COASY Antibody - BSA Free

Western Blot: COASY Antibody [NBP2-15934] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: COASY Antibody [NBP2-15934] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: COASY Antibody [NBP2-15934] - Immunohistochemical analysis of paraffin-embedded Colon carcinoma, using antibody at 1:500 dilution.

• Reactivity: Hu, Po
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

COASY Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human COASY. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Porcine (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COASY
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 62 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (87%), Rat (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for COASY Antibody - BSA Free

• bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase
• CoA synthaseUKR1
• CoASY
• Coenzyme A synthase
• DPCK
• FLJ35179
• NBPbifunctional coenzyme A synthase
• nucleotide binding protein
• phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase
• pOV-2
• PPAT

Background

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for COASY Antibody (NBP2-15934)(1)

Publication using NBP2-15934

• Arif A, Jia J, Willard B et al. Multisite Phosphorylation of S6K1 Directs a Kinase Phospho-code that Determines Substrate Selection. Mol. Cell 2018-12-27 [PMID: 30612880] (WB, Human)

Bioinformatics

• Gene Symbol: COASY
• Uniprot:
  • Q13057(Human)