CD98 Antibody (590559) [Unconjugated]

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human CD98 Monoclonal Antibody (Catalog # MAB5920, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).

CD98 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human CD98 Monoclonal Antibody (Catalog # MAB5920) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 590559
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CD98 Antibody (590559) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human CD98
  Asp105-Gln529
  Accession # P08195
• Specificity: Detects human CD98 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC3A2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD98 Antibody (590559) [Unconjugated]

• 4F2
• 4F2hc
• 4T2HC
• antigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43,4F2HC
• CD98 antigen
• CD98 heavy chain
• CD98
• CD98HC
• CH98hc
• FRP-1
• heavy chain
• Lymphocyte activation antigen 4F2 large subunit
• MDU1antigen defined by monoclonal 4F2, heavy chain
• monoclonal 44D7
• NACAE4F2 cell-surface antigen heavy chain
• SLC3A2
• solute carrier family 3 (activators of dibasic and neutral amino acidtransport), member 2,4F2 heavy chain antigen

Background

CD98, also known as 4F2, is a widely expressed disulfide-linked heterodimer in the solute transporter family. It consists of an 85 kDa glycosylated type II transmembrane heavy chain and a 40‑50 kDa non-glycosylated light chain with 12 transmembrane segments. The heavy chain (SLC3A2) pairs with one of several light chains (SLC7A5, 6, 7, 8, 10, or 11) and is required for the cell surface expression and amino acid transport function of the light chains. CD98 also mediates integrin signaling, T cell costimulation, B cell proliferation, and viral fusion with cell membranes. Within the extracellular region, human CD98 heavy chain shares 73% and 71% aa sequence identity with mouse and rat CD98 heavy chain, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD98 Antibody (MAB5920)(1)

Publication using MAB5920

• S Gong, Y Li, W Su, Y Ding, J Lu, K Dong, S Hood, W Zhang, GC Terstappen Quantitative Algorithm-Based Paired Imaging Measurement for Antibody-Triggered Endocytosis in Cultured Cells SLAS Discov, 2018-03-05;0(0):2472555218761. 2018-03-05 [PMID: 29505735]

Bioinformatics

• Gene Symbol: SLC3A2
• Uniprot:
  • P08195()