Choline Acetyltransferase/ChAT Antibody (334008) [Janelia Fluor® 525]

• Reactivity: Hu
• Applications: WB
• Clone: 334008
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 525

Choline Acetyltransferase/ChAT Antibody (334008) [Janelia Fluor® 525] Summary

• Immunogen: E. coli-derived recombinant human Choline Acetyltransferase/ChAT isoform 1
  Ala2-Pro630
  Accession # NP_066266
• Specificity: Detects human Choline Acetyltransferase/ChAT in direct ELISAs and Western blots. Recognizes both ChAT isoform 1 (ChAT-69) and isoform 2 (ChAT-82).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHAT
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Choline Acetyltransferase/ChAT Antibody (334008) [Janelia Fluor® 525]

• acetyl CoA:choline O-acetyltransferase
• CHAT
• CHOACTase
• Choline acetylase
• Choline Acetyltransferase
• choline O-acetyltransferase
• CMS1A
• CMS1A2
• EC 2.3.1
• EC 2.3.1.6

Background

Note: This is the same product as NB300-178. FUNCTION: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine. SIMILARITY: Belongs to the carnitine/choline acetyltransferase family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHAT