Chk1 Antibody (2G1D5) - Azide and BSA Free

Western Blot: Chk1 Antibody (2G1D5) - Azide and BSA Free [NBP2-80668] - Analysis using Chk1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Chk1 cDNA (2). Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: Chk1 Antibody (2G1D5) - Azide and BSA Free [NBP2-80668] - Chk1 antibody was tested at 1:10 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40X. Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: Chk1 Antibody (2G1D5) - Azide and BSA Free [NBP2-80668] - Analysis of FFPE human lung using Chk1 antibody (clone 2G1D5) at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase

Western Blot: Chk1 Antibody (2G1D5) - Azide and BSA Free [NBP2-80668] - Analysis using anti-Chk1 mouse mAb against A431 (1), Hela (2), NIH/3T3 (3) and K562 (4) cell lysates. Image from the standard format of this antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: 2G1D5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Chk1 Antibody (2G1D5) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Chk1 Antibody (2G1D5) - Azide and BSA Free (NB100-1768) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of Chk1 expressed in E. coli. [UniProt# O14757]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHEK1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Paraffin 1:250
  • Western Blot 1:200 - 1:1000
• Application Notes: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Chk1 Antibody (2G1D5) - Azide and BSA Free

• Checkpoint, S. pombe, homolog of, 1
• CHEK1
• CHK1 (checkpoint, S.pombe) homolog
• CHK1 checkpoint homolog (S. pombe)
• Chk1
• CHK1serine/threonine-protein kinase Chk1
• EC 2.7.11
• EC 2.7.11.1

Background

Chk1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by Chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHEK1