CISH/CIS-1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: CISH/CIS-1 Antibody [NBP2-38474] - Staining of human cell line U-2 OS shows localization to microtubules & cytokinetic bridge. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: CISH/CIS-1 Antibody [NBP2-38474] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules and cells in glomeruli.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CISH/CIS-1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CISH/CIS-1 Antibody - BSA Free (NBP2-38474) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: APSLELPKPVMQPLPAGAFLEEVAEGTPAQTESEPKVLDPEEDLLCIAKTFSYLRESGWYWGSITASEARQHLQKMPEGTFLVRDSTHPSYLFTLSVKTTRGPTNVRIEYADSSFRLDSNCLSR
• Predicted Species: Mouse (91%), Rat (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CISH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  CISH/CIS-1 Protein (NBP2-38474PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CISH/CIS-1 Antibody - BSA Free

• CIS-1
• CIS-1cytokine-inducible SH2-containing protein
• CIScytokine-inducible inhibitor of signaling type 1B
• CISH
• cytokine inducible SH2-containing protein
• G18Protein G18
• SOCS
• Suppressor of cytokine signaling

Background

CISH is encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CISH
• Uniprot:
  • Q9NSE2()