![Western Blot: CDCA7L Antibody (OTI3B5) [NBP2-46198] - Detection of CDCA7L by western blot. Samples: Whole cell lysate from prostate cancer; 40 ug. Antibodies:CDCA7L Antibody [NBP2-46198] at dilutions 1:500, 1:1000, 1:2000. Detection: Chemiluminescence with exposure of 5 seconds. Image from verified customer review.](http://images.novusbio.com/fullsize2/CDCA7L-Antibody-(OTI3B5)-Western-Blot-NBP2-46198-img0010.jpg "Western Blot: CDCA7L Antibody (OTI3B5) [NBP2-46198] - Detection of CDCA7L by western blot. Samples: Whole cell lysate from prostate cancer; 40 ug. Antibodies:CDCA7L Antibody [NBP2-46198] at dilutions 1:500, 1:1000, 1:2000. Detection: Chemiluminescence with exposure of 5 seconds. Image from verified customer review.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | OTI3B5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1-337 of human CDCA7L (NP_061189) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CDCA7L |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | Western blot reported by a verified customer review. |
|
| Theoretical MW | 52 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
| Reviewed Applications |
|
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Greisha Lee Ortiz-Hernandez |
WB | Human | 06/05/2018 |
Summary
|
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-72009AF350 | |
| Alexa Fluor 405 | NBP2-72009AF405 | |
| Alexa Fluor 488 | NBP2-72009AF488 | |
| Alexa Fluor 532 | NBP2-72009AF532 | |
| Alexa Fluor 594 | NBP2-72009AF594 | |
| Alexa Fluor 647 | NBP2-72009AF647 | |
| Alexa Fluor 700 | NBP2-72009AF700 | |
| Alexa Fluor 750 | NBP2-72009AF750 | |
| Biotin | NBP2-72009B | |
| DyLight 350 | NBP2-72009UV | |
| DyLight 405 | NBP2-72009V | |
| DyLight 488 | NBP2-72009G | |
| DyLight 550 | NBP2-72009R | |
| DyLight 594 | NBP2-72009DL594 | |
| DyLight 650 | NBP2-72009C | |
| DyLight 680 | NBP2-72009FR | |
| DyLight 755 | NBP2-72009IR | |
| FITC | NBP2-72009F | |
| HRP | NBP2-72009H | |
| Janelia Fluor 549 | NBP2-72009JF549 | |
| Janelia Fluor 646 | NBP2-72009JF646 |
Diseases for CDCA7L Antibody (NBP2-46198)Discover more about diseases related to CDCA7L Antibody (NBP2-46198).
| Pathways for CDCA7L Antibody (NBP2-46198)View related products by pathway.
|
PTMs for CDCA7L Antibody (NBP2-46198)Learn more about PTMs related to CDCA7L Antibody (NBP2-46198).
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
5 | |
4 | |
3 | |
2 | |
1 |
| Greisha Lee Ortiz-Hernandez 06/05/2018 |
||
| Application: | WB | |
| Species: | Human |
| Gene Symbol | CDCA7L |
![Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/CDCA7L-Antibody-(3B5)-Immunohistochemistry-NBP2-46198-img0007.jpg "Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Western Blot: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDCA7L.](http://images.novusbio.com/fullsize2/CDCA7L Antibody (3B5)-Western Blot-NBP2-46198-img0008.jpg "Western Blot: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDCA7L.")
![Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/CDCA7L-Antibody-(3B5)-Immunohistochemistry-NBP2-46198-img0001.jpg "Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/CDCA7L-Antibody-(3B5)-Immunohistochemistry-NBP2-46198-img0002.jpg "Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/CDCA7L-Antibody-(3B5)-Immunohistochemistry-NBP2-46198-img0003.jpg "Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/CDCA7L-Antibody-(3B5)-Immunohistochemistry-NBP2-46198-img0004.jpg "Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/CDCA7L-Antibody-(3B5)-Immunohistochemistry-NBP2-46198-img0005.jpg "Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/CDCA7L-Antibody-(3B5)-Immunohistochemistry-NBP2-46198-img0006.jpg "Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Western Blot: RRM2 Antibody [NBP1-31661] - Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/images2/RRM2-Antibody-Western-Blot-NBP1-31661-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: RRM2 Antibody [NBP1-31661] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RRM2 protein stained by RRM2 antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/images2/RRM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31661-img0011.jpg)
![Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Mouse tumor tissues were stained with RRM2 antibody at 1:50 dilution. Image submitted by a verified customer review.](http://images.novusbio.com/images2/RRM2 Antibody-Immunohistochemistry-Paraffin-NBP1-31661-img0012.jpg)
![Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of human tonsil (paraffin-embedded sections) with anti-CD82 (C33).](http://images.novusbio.com/images2/CD82-Kai-1-Antibody-(C33)-Immunohistochemistry-Paraffin-NBP2-21792-img0002.jpg)
![Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Analysis (surface staining) of CD82 on human peripheral blood cells with anti-CD82 (C33) PE.](http://images.novusbio.com/images2/CD82-Kai-1-Antibody-(C33)-Flow-Cytometry-NBP2-21792-img0007.jpg)
![Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of CD82 on human peripheral blood cells with anti-CD82 (C33) APC.](http://images.novusbio.com/images2/CD82-Kai-1-Antibody-(C33)-Flow-Cytometry-NBP2-21792-img0001.jpg)
![Immunohistochemistry-Frozen: CD1a Antibody (NA1/34-HLK) [NB100-64852] - Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD1a antibody followed by HISTAR detection system. Medium power](http://images.novusbio.com/images2/CD1a-Antibody-(NA1-34-HLK)-Immunohistochemistry-Frozen-NB100-64852-img0007.jpg)
![Flow Cytometry: CD1a Antibody (NA1/34-HLK) [NB100-64852] - Analysis using the FITC conjugate of NB100-64852. Staining of MOLT 4 cells.](http://images.novusbio.com/images2/CD1a-Antibody-(NA1-34-HLK)-Flow-Cytometry-NB100-64852-img0005.jpg)
![Immunohistochemistry-Frozen: CD1a Antibody (NA1/34-HLK) [NB100-64852] - mmunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD1a antibody followed by HISTAR detection system. Low power](http://images.novusbio.com/images2/CD1a-Antibody-(NA1-34-HLK)-Immunohistochemistry-Frozen-NB100-64852-img0006.jpg)
![Western Blot: IP3R1 Antibody [NBP1-21398] - Whole cell lysate from HeLa cells. Affinity purified rabbit anti-IP3R1 antibody. IP3R1 was also immunoprecipitated by rabbit anti-IP3R1 antibody NBP1-21397.](http://images.novusbio.com/images2/IP3R1-Antibody-Western-Blot-NBP1-21398-img0002.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-21398] - Section of mouse cerebellum. Antibody: Affinity purified rabbit anti- IP3R1 used at a dilution of 1:100 (2ug/ml). Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated used ata dilution of 1:100. Counterstain: DAPI (blue).](http://images.novusbio.com/images2/IP3R1-Antibody-Immunohistochemistry-NBP1-21398-img0006.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-21398] - Section of mouse cerebellum. Antibody: Affinity purified rabbit anti- IP3R1 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/images2/IP3R1-Antibody-Immunohistochemistry-NBP1-21398-img0005.jpg)
![Western Blot: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of hnRNP C1+C2 on different lysates using anti-hnRNP C1+C2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: HepG2](http://images.novusbio.com/images2/hnRNP-C1-+-C2-Antibody-(SN0652)-Western-Blot-NBP2-67656-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in B16-F1 (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/hnRNP C1 + C2 Antibody (SN0652)-Immunocytochemistry-Immunofluorescence-NBP2-67656-img0010.jpg)
![Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of paraffin-embedded mouse skin tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/hnRNP-C1-+-C2-Antibody-(SN0652)-Immunohistochemistry-Paraffin-NBP2-67656-img0008.jpg)
![Western Blot: PMEL17/SILV Antibody [NBP1-69571] - Sample Tissue: Human MCF7 Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/images2/PMEL17-SILV-Antibody-Western-Blot-NBP1-69571-img0006.jpg)
![Immunohistochemistry: PMEL17/SILV Antibody [NBP1-69571] - The inflammatory MITFlow/c-Junhigh cell state correlates with increased myeloid cell infiltration in human melanomas. Immunohistochemical analysis for gp100, CD45 and CD14 in representative cases from the Lund melanoma cohort. Small panels show zoom-in views, for example, to visualize morphology of CD45+/CD14+ tumour-infiltrating immune cells. Scale bars, 50 um. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/ncomms9755) licensed under a CC-BY licence.](http://images.novusbio.com/images2/PMEL17-SILV-Antibody-Immunohistochemistry-NBP1-69571-img0007.jpg)
![Western Blot: PMEL17/SILV Antibody [NBP1-69571] - This Anti-SILV antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 1.25ug/ml.](http://images.novusbio.com/images2/PMEL17-SILV-Antibody-Western-Blot-NBP1-69571-img0003.jpg)
![Western Blot: CD1c/BDCA-1 Antibody (OTI2F4) [NBP2-46123] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD1c.](http://images.novusbio.com/images2/CD1c-BDCA-1-Antibody-(OTI2F4)-Western-Blot-NBP2-46123-img0007.jpg)
![Immunohistochemistry: CD1c/BDCA-1 Antibody (OTI2F4) [NBP2-46123] - Analysis of Human endometrium tissue.](http://images.novusbio.com/images2/CD1c-BDCA-1-Antibody-(OTI2F4)-Immunohistochemistry-NBP2-46123-img0006.jpg)
![Western Blot: CD1c/BDCA-1 Antibody (OTI2F4) [NBP2-46123] - Analysis of Jurkat extracts (10ug).](http://images.novusbio.com/images2/CD1c-BDCA-1-Antibody-(OTI2F4)-Western-Blot-NBP2-46123-img0008.jpg)
![Western Blot: Adenine Nucleotide Translocator 2 Antibody [NBP2-57642] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/images2/Adenine-Nucleotide-Translocator-2-Antibody-Western-Blot-NBP2-57642-img0002.jpg)
![Immunohistochemistry-Paraffin: Adenine Nucleotide Translocator 2 Antibody [NBP2-57642] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/Adenine-Nucleotide-Translocator-2-Antibody-Immunohistochemistry-Paraffin-NBP2-57642-img0004.jpg)
![Immunohistochemistry-Paraffin: Adenine Nucleotide Translocator 2 Antibody [NBP2-57642] - Staining of human cerebellum shows strong granular cytoplasmic positivity in Purkinje cells and cells in granular layer.](http://images.novusbio.com/images2/Adenine-Nucleotide-Translocator-2-Antibody-Immunohistochemistry-Paraffin-NBP2-57642-img0003.jpg)
![Western Blot: CD77 Synthase Antibody [NBP1-90824] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Negative control (vector only transfected HEK293T lysate)Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402588)](http://images.novusbio.com/images2/CD77-Synthase-Antibody-Western-Blot-NBP1-90824-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CD77 Synthase Antibody [NBP1-90824] - Staining of human cell line MCF7 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/images2/CD77-Synthase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90824-img0006.jpg)
![Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118] - 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor.](http://images.novusbio.com/images2/NMDAR1-Antibody-(R1JHL)-Western-Blot-NB300-118-img0001.jpg)
![Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118] - The blots show the examples of immunobands against NMDAR1 and beta-actin antibodies on the membrane, which was performed on the membrane protein prepared from the lumbar spinal cords of the offspring (P14) rats in different groups. Image collected and cropped by CiteAb from the following publication (http://www.jbiomedsci.com/content/18/1/64) licensed under a CC-BY licence.](http://images.novusbio.com/images2/NMDAR1 Antibody (R1JHL)-Western Blot-NB300-118-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - NR1 surface staining of mouse corical neruons. This image was submitted via customer Review.](http://images.novusbio.com/images2/NMDAR1-Antibody-(R1JHL)-Immunocytochemistry-Immunofluorescence-NB300-118-img0003.jpg)
![Western Blot: Exosome component 10 Antibody [NBP1-32870] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EXOSC10 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/images2/Exosome-component-10-Antibody-Western-Blot-NBP1-32870-img0015.jpg)
![Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-32870] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/images2/Exosome-component-10-Antibody-Immunohistochemistry-Paraffin-NBP1-32870-img0010.jpg)
![Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of EXOSC 10 protein by 2. 5 ug of EXOSC 10 antibody 7. 5% SDS-PAGE gel.](http://images.novusbio.com/images2/Exosome-component-10-Antibody-Western-Blot-NBP1-32870-img0011.jpg)
![Western Blot: Perforin Antibody (CB5.4) [NBP1-97512] - Detection of perforin in the mouse T cell clone B6.1. (lane 1). Absence of perforin in the mouse fibroblast cell line NIH.3T3 (lane 2). The protein migrates as a 66 kDa species. Method: Cell extracts from the T cell clone B6.1 (2x10^6) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.](http://images.novusbio.com/images/Perforin-Antibody-(CB5.4)-Western-Blot-NBP1-97512-img0001.jpg)
![Western Blot: B3GALNT1 Antibody [NBP2-92677] - Analysis of extracts of various cell lines, using B3GALNT1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/images2/B3GALNT1 Antibody-Western Blot-NBP2-92677-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: B3GALNT1 Antibody [NBP2-92677] - Analysis of U2OS cells using B3GALNT1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/B3GALNT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92677-img0001.jpg)
![SDS-Page: Mouse IgG1 Isotype Control [NBP1-97005] - Lane 1:, Non-reduced. M: Opal Pre-stained Ladder. Lane 2:, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](http://images.novusbio.com/images2/Mouse-IgG1-Isotype-Control-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control [NBP1-97005] - Lane 1:, Non-reduced. M: Opal Pre-stained Ladder. Lane 2:, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")