CITED4 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: CITED4 Antibody [NBP2-33788] - Immunofluorescent staining of human cell line A549 shows localization to vesicles.

Immunohistochemistry-Paraffin: CITED4 Antibody [NBP2-33788] - Staining of human heart muscle shows moderate membranous positivity in myocytes.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CITED4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CITED4 Antibody - BSA Free (NBP2-33788) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: GGMDAELIDEEALTSLELEL
• Predicted Species: Mouse (90%), Rat (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CITED4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  CITED4 Protein (NBP2-33788PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for CITED4 Antibody - BSA Free

• cbp/p300-interacting transactivator 4
• Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain4,MRG-2
• MRG2
• MSG1-related protein 2
• transcriptional co-activator 4

Background

CITED4 is a member of the CITED family proteins that bind to CBP/p300 transcriptional integrators and functions as a transcriptional coactivator of TFAP2/AP-2. CITED-4 has been proposed as a inhibitor of transactivation for HIF-1 alpha, as it is thought to disrupt HIF-1a interaction with CREBBP.

CITED 4 antibodies are useful tools for research on hypoxic response mechanisms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CITED4
• Uniprot:
  • Q96RK1(Human)