular matrix
Specificity
Typically less than 1% cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:3000 - 1:6000
  • ELISA 1:3000-1:6000
  • Immunohistochemistry 1:50 - 1:200
  • Immunoprecipitation 1:100
Theoretical MW
139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected to react with most mammalian Type I collagens. Porcine reactivity reported in scientific literature (PMID: 21688145). Rabbit reactivity reported in scientific literature (PMID: 27663536).

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 50ul deionized water.

Alternate Names for Collagen I Antibody [FITC]

  • Collagen I

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

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Uniprot