Cited-2 Antibody (JA22)


Western Blot: Cited-2 Antibody (JA22) [NB100-136] - Total protein from human HeLa and MCF7 and mouse 3T3 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in more
Immunocytochemistry/ Immunofluorescence: Cited-2 Antibody (JA22) [NB100-136] - The Cited-2 (JA22) antibody was tested at a 1:250 dilution in HeLa cells against Dylight 488 (Green). Beta-tubulin and nuclei were more
Immunohistochemistry-Paraffin: Cited-2 Antibody (JA22) [NB100-136] - IHC analysis of a formain fixed paraffin embedded (FFPE) tissue section of human breast cancer using Cited-2 antibody at 1:500 dilution. The primary more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
1.0 mg/ml

Cited-2 Antibody (JA22) Summary

Human CITED 2 protein [UniProt# Q99967]
IgG1 Kappa
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 2ug/mL
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This CITED2 (JA22) antibody is useful in Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at 29 kDa. Additional bands may be seen at 36 kDa representing p35srj, Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 19319572) the alternatively spliced version of CITED2, and at 70 kDa (unknown). Does not work well on mouse tissue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
HeLa Whole Cell Lysate
Read Publications using
NB100-136 in the following applications:

  • 1 publication
  • WB
    3 publications

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Protein A or G purified

Alternate Names for Cited-2 Antibody (JA22)

  • Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain2,cbp/p300-interacting transactivator 2
  • Cited2
  • Cited-2
  • MRG-1
  • MRG1melanocyte-specific gene 1-related gene 1
  • MSG1-related gene 1
  • MSG-related protein 1
  • P35srj


CITED2 (Cbp/p300-interacting transactivator 2; also called MSG-related protein 1/ MRG1 or P35srj) is a CREBBP/EP300-interacting protein which is highly conserved in placental mammals with 95% identity shared between human and mouse protein. CITED2 is a transcriptional co-activator that stabilizes the interactions between DNA-binding proteins and the histone acetyltransferases CBP (CREB -binding protein) as well as p300. It is localized in the nucleus and can be induced upon hypoxic trigger wherein it functions as a negative regulator of HIF1 mediated signaling by competing with HIF1alpha for binding to CBP/p300. Additionally, CITED2 gets induced by several biological stimuli such as cytokines, serum and LPS in different cell type. CITED2 interacts with a diverse array of proteins including SMAD2, SMAD3, LHX1/2, ESR1/2, WT1, EP300, PPARA, TFAP2A, TFAP2B, TFAP2C, HNF4A etc and exerts versatile biological activities. CITED2 is a critical transcriptional cofactor involved in multiple organ development and mice lacking CITED2 die in utero with cardiac and aortic arch malformations, adrenal gland agenesis, small cranial and dorsal root ganglia, exencephaly, and neural crest and left-right patterning defects. Defects in CITED2 have been linked to ventricular septal defect type 2 (VSD2) and atrial septal defect type 8 (ASD8).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cited-2 Antibody (NB100-136)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-P, WB.

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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB100-136 Applications Species
Lau WM, Doucet M, Huang D et al. CITED2 modulates estrogen receptor transcriptional activity in breast cancer cells. Biochem Biophys Res Commun 2013 Jun 27 [PMID: 23811274] (WB, Human) WB Human
Goncalves AM, de Sanctis D, McSweeney SM. Structural and functional insights into DR2231 protein, the MazG-like nucleoside triphosphate pyrophosphohydrolase from Deinococcus radiodurans. J Biol Chem 2011 Sep 2 [PMID: 21733847]
Sengupta A, Molkentin JD, Paik JH. FoxO transcription factors promote cardiomyocyte survival upon induction of oxidative stress. J Biol Chem. 2010 Dec 15. [PMID: 21159781]
Yang Y, Dahly-Vernon AJ, Blomme EA et al. Liver transcriptomic changes associated with ritonavir-induced hyperlipidemia in sensitive and resistant strains of rats. Vet J 2010 Jul [PMID: 20547082]
Lau WM, Weber KL, Doucet M et al. Identification of prospective factors promoting osteotropism in breast cancer: a potential role for CITED2. Int J Cancer 2010 Feb 15 [PMID: 19642106] (WB, Mouse) WB Mouse
Haase M, Ansurudeen I, Schinner S. et al et al. Evidence for the involvement of endothelial cell products in adrenal CITED2 expression. Cell Tissue Res 2009 May [PMID: 19319572] (IHC-P, WB, Human) IHC-P, WB Human
Gonzalez YR, Zhang Y, Behzadpoor D, Cregan S, Bamforth S, Slack RS, Park DS. CITED2 signals through peroxisome proliferator-activated receptor-gamma to regulate death of cortical neurons after DNA damage. J Neurosci;28(21):5559-69. 2008 May 21. [PMID: 18495890]
Warnecke C, Weidemann A, Volke M, Schietke R, Wu X, Knaup KX, Hackenbeck T, Bernhardt W, Willam C, Eckardt KU, Wiesener MS. The specific contribution of hypoxia-inducible factor-2alpha to hypoxic gene expression in vitro is limited and modulated by cell type-specific and exogenous factors. Exp Cell Res;314(10):2016-27. 2008 Jun 10. [PMID: 18420194]
Haase, M et al. CITED2 is expressed in human adrenocortical cells and regulated by basic fibroblast growth factor. J Endocrinol;192(2):459-65. 2007 Feb. [PMID: 17283246]
Bamforth, S et al. Cited2 controls left-right patterning and heart development through a Nodal-Pitx2c pathway. Nat Genet;36(11):1189-96. 2004 Nov. [PMID: 15475956]
Show All 12 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cited-2 Antibody (NB100-136) (0)

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Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-136AF350
Alexa Fluor 405 NB100-136AF405
Alexa Fluor 488 NB100-136AF488
Alexa Fluor 532 NB100-136AF532
Alexa Fluor 594 NB100-136AF594
Alexa Fluor 647 NB100-136AF647
Alexa Fluor 700 NB100-136AF700
Alexa Fluor 750 NB100-136AF750
Biotin NB100-136B
DyLight 350 NB100-136UV
DyLight 405 NB100-136V
DyLight 488 NB100-136G
DyLight 550 NB100-136R
DyLight 594 NB100-136DL594
DyLight 650 NB100-136C
DyLight 680 NB100-136FR
DyLight 755 NB100-136IR
FITC NB100-136F
HRP NB100-136H
Janelia Fluor 549 NB100-136JF549
Janelia Fluor 646 NB100-136JF646

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Gene Symbol CITED2