CDC7 Antibody (DCS-341)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP, MiAr
Clone
DCS-341
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
2.0 mg/ml

Order Details

CDC7 Antibody (DCS-341) Summary

Immunogen
full-length human Cdc7 kinase.
Localization
Nuclear
Specificity
Mouse Monoclonal to Cdc7 Kinase (DCS-341)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CDC7
Purity
Protein A or G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Microarray
Application Notes
ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. Western Blot: Use at a concentration of 0.4 ug/ml. Detects a band of approximately 55 kDa. Optimal dilutions/concentrations should be determined by the end user.
Positive Control
A-431 Lysate (NB820-59461)
Reviewed Applications
Read 1 Review rated 4
using
NB120-10535 in the following applications:

Publications
Read Publication using NB120-10535.

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
2.0 mg/ml
Purity
Protein A or G purified

Alternate Names for CDC7 Antibody (DCS-341)

  • CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1
  • CDC7 cell division cycle 7 (S. cerevisiae)
  • CDC7L1MGC117361
  • CDC7-related kinase
  • cell division cycle 7 (S. cerevisiae)
  • cell division cycle 7 homolog (S. cerevisiae)
  • cell division cycle 7-like protein 1
  • cell division cycle 7-related protein kinase
  • EC 2.7.11.1
  • HsCDC7
  • Hsk1
  • huCdc7
  • MGC126237
  • MGC126238

Background

DNA replication in eukaryotic cells is dependent on the phosphorylation of the pre-replicative complex (preRC) at the origin of replication. Two complexes of proteins mediate this event, the cyclin dependent kinase (CDK) complex, and the Cdc7 kinase-ASK complex. Human Cdc7 kinase consists of 574 amino acids with a molecular weight of 55 kDa. The activity of Cdc7 kinase oscillates during cell cycle. The major targets of Cdc7 kinase are proteins that belong to the MCM complex (mini chromosome maintenance proteins). Cdc7 kinase was also found to be important in meiosis, checkpoint responses, maintenance of chromosome structure, and repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CDC7 Antibody (NB120-10535)(1)

Review for CDC7 Antibody (NB120-10535) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB120-10535:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 10/04/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionSheep anti-mouse IgG, HRP
Secondary Concentration1:2000

Details

Detection NotesRegular Protocol

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CDC7 Antibody (NB120-10535) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for CDC7 Antibody (NB120-10535)

Discover related pathways, diseases and genes to CDC7 Antibody (NB120-10535). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for CDC7 Antibody (NB120-10535)

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Pathways for CDC7 Antibody (NB120-10535)

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Research Areas for CDC7 Antibody (NB120-10535)

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Blogs on CDC7

There are no specific blogs for CDC7, but you can read our latest blog posts.

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Contact Information

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Calculators

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Recent Reviews

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4
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1
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Anonymous
10/04/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol CDC7
Entrez