Cited-2 Antibody (JA22)

Western Blot: Cited-2 Antibody (JA22) [NB100-136] - Total protein from human HeLa and MCF7 and mouse 3T3 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cited in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.

Immunocytochemistry/Immunofluorescence: Cited-2 Antibody (JA22) [NB100-136] - The Cited-2 (JA22) antibody was tested at a 1:250 dilution in HeLa cells against Dylight 488 (Green). Beta-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).

Immunohistochemistry-Paraffin: Cited-2 Antibody (JA22) [NB100-136] - IHC analysis of a formain fixed paraffin embedded (FFPE) tissue section of human breast cancer using Cited-2 antibody at 1:500 dilution. The primary antibody bound to CAIX antigens in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. Staining was localized to the nucleus.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clone: JA22
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Cited-2 Antibody (JA22) Summary

• Immunogen: Human CITED 2 protein [UniProt# Q99967]
• Localization: Nuclear
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CITED2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2ug/mL
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: This CITED2 (JA22) antibody is useful in Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at 29 kDa. Additional bands may be seen at 36 kDa representing p35srj, Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 19319572) the alternatively spliced version of CITED2, and at 70 kDa (unknown). Does not work well on mouse tissue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Cited-2 Antibody (JA22)

• Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain2,cbp/p300-interacting transactivator 2
• Cited2
• Cited-2
• MRG-1
• MRG1melanocyte-specific gene 1-related gene 1
• MSG1-related gene 1
• MSG-related protein 1
• P35srj

Background

CITED2 (Cbp/p300-interacting transactivator 2; also called MSG-related protein 1/ MRG1 or P35srj) is a CREBBP/EP300-interacting protein which is highly conserved in placental mammals with 95% identity shared between human and mouse protein. CITED2 is a transcriptional co-activator that stabilizes the interactions between DNA-binding proteins and the histone acetyltransferases CBP (CREB -binding protein) as well as p300. It is localized in the nucleus and can be induced upon hypoxic trigger wherein it functions as a negative regulator of HIF1 mediated signaling by competing with HIF1alpha for binding to CBP/p300. Additionally, CITED2 gets induced by several biological stimuli such as cytokines, serum and LPS in different cell type. CITED2 interacts with a diverse array of proteins including SMAD2, SMAD3, LHX1/2, ESR1/2, WT1, EP300, PPARA, TFAP2A, TFAP2B, TFAP2C, HNF4A etc and exerts versatile biological activities. CITED2 is a critical transcriptional cofactor involved in multiple organ development and mice lacking CITED2 die in utero with cardiac and aortic arch malformations, adrenal gland agenesis, small cranial and dorsal root ganglia, exencephaly, and neural crest and left-right patterning defects. Defects in CITED2 have been linked to ventricular septal defect type 2 (VSD2) and atrial septal defect type 8 (ASD8).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cited-2 Antibody (NB100-136)(12)

Publications using NB100-136

• Lau WM, Doucet M, Huang D et al. CITED2 modulates estrogen receptor transcriptional activity in breast cancer cells. Biochem Biophys Res Commun 2013 Jun 27 [PMID: 23811274] (WB, Human)
• Goncalves AM, de Sanctis D, McSweeney SM. Structural and functional insights into DR2231 protein, the MazG-like nucleoside triphosphate pyrophosphohydrolase from Deinococcus radiodurans. J Biol Chem 2011 Sep 2 [PMID: 21733847]
• Sengupta A, Molkentin JD, Paik JH. FoxO transcription factors promote cardiomyocyte survival upon induction of oxidative stress. J Biol Chem. 2010 Dec 15. [PMID: 21159781]
• Yang Y, Dahly-Vernon AJ, Blomme EA et al. Liver transcriptomic changes associated with ritonavir-induced hyperlipidemia in sensitive and resistant strains of rats. Vet J 2010 Jul [PMID: 20547082]
• Lau WM, Weber KL, Doucet M et al. Identification of prospective factors promoting osteotropism in breast cancer: a potential role for CITED2. Int J Cancer 2010 Feb 15 [PMID: 19642106] (WB, Mouse)
• Haase M, Ansurudeen I, Schinner S. et al et al. Evidence for the involvement of endothelial cell products in adrenal CITED2 expression. Cell Tissue Res 2009 May [PMID: 19319572] (IHC-P, WB, Human)
• Gonzalez YR, Zhang Y, Behzadpoor D, Cregan S, Bamforth S, Slack RS, Park DS. CITED2 signals through peroxisome proliferator-activated receptor-gamma to regulate death of cortical neurons after DNA damage. J Neurosci;28(21):5559-69. 2008 May 21. [PMID: 18495890]
• Warnecke C, Weidemann A, Volke M, Schietke R, Wu X, Knaup KX, Hackenbeck T, Bernhardt W, Willam C, Eckardt KU, Wiesener MS. The specific contribution of hypoxia-inducible factor-2alpha to hypoxic gene expression in vitro is limited and modulated by cell type-specific and exogenous factors. Exp Cell Res;314(10):2016-27. 2008 Jun 10. [PMID: 18420194]
• Haase, M et al. CITED2 is expressed in human adrenocortical cells and regulated by basic fibroblast growth factor. J Endocrinol;192(2):459-65. 2007 Feb. [PMID: 17283246]
• Bamforth, S et al. Cited2 controls left-right patterning and heart development through a Nodal-Pitx2c pathway. Nat Genet;36(11):1189-96. 2004 Nov. [PMID: 15475956]
• Tien, E et al. Identification of the CREB-binding protein/p300-interacting protein CITED2 as a peroxisome proliferator-activated receptor alpha coregulator. J Biol Chem;279(23):24053-63. 2004 Jun 4. [PMID: 15051727]
• Bhattacharya, S et al. Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1. Genes Dev;13(1):64-75. 1999 Jan 1. [PMID: 9887100]

Bioinformatics

• Gene Symbol: CITED2
• Entrez:
  • 10370(Human)
• Uniprot:
  • Q99967(Human)