![Western Blot: CDC7 Antibody (DCS-341) [NB120-10535] - Whole extract of A431 cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Cdc7 Kinase Clone: DCS-341 and developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.](http://images.novusbio.com/fullsize/CDC7-Antibody-DCS-341-Western-Blot-NB120-10535-img0001.jpg "Western Blot: CDC7 Antibody (DCS-341) [NB120-10535] - Whole extract of A431 cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Cdc7 Kinase Clone: DCS-341 and developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IP, MA |
| Clone | DCS-341 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 2.0 mg/ml |
| Description | Novus Biologicals Mouse CDC7 Antibody (DCS-341) - BSA Free (NB120-10535) is a monoclonal antibody validated for use in WB, ELISA, ICC/IF and IP. Anti-CDC7 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | full-length human Cdc7 kinase. |
| Localization | Nuclear |
| Specificity | Mouse Monoclonal to Cdc7 Kinase (DCS-341) |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CDC7 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. Western Blot: Use at a concentration of 0.4 ug/ml. Detects a band of approximately 55 kDa. Optimal dilutions/concentrations should be determined by the end user. |
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| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 10mM PBS (pH 7.4) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 2.0 mg/ml |
| Purity | Protein A or G purified |
| Publication using NB120-10535 | Applications | Species |
|---|---|---|
| Hubbi ME, Kshitiz, Gilkes DMet al. A nontranscriptional role for HIF-1alpha as a direct inhibitor of DNA replication. Sci Signal 2013-02-12 [PMID: 23405012] |
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
WB | Human | 10/04/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for CDC7 Antibody (NB120-10535)Find related products by research area.
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")