Claspin Antibody (485508)

Western blot shows lysates of HCT-116 human colorectal carcinoma cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Human Claspin Monoclonal Antibody (Catalog # MAB3310) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Claspin at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 485508
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Claspin Antibody (485508) Summary

• Immunogen: E. coli-derived recombinant human Claspin
  Phe1138-Ser1339
  Accession # Q9HAW4
• Specificity: Detects endogenous human Claspin in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLSPN
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Claspin Antibody (485508)

• claspin homolog (Xenopus laevis)
• claspin homolog
• Claspin
• CLSPN
• hClaspin
• MGC131612
• MGC131613
• MGC131615

Background

Claspin is a nuclear protein whose expression peaks during S and G2 phases of the cell cycle. Claspin is phosphorylated by ATR in response to DNA damage or replication stress. It functions in cell cycle checkpoint control by regulating the activation of Chk1 and BRCA1. Claspin contains three CKB motifs and two coiled coil regions. Over the range used for immunization, human and mouse Claspin share 72% amino acid sequence identity. DNA replication and DNA damage induce the phosphorylation of Claspin by Chk1-dependent mechanisms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Claspin Antibody (MAB3310)(1)

Publication using MAB3310

• Mehta A, Zhang L, Boufraqech M, Liu-Chittenden Y, Zhang Y, Patel D, Davis S, Rosenberg A, Ylaya K, Aufforth R, Li Z, Shen M, Kebebew E Inhibition of Survivin with YM155 Induces Durable Tumor Response in Anaplastic Thyroid Cancer. Clin Cancer Res, 2015-05-05;21(18):4123-32. 2015-05-05 [PMID: 25944801] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CLSPN
• Uniprot:
  • Q9HAW4()