CD90.1 Antibody (HIS51) - BSA Free

Flow Cytometry: CD90.1 Antibody (HIS51) [NBP2-00312] - Analysis using the Biotin conjugate of NBP2-00312. Staining of rat splenocytes with Anti-Mouse/Rat CD90.1 (Thy-1.1) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

• Reactivity: Mu, Rt
• Applications: Flow, ICC/IF, IHC
• Clone: HIS51
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

CD90.1 Antibody (HIS51) - BSA Free Summary

• Immunogen: The immunogen for this antibody is CD90.1 (Thy-1.1, Thy1.1).
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: THY1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD90.1 Antibody (HIS51) - BSA Free

• thy-1 membrane glycoprotein
• CD90
• Thy-1 cell surface antigen

Background

The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD90.1 Antibody (NBP2-00312)(2)

Publications using NBP2-00312

• Paiva GR. Celulas-tronco na neurorrafia latero-terminal: estudo experimental em ratos. Thesis. 2016-01-01 (IHC-P, Rat)
• Hayashi T, Sano K, Aburatani H et al. Initialization of epithelial cells by tumor cells in a metastatic microenvironment Oncogene 2020-02-03 [PMID: 32015485] (IF/IHC)

Bioinformatics

• Gene Symbol: THY1