CNTF Antibody [Unconjugated]

CNTF was detected in perfusion fixed frozen sections of mouse cerebellum using Goat Anti-Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

CNTF was detected in perfusion fixed frozen sections of rat cerebellum using Goat Anti-Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

CNTF was detected in perfusion fixed paraffin-embedded sections of mouse brain (hippocampus) using Goat Anti-Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm of Purkinje cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Recom­binant Rat CNTF (Catalog # 557-NT) stimulates proliferation in the TF‑1 human erythro­leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat CNTF (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA). The ND50 is typ­ically 0.3-2.4 µg/mL.

• Reactivity: Mu, Rt
• Applications: IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CNTF Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant rat CNTF
  Ala2-Met200
  Accession # P20294.1
• Specificity: Detects rat CNTF in direct ELISAs and Western blots. Detects mouse and rat CNTF in immunohistochemistry. In direct ELISAs and Western blots, less than 15% cross-reactivity with recombinant human CNTF is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CNTF
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 4-8 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CNTF Antibody [Unconjugated]

• ciliary neurotrophic factor
• CNTF
• HCNTF

Background

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons, and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. The cDNA for CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CNTF Antibody (AF-557-NA)(12)

Publications using AF-557-NA

• Kakae, M;Nakajima, H;Tobori, S;Kawashita, A;Miyanohara, J;Morishima, M;Nagayasu, K;Nakagawa, T;Shigetomi, E;Koizumi, S;Mori, Y;Kaneko, S;Shirakawa, H; The astrocytic TRPA1 channel mediates an intrinsic protective response to vascular cognitive impairment via LIF production Science advances 2023-07-21 [PMID: 37478173] (Immunohistochemistry, Mouse, Transgenic Mouse)
• Meifan Chen, Laura Ingle, Erik J. Plautz, Xiangmei Kong, Rui Tang, Neil Ghosh et al. LZK-dependent stimulation of astrocyte reactivity promotes corticospinal axon sprouting Frontiers in Cellular Neuroscience 9/15/2022 [PMID: 36187291]
• P Gómez-Fern, A Lopez de L, I Astobiza, J Mena, A Urtasun, V Altmann, F Matesanz, D Otaegui, E Urcelay, A Antigüedad, S Malhotra, X Montalban, T Castillo-T, L Espino-Pai, O Aktas, M Buttmann, A Chan, B Fontaine, PA Gourraud, M Hecker, S Hoffjan, C Kubisch, T Kümpfel, F Luessi, UK Zettl, F Zipp, I Alloza, M Comabella, CM Lill, K Vandenbroe The Rare IL22RA2 Signal Peptide Coding Variant rs28385692 Decreases Secretion of IL-22BP Isoform-1, -2 and -3 and Is Associated with Risk for Multiple Sclerosis Cells, 2020-01-10;9(1):. 2020-01-10 [PMID: 31936765] (Western Blot, Mouse)
• GJ Moon, S Kim, MT Jeon, KJ Lee, IS Jang, M Nakamura, SR Kim Therapeutic Potential of AAV1-Rheb(S16H) Transduction Against Alzheimer&#039;s Disease J Clin Med, 2019-11-22;8(12):. 2019-11-22 [PMID: 31766645] (IHC, Mouse)
• Alán Alpár, Péter Zahola, János Hanics, Zsófia Hevesi, Solomiia Korchynska, Marco Benevento et al. Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute stress The EMBO Journal 11/2/2018 [PMID: 30209240]
• Martina Senzacqua, Ilenia Severi, Jessica Perugini, Samantha Acciarini, Saverio Cinti, Antonio Giordano Action of Administered Ciliary Neurotrophic Factor on the Mouse Dorsal Vagal Complex Frontiers in Neuroscience 6/27/2016 [PMID: 27445662]
• Duo Jin, Yuanyuan Liu, Fang Sun, Xuhua Wang, Xuefeng Liu, Zhigang He Restoration of skilled locomotion by sprouting corticospinal axons induced by co-deletion of PTEN and SOCS3 Nature Communications 11/24/2015 [PMID: 26598325]
• Severi I, Perugini J, Mondini E, Smorlesi A, Frontini A, Cinti S, Giordano A Opposite effects of a high-fat diet and calorie restriction on ciliary neurotrophic factor signaling in the mouse hypothalamus. Front Neurosci, 2013-12-27;7(0):263. 2013-12-27 [PMID: 24409114] (IHC, Mouse)
• Jason M. Askvig, David Y. Lo, Adam W. Sudbeck, Kathryn E. Behm, Laura J. Leiphon, John A. Watt Inhibition of the Jak-STAT pathway prevents CNTF-mediated survival of axotomized oxytocinergic magnocellular neurons in organotypic cultures of the rat supraoptic nucleus Experimental Neurology 2/1/2013 [PMID: 23123407]
• Jason M. Askvig, Laura J. Leiphon, John A. Watt Neuronal activity and axonal sprouting differentially regulate CNTF and CNTF receptor complex in the rat supraoptic nucleus Experimental Neurology 1/1/2012 [PMID: 22037350]
• Leaver SG, Cui Q, Plant GW, Arulpragasam A, Hisheh S, Verhaagen J, Harvey AR AAV-mediated expression of CNTF promotes long-term survival and regeneration of adult rat retinal ganglion cells. Gene Ther., 2006-05-18;13(18):1328-41. 2006-05-18 [PMID: 16708079] (IHC, Mouse)
• Gard AL, Gavin E, Solodushko V, Pennica D Cardiotrophin-1 in choroid plexus and the cerebrospinal fluid circulatory system. Neuroscience, 2004-01-01;127(1):43-52. 2004-01-01 [PMID: 15219667] (Western Blot, Mouse, Rat)

Bioinformatics

• Gene Symbol: CNTF
• Uniprot:
  • P20294.1()