Choline Acetyltransferase/ChAT Antibody (334008)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
334008
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Choline Acetyltransferase/ChAT Antibody (334008) Summary

Immunogen
E. coli-derived recombinant human Choline Acetyltransferase/ChAT isoform 1
Ala2-Pro630
Accession # NP_066266
Specificity
Detects human Choline Acetyltransferase/ChAT in direct ELISAs and Western blots. Recognizes both ChAT isoform 1 (ChAT-69) and isoform 2 (ChAT-82).
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CHAT
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Control
Choline Acetyltransferase/ChAT Knockout HeLa Cell Lysate
Publications
Read Publication using
MAB3447 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Choline Acetyltransferase/ChAT Antibody (334008)

  • acetyl CoA:choline O-acetyltransferase
  • CHAT
  • CHOACTase
  • Choline acetylase
  • Choline Acetyltransferase
  • choline O-acetyltransferase
  • CMS1A
  • CMS1A2
  • EC 2.3.1
  • EC 2.3.1.6

Background

ChAT catalyzes the production of the neurotransmitter acetylcholine (Ach), which is required for cholinogenic neuron communication. It serves as a marker for functional cholinogenic neurons in the central and peripheral nervous systems. At least six ChAT mRNAs encoding 69 kDa, 82 kDa and 74 kDa ChAT proteins have been identified. Compared to the 82 kDa form, 69 kDa ChAT lacks the N‑terminal 118 amino acid extension containing a nuclear localization signal. As a result the 69 kDa ChAT is primarily localized to the cytoplasm. Human 69 kDa ChAT shares 86% amino acid sequence identity with the mouse or rat ChAT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Choline Acetyltransferase/ChAT Antibody (MAB3447)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA Capture.


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ELISA Capture
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Choline Acetyltransferase/ChAT Antibody (MAB3447) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for Choline Acetyltransferase/ChAT Antibody (MAB3447)

Discover related pathways, diseases and genes to Choline Acetyltransferase/ChAT Antibody (MAB3447). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Choline Acetyltransferase/ChAT.

Choline Acetyltransferase (ChAT) – a useful Immunohistochemical marker for morphological studies of neurons
Choline Acetyltransferase (ChAT) is the enzyme that is responsible for biosynthesis of the neurotransmitter acetylcholine. The majority of acetylcholine is synthesized locally at nerve terminals where ChAT catalyzes the transfer of an acetyl group...  Read full blog post.

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Bioinformatics

Gene Symbol CHAT
Uniprot