Chymase/CMA1/Mast Cell Chymase Antibody (CC1)

Immunohistochemistry: Chymase/CMA1/Mast Cell Chymase Antibody (CC1) [NB100-693] - Staining of a human tonsil cross section

Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (CC1) [NB100-693] - Formalin fixed paraffin embedded human tonsil stained with Mast Cell Chymase antibody.

• Reactivity: Hu
• Applications: IHC
• Clone: CC1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Chymase/CMA1/Mast Cell Chymase Antibody (CC1) Summary

• Description: Novus Biologicals Mouse Chymase/CMA1/Mast Cell Chymase Antibody (CC1) (NB100-693) is a monoclonal antibody validated for use in IHC. Anti-Chymase/CMA1/Mast Cell Chymase Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: BALB/C mice were injected with a purified human skin chymase.
• Localization: Cytoplasm
• Specificity: This reacts with mast cells distributed in skin, synovium, lung and heart.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CMA1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
• Application Notes: IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.
• Control:

  Human Tonsil Whole Tissue Lysate (Adult Whole Normal)
  Chymase/CMA1/Mast Cell Chymase Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4), 0.2% BSA, Tween-20
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Chymase/CMA1/Mast Cell Chymase Antibody (CC1)

• Alpha-chymase
• chymase 1 preproprotein transcript E
• chymase 1 preproprotein transcript I
• chymase 1, mast cell
• Chymase
• chymase, heart
• chymase, mast cell
• CMA1
• CYH
• CYM
• EC 3.4.21
• EC 3.4.21.39
• Mast cell protease I
• MCP3P
• Mcpt5
• MCT1
• MGC119890
• MGC119891

Background

Mast cells contain a number of preformed chemicals mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Chymase is considered to be an important marker of mast cells as well as an important mediator of inflammation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Chymase/CMA1/Mast Cell Chymase Antibody (NB100-693)(2)

Publications using NB100-693

• Kallionpaa Ra, Ahramo K, Martikkala E Et Al. Mast Cells in Human Cutaneous Neurofibromas: Density, Subtypes, and Association with Clinical Features in Neurofibromatosis 1 Dermatology (Basel, Switzerland) 2021-07-08 [PMID: 34237737] (IF/IHC)
• Hsieh, FH et al. Human airway epithelial cell determinants of survival functional phenotype for primary human mast cells. PNAS. 102:14380-14385. 2005-01-01 [PMID: 16186496]

FAQs for Chymase/CMA1/Mast Cell Chymase Antibody (NB100-693). (Showing 1 - 2 of 2 FAQs).

1. Could you let me know the concentration of sodium azide?
   • It is supplied in PBS with 0.05% sodium azide.
2. May I know the concentration of the sodium azide contained in Cat. #NB100-693 (anti-Mast Cell Chymase antibody), lot #A936? We have purchased this recently and the user needs to know how much sodium azide is present.
   • I can confirm that the sodium azide concentration in this antibody is 0.05%.If you require any further information, please do not hesitate to get in touch.

Bioinformatics

• Gene Symbol: CMA1
• Entrez:
  • 1215(Human)
• Uniprot:
  • P23946()