![Western Blot: CDCA7L Antibody (OTI3B5) [NBP2-46198] - Detection of CDCA7L by western blot. Samples: Whole cell lysate from prostate cancer; 40 ug. Antibodies:CDCA7L Antibody [NBP2-46198] at dilutions 1:500, 1:1000, 1:2000. Detection: Chemiluminescence with exposure of 5 seconds. Image from verified customer review.](http://images.novusbio.com/fullsize2/CDCA7L-Antibody-(OTI3B5)-Western-Blot-NBP2-46198-img0010.jpg "Western Blot: CDCA7L Antibody (OTI3B5) [NBP2-46198] - Detection of CDCA7L by western blot. Samples: Whole cell lysate from prostate cancer; 40 ug. Antibodies:CDCA7L Antibody [NBP2-46198] at dilutions 1:500, 1:1000, 1:2000. Detection: Chemiluminescence with exposure of 5 seconds. Image from verified customer review.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | OTI3B5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein fragment corresponding to amino acids 1-337 of human CDCA7L (NP_061189) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CDCA7L |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 52 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
![IL-6 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "IL-6 [Unconjugated]")
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Greisha Lee Ortiz-Hernandez |
WB | Human | 06/05/2018 |
Summary
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Secondary Antibodies |
Isotype Controls |
Diseases for CDCA7L Antibody (NBP2-46198)Discover more about diseases related to CDCA7L Antibody (NBP2-46198).
| Pathways for CDCA7L Antibody (NBP2-46198)View related products by pathway.
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| Greisha Lee Ortiz-Hernandez 06/05/2018 |
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| Application: | WB | |
| Species: | Human |
| Gene Symbol | CDCA7L |
![Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/CDCA7L-Antibody-(3B5)-Immunohistochemistry-NBP2-46198-img0007.jpg "Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Western Blot: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDCA7L.](http://images.novusbio.com/fullsize2/CDCA7L Antibody (3B5)-Western Blot-NBP2-46198-img0008.jpg "Western Blot: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDCA7L.")
![Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/CDCA7L-Antibody-(3B5)-Immunohistochemistry-NBP2-46198-img0001.jpg "Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/CDCA7L-Antibody-(3B5)-Immunohistochemistry-NBP2-46198-img0002.jpg "Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/CDCA7L-Antibody-(3B5)-Immunohistochemistry-NBP2-46198-img0003.jpg "Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/CDCA7L-Antibody-(3B5)-Immunohistochemistry-NBP2-46198-img0004.jpg "Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/CDCA7L-Antibody-(3B5)-Immunohistochemistry-NBP2-46198-img0005.jpg "Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/CDCA7L-Antibody-(3B5)-Immunohistochemistry-NBP2-46198-img0006.jpg "Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Western Blot: RRM2 Antibody [NBP1-31661] - Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize2/RRM2-Antibody-Western-Blot-NBP1-31661-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: RRM2 Antibody [NBP1-31661] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RRM2 protein stained by RRM2 antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize2/RRM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31661-img0011.jpg)
![Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Formalin fixed parafin embedded mouse tumor tissues were stained with RRM2 antibody at 1:50 dilution. This image was submitted via customer Review.](http://images.novusbio.com/fullsize2/RRM2 Antibody-Immunohistochemistry-Paraffin-NBP1-31661-img0012.jpg)
![Western Blot: CD82/Kai-1 Antibody [NBP1-76775] - A549 cell lysate with CD82 antibody at (A) 0.5 and (B) 1 ug/ml.](http://images.novusbio.com/fullsize2/CD82-Kai-1-Antibody-Western-Blot-NBP1-76775-img0007.jpg)
![Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody [NBP1-76775] - Human colon tissue with KAI1 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize2/CD82-Kai-1-Antibody-Immunohistochemistry-Paraffin-NBP1-76775-img0005.jpg)
![Immunofluorescence: CD82/Kai-1 Antibody [NBP1-76775] - Human Colon cells with KAI1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize2/CD82-Kai-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76775-img0006.jpg)
![Immunohistochemistry-Frozen: CD1a Antibody (NA1/34-HLK) [NB100-64852] - Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD1a antibody followed by HISTAR detection system. Medium power](http://images.novusbio.com/fullsize2/CD1a-Antibody-(NA1-34-HLK)-Immunohistochemistry-Frozen-NB100-64852-img0007.jpg)
![Flow Cytometry: CD1a Antibody (NA1/34-HLK) [NB100-64852] - Analysis using the FITC conjugate of NB100-64852. Staining of MOLT 4 cells.](http://images.novusbio.com/fullsize2/CD1a-Antibody-(NA1-34-HLK)-Flow-Cytometry-NB100-64852-img0005.jpg)
![Immunohistochemistry-Frozen: CD1a Antibody (NA1/34-HLK) [NB100-64852] - mmunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD1a antibody followed by HISTAR detection system. Low power](http://images.novusbio.com/fullsize2/CD1a-Antibody-(NA1-34-HLK)-Immunohistochemistry-Frozen-NB100-64852-img0006.jpg)
![Western Blot: IP3R1 Antibody [NBP1-21398] - Whole cell lysate from HeLa cells. Affinity purified rabbit anti-IP3R1 antibody. IP3R1 was also immunoprecipitated by rabbit anti-IP3R1 antibody NBP1-21397.](http://images.novusbio.com/fullsize2/IP3R1-Antibody-Western-Blot-NBP1-21398-img0002.jpg)
![Immunohistochemistry: IP3R1 Antibody [NBP1-21398] - Sample: FFPE section of mouse cerebellum. Antibody: Affinity purified rabbit anti- IP3R1 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize2/IP3R1-Antibody-Immunohistochemistry-NBP1-21398-img0005.jpg)
![Immunohistochemistry: IP3R1 Antibody [NBP1-21398] - Sample: FFPE section of mouse cerebellum. Antibody: Affinity purified rabbit anti- IP3R1 used at a dilution of 1:100 (2ug/ml). Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated (A120-601D4](http://images.novusbio.com/fullsize2/IP3R1 Antibody-Immunohistochemistry-NBP1-21398-img0006.jpg)
![Western Blot: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of hnRNP C1+C2 on different lysates using anti-hnRNP C1+C2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: HepG2](http://images.novusbio.com/fullsize2/hnRNP-C1-+-C2-Antibody-(SN0652)-Western-Blot-NBP2-67656-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in B16-F1 (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/hnRNP C1 + C2 Antibody (SN0652)-Immunocytochemistry-Immunofluorescence-NBP2-67656-img0010.jpg)
![Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of paraffin-embedded mouse skin tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/hnRNP-C1-+-C2-Antibody-(SN0652)-Immunohistochemistry-Paraffin-NBP2-67656-img0008.jpg)
![Western Blot: PMEL17/SILV Antibody (10E5) [NBP1-47974] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PMEL17/SILV(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PMEL17/SILV.](http://images.novusbio.com/fullsize/PMEL17-SILV Antibody (10E5)-Western Blot-NBP1-47974-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PMEL17/SILV Antibody (10E5) [NBP1-47974] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PMEL17/SILV.](http://images.novusbio.com/fullsize/PMEL17-SILV Antibody (10E5)-Immunocytochemistry-Immunofluorescence-NBP1-47974-img0016.jpg)
![Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (10E5) [NBP1-47974] - Staining of paraffin-embedded prostate tissue using anti-PMEL17/SILV mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PMEL17-SILV Antibody (10E5)-Immunohistochemistry-Paraffin-NBP1-47974-img0015.jpg)
![Western Blot: CD1c/BDCA-1 Antibody (2F4) [NBP2-46123] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD1c.](http://images.novusbio.com/fullsize2/CD1c-BDCA-1-Antibody-(2F4)-Western-Blot-NBP2-46123-img0007.jpg)
![Immunohistochemistry: CD1c/BDCA-1 Antibody (2F4) [NBP2-46123] - Analysis of Human endometrium tissue.](http://images.novusbio.com/fullsize2/CD1c-BDCA-1-Antibody-(2F4)-Immunohistochemistry-NBP2-46123-img0006.jpg)
![Western Blot: CD1c/BDCA-1 Antibody (2F4) [NBP2-46123] - Analysis of Jurkat extracts (10ug).](http://images.novusbio.com/fullsize2/CD1c-BDCA-1 Antibody (2F4)-Western Blot-NBP2-46123-img0008.jpg)
![Western Blot: Adenine Nucleotide Translocator 2 Antibody [NBP2-57642] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize2/Adenine Nucleotide Translocator 2 Antibody-Western Blot-NBP2-57642-img0002.jpg)
![Immunohistochemistry-Paraffin: Adenine Nucleotide Translocator 2 Antibody [NBP2-57642] - Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize2/Adenine-Nucleotide-Translocator-2-Antibody-Immunohistochemistry-Paraffin-NBP2-57642-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD77 Synthase Antibody [NBP2-57614] - Staining of human cell line U-2 OS shows localization to nuclear bodies & mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/CD77 Synthase Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57614-img0001.jpg)
![Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118]](http://images.novusbio.com/fullsize2/NMDAR1-Antibody-(R1JHL)-Western-Blot-NB300-118-img0001.jpg)
![Immunocytochemistry: NMDAR1 Antibody (R1JHL) [NB300-118] - NR1 surface staining of mouse corical neruons. This image was submitted via customer Review.](http://images.novusbio.com/fullsize2/NMDAR1-Antibody-(R1JHL)-Immunocytochemistry-NB300-118-img0003.jpg)
![Immunocytochemistry: NMDAR1 Antibody (R1JHL) [NB300-118] - Mixed rat cortical cultures (21 DIV) were labelled with anti-NMDAR1 (1:200) and anti-mouse CF568 (1:250). The antibody labels small punctae along the neuronal processes. No distinct features such as spines are visible. This image was submitted via customer Review.](http://images.novusbio.com/fullsize2/NMDAR1 Antibody (R1JHL)-Immunocytochemistry-NB300-118-img0004.jpg)
![Western Blot: Exosome component 10 Antibody [NBP1-32870] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EXOSC10 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Exosome-component-10-Antibody-Western-Blot-NBP1-32870-img0015.jpg)
![Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-32870] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize2/Exosome-component-10-Antibody-Immunohistochemistry-Paraffin-NBP1-32870-img0010.jpg)
![Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of EXOSC 10 protein by 2. 5 ug of EXOSC 10 antibody 7. 5% SDS-PAGE gel.](http://images.novusbio.com/fullsize2/Exosome-component-10-Antibody-Western-Blot-NBP1-32870-img0011.jpg)
![Western Blot: Perforin Antibody (CB5.4) [NBP1-97512] - Detection of perforin in the mouse T cell clone B6.1. (lane 1). Absence of perforin in the mouse fibroblast cell line NIH.3T3 (lane 2). The protein migrates as a 66 kDa species. Method: Cell extracts from the T cell clone B6.1 (2x10^6) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.](http://images.novusbio.com/fullsize/Perforin-Antibody-(CB5.4)-Western-Blot-NBP1-97512-img0001.jpg)
![SDS-Page: Mouse IgG1 Isotype Control [NBP1-97005] - Lane 1:, Non-reduced. M: Opal Pre-stained Ladder. Lane 2:, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](http://images.novusbio.com/images2/Mouse-IgG1-Isotype-Control-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control [NBP1-97005] - Lane 1:, Non-reduced. M: Opal Pre-stained Ladder. Lane 2:, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
![SDS-Page: Recombinant Human CDCA7L Protein [H00055536-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/images/qc_test-H00055536-P01-1.jpg "SDS-Page: Recombinant Human CDCA7L Protein [H00055536-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")