Chk1 [p Ser317] Antibody - BSA Free

Western Blot: Chk1 [p Ser317] Antibody [NBP2-37703] - Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer treated with hydroxyurea (2mM for 24 hours), EPE (100uM for 4 hours), or untreated. Affinity purified rabbit anti-Chk1, Phospho (S317) antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry: Chk1 [p Ser317] Antibody [NBP2-37703] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Chk1, Phospho (S317) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

• Reactivity: Hu
• Applications: WB, IHC, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Chk1 [p Ser317] Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Chk1 [p Ser317] Antibody - BSA Free (NBP2-37703) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody maps to a region between residue Immunogen for A304-673A was a phosphorylated synthetic peptide, which represented a portion of human Checkpoint Kinase 1 (GeneID 1111) around serine 317 according to the numbering given in entry NP_001265.1. of human Checkpoint Kinase 1 using the numbering given in entry NP_001265.1 (GeneID 1111).
• Modification: p Ser317
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHEK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Western Blot 1:2000 - 1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Chk1 [p Ser317] Antibody - BSA Free

• Checkpoint, S. pombe, homolog of, 1
• CHEK1
• CHK1 (checkpoint, S.pombe) homolog
• CHK1 checkpoint homolog (S. pombe)
• Chk1
• CHK1serine/threonine-protein kinase Chk1
• EC 2.7.11
• EC 2.7.11.1

Background

Chk1 is involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can bind to, phosphorylate (at serine 216) and inactivate cdc25c (3,4). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHEK1
• Uniprot:
  • NP_001265.1(Human)