CIDEC Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: CIDEC Antibody [NBP2-15902] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: CIDEC Antibody [NBP2-15902] - BT474 xenograft, using CIDEC antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: CIDEC Antibody [NBP2-15902] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CIDEC antibody [N1C3] (NBP2-15902) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CIDEC Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CIDEC Antibody - BSA Free (NBP2-15902) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-CIDEC Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human CIDEC Recombinant protein.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CIDEC
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Porcine (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for CIDEC Antibody - BSA Free

• cell death activator CIDE-3
• cell death-inducing DFFA-like effector c
• Cell death-inducing DFFA-like effector protein C
• CIDE-3
• CIDEC
• CIDEX
• Fat-specific protein FSP27 homolog
• FLJ20871
• FSP27
• FSP27fat specific protein 27

Background

DNA fragmentation factor (DFF) induces the fragmentation of DNA associated with apoptosis. A novel family of cell death-inducing DFF45 (MIM 601882)-like effectors (CIDEs), including CIDEC, can also promote apoptosis (Liang et al., 2003 [PubMed 12429024]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for CIDEC Antibody (NBP2-15902)(1)

Publication using NBP2-15902

• Dabir S, Kluge A, McColl K et al. PIAS3 activates the intrinsic apoptotic pathway in non-small cell lung cancer cells independent of p53 status Int J Cancer 2013-08-21 [PMID: 23959540] (WB, Human)
  
  Details:
  Citation using the Non-Recombinant Monoclonal format of this antibody.

Bioinformatics

• Gene Symbol: CIDEC
• Uniprot:
  • Q96AQ7(Human)