CIDEC Antibody

Western Blot: CIDEC Antibody [NBP2-15903] - Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 12% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: CIDEC Antibody [NBP2-15903] - Analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.

CIDEC antibody detects CIDEC protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CIDEC protein stained by CIDEC antibody (NBP2-15903) diluted at 1:500.Blue: Hoechst 33343 staining.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CIDEC Antibody Summary

• Description: Novus Biologicals Rabbit CIDEC Antibody (NBP2-15903) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human CIDEC. The exact sequence is proprietary.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CIDEC
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Pig (80%), Sheep (84%), Bovine (83%)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for CIDEC Antibody

• cell death activator CIDE-3
• cell death-inducing DFFA-like effector c
• Cell death-inducing DFFA-like effector protein C
• CIDE-3
• CIDEC
• CIDEX
• Fat-specific protein FSP27 homolog
• FLJ20871
• FSP27
• FSP27fat specific protein 27

Background

DNA fragmentation factor (DFF) induces the fragmentation of DNA associated with apoptosis. A novel family of cell death-inducing DFF45 (MIM 601882)-like effectors (CIDEs), including CIDEC, can also promote apoptosis (Liang et al., 2003 [PubMed 12429024]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CIDEC
• Uniprot:
  • Q96AQ7(Human)