Chk1 [p Ser296] Antibody (SR1961) - BSA Free

Western Blot: Chk1 [p Ser 296] Antibody (SR1961) [NBP3-21549] - Western blot analysis using NBP3-21549 in HEK293 cell lysate Treated with Calyculin.

• Reactivity: Hu
• Applications: WB
• Clone: SR1961
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Chk1 [p Ser296] Antibody (SR1961) - BSA Free Summary

• Description: Novus Biologicals Rabbit Chk1 [p Ser296] Antibody (SR1961) - BSA Free (NBP3-21549) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human phospho-Chk1 [Ser296] (Uniprot #: O14757)
• Modification: p Ser296
• Specificity: Detects endogenous levels of phospho-Chk1 [Ser296]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CHEK1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Chk1 [p Ser296] Antibody (SR1961) - BSA Free

• Checkpoint, S. pombe, homolog of, 1
• CHEK1
• CHK1 (checkpoint, S.pombe) homolog
• CHK1 checkpoint homolog (S. pombe)
• Chk1
• CHK1serine/threonine-protein kinase Chk1
• EC 2.7.11
• EC 2.7.11.1

Background

Chk1 is involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can bind to, phosphorylate (at serine 216) and inactivate cdc25c (3,4). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHEK1