COASY Antibody - BSA Free

Western Blot: COASY Antibody [NBP1-32649] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE, antibody diluted at 1:3000.

Immunocytochemistry/Immunofluorescence: COASY Antibody [NBP1-32649] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: COASY Antibody [NBP1-32649] - COASY antibody detects COASY protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. COASY antibody diluted at 1:500.

Western Blot: COASY Antibody [NBP1-32649] - (30 ug whole cell lysate) A: HeLa S3 7.5% SDS PAGE diluted at 1:1000

Immunohistochemistry-Paraffin: COASY Antibody [NBP1-32649] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: COASY Antibody [NBP1-32649] - COASY Antibody detects COASY protein at cytoplasm in mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. COASY antibody diluted at 1:500.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

COASY Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit COASY Antibody - BSA Free (NBP1-32649) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human COASY. The exact sequence is proprietary.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COASY
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 62 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for COASY Antibody - BSA Free

• bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase
• CoA synthaseUKR1
• CoASY
• Coenzyme A synthase
• DPCK
• FLJ35179
• NBPbifunctional coenzyme A synthase
• nucleotide binding protein
• phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase
• pOV-2
• PPAT

Background

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: COASY