Chk2 [p Ser516] Antibody - Azide and BSA Free

Western Blot: Chk2 [p Ser516] Antibody [NBP3-16900] - Western blot analysis of extracts of NIH/3T3 cells, using Chk2 pAb (NBP3-16900) at 1:1000 dilution or Chk2 antibody.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Chk2 [p Ser516] Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Chk2 [p Ser516] Antibody - Azide and BSA Free (NBP3-16900) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic phosphorylated peptide around S516 of human Chk2 (NP_009125.1). QPSTS
• Modification: p Ser516
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHEK2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 -1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for Chk2 [p Ser516] Antibody - Azide and BSA Free

• CDS1
• CHEK2
• CHK2 checkpoint homolog (S. pombe)
• Chk2
• EC 2.7.11
• EC 2.7.11.1
• HuCds1
• LFS2
• PP1425
• Rad53
• S.pombe) homolog

Background

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: CHEK2