Chromogranin A Antibody - BSA Free

Immunohistochemistry-Paraffin: Chromogranin A Antibody [NB120-15160] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse pancreas using Chromogranin A antibody at a dilution of 1:500. The signal was developed using HRP-conjugated anti-rabbit secondary and DAB reagent which followed counterstaining of the section with hematoxylin. The antibody generated a strong staining in Islets of Langerhans. Also, in the periphery of the cells, the pattern of staining was punctate representing the vesicles with Chromogranin A. Cells of lobules and acini showed a weak cytoplasmic and nuclear staining which is potentially the secreted pool of Chromogranin A.

Immunohistochemistry: Chromogranin A Antibody [NB120-15160] - IHC-P analysis fo human pancreas tissue showing specific staining of Chromogranin A in the Islets of Langerhans (dark brown staining).

Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody [NB120-15160] - The frequency of chromogranin A positive hormone-negative (CPHN) cells decreases with age. Representative pancreatic sections from fetal (A) and infant-child (B-D) cases immunostained for Endocrine cocktail (insulin, glucagon, somatostatin, pancreatic polypeptide, and ghrelin) (white), chromogranin A (green), and DAPI (blue). Yellow arrows show CPHN cells. Scale bars, 50 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fendo.2018.00791/full), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody [NB120-15160] - SH-SY5Y cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Chromogranin A at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody [NB120-15160] - Mouse pancreas cryosections stained with insulin (DAKO, Green) and Chromogranin A (NB120-15160; 1:500; White)

Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody [NB120-15160] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Chromogranin A Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160] - ChromograninA positive hormone-negative (CPHN) cells do not replicate during fetal & infant life. Representative pancreatic sections from fetal (A) & infant (B) donors immunostained for Endocrine cocktail (insulin, glucagon, somatostatin, pancreatic polypeptide, & ghrelin) (white), chromograninA (green), Ki67 (red), & DAPI (blue). Yellow arrows indicate CPHN cells. CPHN cells were rarely positive for Ki67 staining in both fetal & infant groups; no detectable difference was found in the frequency of replicative CPHN cells between fetal & infant pancreatic sections. Scale bars: 100 μm for low power & 50 μm for high magnification images. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30687234), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160] - ChromograninA positive hormone-negative (CPHN) cells express the beta-cell differentiation transcription factor NKX2.2 in both fetuses & infants. Representative pancreatic sections from fetal (A) & infant (B) donors immunostained for Endocrine cocktail (insulin, glucagon, somatostatin, pancreatic polypeptide, & ghrelin) (white), chromograninA (green), the transcription factor NKX2.2 (red) & DAPI (blue). Yellow arrows indicate CPHN cells. Scale bars: 100 μm for low power & 25 μm for high magnification images. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30687234), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160] - ChromograninA positive hormone-negative (CPHN) cells express the endocrine differentiation transcription factor NKX6.1 in both fetal & infant pancreas. Representative pancreatic sections from fetal (A) & infant (B) donors immunostained for Endocrine cocktail (insulin, glucagon, somatostatin, pancreatic polypeptide, & ghrelin) (white), chromograninA (green), the transcription factor NKX6.1 (red) & DAPI (blue). Yellow arrows indicate CPHN cells. Scale bars: 100 μm for low power & 25 μm for high magnification images. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30687234), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Chromogranin A Antibody - BSA Free Summary

• Immunogen: Recombinant full length protein (Human).
• Localization: Cytoplasmic, Vesicles, Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHGA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500. Use reported in scientific literature (PMID 22114116)
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot reported in scientific literature (PMID 32818433)
• Application Notes: IHC-P: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Note: Optimal dilutions/concentrations should be determined by the end user.
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Chromogranin A Overexpression Lysate

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27472443).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Chromogranin A Antibody - BSA Free

• betagranin (N-terminal fragment of chromogranin A)
• Betagranin
• Catestatin
• CGA
• CHGA
• Chromofungin
• chromogranin A (parathyroid secretory protein 1)
• Chromogranin A
• chromogranin-A
• Pancreastatin
• Parastatin
• Parathyroid Secretory Protein 1
• Pituitary Secretory Protein I
• SP-I

Background

Chromogranin A (a protein of 439 amino acids which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Chromogranin A Antibody (NB120-15160)(43)

Publications using NB120-15160

• Norris N, Yau B, Famularo C et Al. Optimized Proteomic Analysis of Insulin Granules From MIN6 Cells Identifies Scamp3, a Novel Regulator of Insulin Secretion and Content Diabetes 2024-12-01 [PMID: 39320956]
• Mathijs P Verhagen, Rosalie Joosten, Mark Schmitt, Niko Välimäki, Andrea Sacchetti, Kristiina Rajamäki, Jiahn Choi, Paola Procopio, Sara Silva, Berdine van der Steen, Thierry P P van den Bosch, Danielle Seinstra, Annemarie C de Vries, Michail Doukas, Leonard H Augenlicht, Lauri A Aaltonen, Riccardo Fodde Non-stem cell lineages as an alternative origin of intestinal tumorigenesis in the context of inflammation. Nature genetics 2024-06-20 [PMID: 38902475]
• Finlay JB, Ireland AS, Hawgood SB et al. Olfactory neuroblastoma mimics molecular heterogeneity and lineage trajectories of small-cell lung cancer. Cancer cell 2024-06-11 [PMID: 38788720]
• Aleksandra Aizenshtadt, Chencheng Wang, Shadab Abadpour, Pedro Duarte Menezes, Ingrid Wilhelmsen, Andrea Dalmao-Fernandez, Justyna Stokowiec, Alexey Golovin, Mads Johnsen, Thomas M D Combriat, Hanne Røberg-Larsen, Nikolaj Gadegaard, Hanne Scholz, Mathias Busek, Stephan J K Krauss Pump-Less, Recirculating Organ-On-Chip (rOoC) Platform To Model The Metabolic Crosstalk Between Islets and Liver. Advanced healthcare materials 2024-01-14 [PMID: 38221504]
• Patel S, Becker E, Ploix C et Al. Gut Microbiota Is Associated with Onset and Severity of Type 1 Diabetes in Nonobese Diabetic Mice Treated with Anti-PD-1 Immunohorizons 2023-12-27 [PMID: 38147032]
• Verhagen MP, Joosten R, Schmitt M et al. The origin of intestinal cancer in the context of inflammation bioRxiv : the preprint server for biology 2023-11-01 [PMID: 37873142] (IHC-P, Mouse)
• Tixi W, Maldonado M, Chang YT et al. Coordination between ECM and cell-cell adhesion regulates the development of islet aggregation, architecture, and functional maturation eLife 2023-08-23 [PMID: 37610090]
• Hu X, White K, Olroyd AG et al. Hypoimmune induced pluripotent stem cells survive long term in fully immunocompetent, allogeneic rhesus macaques Nature biotechnology 2023-05-08 [PMID: 37156915] (ICC/IF, Human)
  
  Details:
  Dilution: 1:20
• Quijano JC, Wedeken L, Ortiz JA et Al. Methylcellulose colony assay and single-cell micro-manipulation reveal progenitor-like cells in adult human pancreatic ducts Stem Cell Reports 2023-03-02 [PMID: 36868230]
• Doornebal EJ, Harris N, Riva A et al. Human Immunocompetent Model of Neuroendocrine Liver Metastases Recapitulates Patient-Specific Tumour Microenvironment Front Endocrinol (Lausanne) 2022-07-13 [PMID: 35909511]
• Rosado-Olivieri E In Vitro Modeling of Human beta Cell Differentiation, Regeneration and Function Thesis (IF/IHC, Human)
• Wong AC, Devason AS, Umana IC et al. Serotonin reduction in post-acute sequelae of viral infection Cell 2023-10-26 [PMID: 37848036] (IF/ICC, Human)
• Co JY, Klein JA, Kang S, Homan KA Suspended hydrogel culture as a method to scale up intestinal organoids Scientific reports 2023-06-27 [PMID: 37369732] (Immunohistochemistry, Human)
• Butler W, Xu L, Zhou Y et al. Oncofetal protein glypican-3 is a biomarker and critical regulator of function for neuroendocrine cells in prostate cancer The Journal of pathology 2023-02-08 [PMID: 36752189] (IHC, Human)
• Fodde R, Verhagen M, Joosten R et al. Paneth cells as the origin of intestinal cancer in the context of inflammation Research square 2023-01-19 [PMID: 36711533] (IHC-P, Mouse)
• J Yun, S Hansen, O Morris, DT Madden, CP Libeu, AJ Kumar, C Wehrfritz, AH Nile, Y Zhang, L Zhou, Y Liang, Z Modrusan, MB Chen, CC Overall, D Garfield, J Campisi, B Schilling, RN Hannoush, H Jasper Senescent cells perturb intestinal stem cell differentiation through Ptk7 induced noncanonical Wnt and YAP signaling Nature Communications, 2023-01-11;14(1):156. 2023-01-11 [PMID: 36631445]
• HAfliger J, Schwarzfischer M, Atrott K et al. Glycoprotein (GP)96 Is Essential for Maintaining Intestinal Epithelial Architecture by Supporting Its Self-Renewal Capacity Cellular and molecular gastroenterology and hepatology 2022-12-11 [PMID: 36516930] (IHC-P, Mouse)
• Parchure A, Tian M, Stalder D et al. Liquid-liquid phase separation facilitates the biogenesis of secretory storage granules The Journal of cell biology 2022-12-05 [PMID: 36173346] (Immunocytochemistry/ Immunofluorescence, Rat)
• Kopacz A, Kloska D, Klimczyk D et al. Nrf2 Transcriptional Activity Governs Intestine Development International journal of molecular sciences 2022-05-31 [PMID: 35682851] (IHC-Fr, Mouse)
• Rosado-Olivieri, EA;Anderson, K;Kenty, JH;Melton, DA; YAP inhibition enhances the differentiation of functional stem cell-derived insulin-producing beta cells Nat Commun 2019-04-01 [PMID: 30931946] (IF/IHC, Human)
• Shayeganpour K Creation of a Humanized Mouse Model to Study Islet Graft Rejection Thesis 2022-01-01 (IHC, Human)
• Sriha J, Louis-Brennetot C, Pierre-Eugene C et al. BET and CDK Inhibition Reveal Differences in the Proliferation Control of Sympathetic Ganglion Neuroblasts and Adrenal Chromaffin Cells Cancers 2022-06-01 [PMID: 35681734] (ICC/IF, Mouse)
• Pandey U, Aich P Postnatal intestinal mucosa and gut microbial composition develop hand in hand: A mouse study Biomedical journal 2022-03-16 [PMID: 35306225] (IHC-Fr, Mouse)
• Reck J, Beuret N, Demirci E et al. Small disulfide loops in peptide hormones mediate self-aggregation and secretory granule sorting Life Science Alliance 2022-05-01 [PMID: 35086936]
• Kuo W, Zuo L, Odenwald M Et al. The tight junction protein ZO-1 is dispensable for barrier function but critical for effective mucosal repair Gastroenterology 2021-08-01 [PMID: 34478742]
• Wilson S, Mayo M, Melim T et al. Optimized Culture Conditions for Improved Growth and Functional Differentiation of Mouse and Human Colon Organoids Frontiers in Immunology 2021-02-12 [PMID: 33643277] (IF/IHC, Mouse)
• Lin L, Petralia RS, Lake R et al. A novel structure associated with aging is augmented in the DPP6-KO mouse brain Acta Neuropathol Commun 2020-11-23 [PMID: 33225987] (IF/IHC, Mouse)
  
  Details:
  DPP6-KO and wild type mice hippocampi were analyzed via immunohistochemistry
• Miao Y, Ha A, de Lau W et al. Next-Generation Surrogate Wnts Support Organoid Growth and Deconvolute Frizzled Pleiotropy In Vivo Cell Stem Cell 2020-08-14 [PMID: 32818433] (WB, Human)
• Cohen MA, Zhang S, Sengupta S et al. Formation of Human Neuroblastoma in Mouse-Human Neural Crest Chimeras Cell Stem Cell 2020-02-27 [PMID: 32142683] (IF/IHC, Mouse)
• Muthupalani S, Ge Z, Joy J et al. Muc5ac null mice are predisposed to spontaneous gastric antro-pyloric hyperplasia and adenomas coupled with attenuated H. pylori-induced corpus mucous metaplasia Lab. Invest. 2019-08-09 [PMID: 31399638] (IHC-P, Mouse)
• Moin ASM, Montemurro C, Zeng K et al. Characterization of Non-hormone Expressing Endocrine Cells in Fetal and Infant Human Pancreas. Front Endocrinol (Lausanne) 2018-01-09 [PMID: 30687234] (ICC/IF, Human)
• Schmitt M, Schewe M, Sacchetti A et al. Paneth Cells Respond to Inflammation and Contribute to Tissue Regeneration by Acquiring Stem-like Features through SCF/c-Kit Signaling Cell Rep 2018-08-28 [PMID: 30157426] (ICC/IF, Mouse)
• Md Moin AS, Cory M, Ong A et al. Pancreatic Nonhormone Expressing Endocrine Cells in Children With Type 1 Diabetes J Endocr Soc 2017-05-01 [PMID: 28782056] (IHC-P, Human)
• Ana E Carvajal, José M Serrano-Morales, María D Vázquez-Carretero, Pablo García-Miranda, María L Calonge, María J Peral, Anunciación A Ilundain Reelin protects from colon pathology by maintaining the intestinal barrier integrity and repressing tumorigenic genes. Biochimica et biophysica acta. Molecular basis of disease 2018-11-27 [PMID: 28572005]
• Tuysuz N, van Bloois L, van den Brink S et al. Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells. Nat Commun. 2017-03-06 [PMID: 28262686] (IHC-P, Human)
• Saleh Md Moin A, Dhawan S, Cory M et al. Increased frequency of hormone negative and polyhormonal endocrine cells in lean individuals with type 2 diabetes. J. Clin. Endocrinol. Metab. 2016-07-29 [PMID: 27472443] (IF/IHC, Rat)
• Md Moin AS, Dhawan S, Shieh C et al. Increased hormone-negative endocrine cells in the pancreas in type 1 diabetes. J. Clin. Endocrinol. Metab. 2016-06-14 [PMID: 27300574] (IHC-P)
• Helman A, Klochendler A, Azazmeh N, Gabai Y. p16Ink4a-induced senescence of pancreatic beta cells enhances insulin secretion. Nature medicine. 2016-03-07 [PMID: 26950362] (IHC-P, Mouse)
• Butler AE, Dhawan S, Hoang J et al. Beta-Cell Deficit in Obese Type 2 Diabetes, a Minor Role of Beta-Cell Dedifferentiation and Degranulation J Clin Endocrinol Metab. 2015-12-22 [PMID: 26700560] (IHC-P, Human)
• Hubbard GK, Mutton LN, Khalili M et al. Combined MYC Activation and Pten Loss Are Sufficient to Create Genomic Instability and Lethal Metastatic Prostate Cancer. Cancer Res 2016-01-15 [PMID: 26554830]
• Kwon SY, Bae YK, Gu MJ et al. Neuroendocrine differentiation correlates with hormone receptor expression and decreased survival in patients with invasive breast carcinoma. Histopathology. 2013-10-09 [PMID: 24117859] (IHC-P, Human)
• Park JK, Cho EJ, Ryu JK et al. Natural History and Malignant Risk Factors of Solid Pseudopapillary Tumors of the Pancreas Postgrad Med. 2013-03-01 [PMID: 23816775] (IHC-P, Human)
• Polyak S, Mach A, Porvasnik S et al. Identification of Adeno-Associated Viral Vectors Suitable for Intestinal Gene Delivery and Modulation of Experimental Colitis. American Journal of Physiology. Gastrointestinal and Liver Physiology. [PMID: 22114116]

Reviews for Chromogranin A Antibody (NB120-15160) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Jason Spaeth IHC-Fr Mouse 11/29/2016

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Pancreas tissue (cryosection)
• Species: Mouse

reviewed by: Verified Customer IF Mouse 05/07/2012

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse small intestine and colon
• Species: Mouse

FAQs for Chromogranin A Antibody (NB120-15160). (Showing 1 - 1 of 1 FAQs).

1. I have following question about antibodies against Chromogranin A. Your products list the species but canine is not included. Does it mean that the products cannot be used on canine tissues?
   • When we do not list a species, it means we have not tested an antibody to work with those samples. If we test an antibody and find that it does not work, we will list the species with a minus sign. Unfortunately, we haven't tried these abs on canine tissues and they may work, but we cannot guarantee it. If you would like to purchase one to try, you will be eligible for the Innovators Reward Program.

Bioinformatics

• Gene Symbol: CHGA
• Entrez:
  • 1113(Human)
• Uniprot:
  • P10645()