| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27403 | |||
| BSA Free | NB100-56546SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71688 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44069 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1626-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1358-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB22851-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90024-25ul | |||
![Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Western-Blot-NBP2-27403-img0003.jpg "Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Chk2 (73C175.1.1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the standard format of this product.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-27403-img0006.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Immunohistochemistry-Paraffin-NBP2-27403-img0002.jpg)
![Flow Cytometry: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - An intracellular stain was performed on Hek293 cells with Chk2 [73C175.1.1] Antibody NB100-56546 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550. Image using the standard format of this product.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Flow-Cytometry-NBP2-27403-img0005.jpg)
![Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - analysis of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with Chk2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Western-Blot-NBP2-27403-img0001.jpg)
![Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - analysis of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with Chk2 antibody at 2 ug/ml. Image using the Azide Free form of this antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Western-Blot-NB100-56546-img0003.jpg "Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - analysis of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with Chk2 antibody at 2 ug/ml. Image using the Azide Free form of this antibody.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (73C175.1.1) [NB100-56546] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Chk2 (73C175.1.1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Immunocytochemistry-Immunofluorescence-NB100-56546-img0004.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (73C175.1.1) [NB100-56546] - Staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Immunohistochemistry-Paraffin-NB100-56546-img0002.jpg)
![Flow Cytometry: Chk2 Antibody (73C175.1.1) [NB100-56546] - An intracellular stain was performed on Hek293 cells with Chk2 [73C175.1.1] Antibody NB100-56546 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Flow-Cytometry-NB100-56546-img0006.jpg)
![Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Western-Blot-NB100-56546-img0001.jpg)
![Western Blot: Chk2 Antibody (OTI5C4) [NBP1-47695] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Chk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Chk2.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Western-Blot-NBP1-47695-img0020.jpg "Western Blot: Chk2 Antibody (OTI5C4) [NBP1-47695] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Chk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Chk2.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (OTI5C4) [NBP1-47695] - Chk2 Antibody (5C4) [NBP1-47695] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Chk2.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunocytochemistry-Immunofluorescence-NBP1-47695-img0013.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human pancreas tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0019.jpg)
![Flow Cytometry: Chk2 Antibody (OTI5C4) [NBP1-47695] - Analysis of Jurkat cells, using anti-Chk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Flow-Cytometry-NBP1-47695-img0012.jpg)
![Western Blot: Chk2 Antibody (OTI5C4) [NBP1-47695] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Chk2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Western-Blot-NBP1-47695-img0011.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human breast tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0014.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human colon tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0015.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human Kidney tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0016.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human liver tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0017.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human lung tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0018.jpg)
![Western Blot: Chk2 Antibody (4B7) [H00011200-M01] - CHEK2 monoclonal antibody (M01), clone 4B7. Analysis of CHEK2 expression in HeLa.](http://images.novusbio.com/fullsize/Chk2-Antibody-4B7-Western-Blot-H00011200-M01-img0011.jpg "Western Blot: Chk2 Antibody (4B7) [H00011200-M01] - CHEK2 monoclonal antibody (M01), clone 4B7. Analysis of CHEK2 expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (4B7) [H00011200-M01] - Analysis of monoclonal antibody to CHEK2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-4B7-Immunocytochemistry-Immunofluorescence-H00011200-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (4B7) [H00011200-M01] - Analysis of monoclonal antibody to CHEK2 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-4B7-Immunohistochemistry-Paraffin-H00011200-M01-img0009.jpg)
![Western Blot: Chk2 Antibody (4B7) [H00011200-M01] - CHEK2 monoclonal antibody (M01), clone 4B7 Analysis of CHEK2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Chk2-Antibody-4B7-Western-Blot-H00011200-M01-img0012.jpg)
![Sandwich ELISA: Chk2 Antibody (4B7) [H00011200-M01] - Detection limit for recombinant GST tagged CHEK2 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-4B7-Sandwich-ELISA-H00011200-M01-img0010.jpg)
![Western Blot: Chk2 Antibody (CL9440) [NBP2-88933] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Western-Blot-NBP2-88933-img0008.jpg "Western Blot: Chk2 Antibody (CL9440) [NBP2-88933] - Analysis in human cell line HEL.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of MCF7 cells using the Anti-CHEK2 monoclonal antibody, showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunocytochemistry-Immunofluorescence-NBP2-88933-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Analysis in human testis and cerebral cortex tissues using NBP2-88933 antibody. Corresponding CHEK2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0007.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0002.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0003.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human human embryonal carcinoma of testis shows strong nuclear positivity.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0004.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human testis cancer, seminoma shows strong nuclear positivity.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0005.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (CL9440) [NBP2-88933] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Chk2-Antibody-CL9440-Immunohistochemistry-Paraffin-NBP2-88933-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody [NBP1-90024] - Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.](http://images.novusbio.com/fullsize/Chk2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90024-img0006.jpg "Immunocytochemistry/Immunofluorescence: Chk2 Antibody [NBP1-90024] - Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.")
![Immunohistochemistry-Paraffin: Chk2 Antibody [NBP1-90024] - Staining of human colon shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Chk2-Antibody-Immunohistochemistry-Paraffin-NBP1-90024-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (8F12) [NB100-500] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 1:500 dilution.](http://images.novusbio.com/fullsize/Chk2-Antibody-8F12-Immunocytochemistry-Immunofluorescence-NB100-500-img0003.jpg "Immunocytochemistry/Immunofluorescence: Chk2 Antibody (8F12) [NB100-500] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 1:500 dilution.")
![Western Blot: Chk2 Antibody (8F12) [NB100-500] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk2 antibody [8F12] (NB100-500) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nb100-500_mouse-monoclonal-chk2-antibody-8f12-91020242014681.jpg)
![ELISA Chk2 [p Thr68] [Biotin]](http://images.novusbio.com/fullsize2/dyc1626-2_human-phospho-chk2-t68-duoset-ic-elisa-2-plate-elisa-169202092056.jpg "ELISA Chk2 [p Thr68] [Biotin]")
![Western Blot: Chk2 [p Thr68] Antibody [NBP3-13076] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Thr68) antibody (NBP3-13076) diluted at 1:1000.](http://images.novusbio.com/fullsize/Chk2-[p-Thr68]-Antibody-Western-Blot-NBP3-13076-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk2 [p Thr68] Antibody [NBP3-13076] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Thr68) antibody (NBP3-13076) diluted at 1:1000.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Chk2 [p Thr68] Antibody [NBP3-13076] - Chk2 (phospho Thr68) antibody detects Chk2 (phospho Thr68) protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Chk2 (phospho Thr68) stained by Chk2 (phospho Thr68) antibody (NBP3-13076) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:500. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Chk2-[p-Thr68]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13076-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: Chk2 [p Thr68] Antibody [NBP3-13076] - Wild-type (WT) and Chk2 (phospho Thr68) knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 (phospho Thr68) antibody (NBP3-13076) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Chk2-[p-Thr68]-Antibody-Western-Blot-NBP3-13076-img0003.jpg)