CD90/Thy1 Antibody (G7)

Images

 
Immunocytochemistry/ Immunofluorescence: CD90/Thy1 Antibody (G7) [NBP1-28031] - Primary cortical neurons isolated from postnatal day 7 CD1 mice. Primary antibody incubation overnight at 1:500. Alexa Fluor secondary ...read more
Flow Cytometry: CD90/Thy1 Antibody (G7) [NBP1-28031] - Flow Cytometry: CD90/Thy1 Antibody (G7) [Biotin] [NBP1-28033] - BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD90-BIOT and Rat Anti-Mouse CD3 ...read more
Flow Cytometry: CD90/Thy1 Antibody (G7) [NBP1-28031] - Analysis using the Allophycocyanin conjugate of NBP1-28031. Multiple staining of BALB/c splenocytes.
Flow Cytometry: CD90/Thy1 Antibody (G7) [NBP1-28031] - Analysis using the Biotin conjugate of NBP1-28031. Multiple staining of BALB/c splenocytes.
Flow Cytometry: CD90/Thy1 Antibody (G7) [NBP1-28031] - Analysis using the FITC conjugate of NBP1-28031. Staining of 1 ug/1^6 BALB/c mesenteric lymph node cells with either rat IgG2c-FITC (as a negative control) or rat ...read more
Flow Cytometry: CD90/Thy1 Antibody (G7) [NBP1-28031] - Analysis using the PE conjugate of NBP1-28031. Multiple staining of BALB/c splenocytes.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P, In vitro, IP, Func
Clone
G7
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

View Available Conjugates

CD90/Thy1 Antibody (G7) Summary

Immunogen
Mouse T cell hybridoma C6/G8
Marker
Mesenchymal Cell Marker
Specificity
Mouse CD90 (Thy-1.1 & Thy-1.2 alloantigens/Thy-1 epitope region A); Mr 28-32 kDa
Isotype
IgG2c Kappa
Clonality
Monoclonal
Host
Rat
Gene
THY1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • In vitro assay
  • Functional Assay
Application Notes
Use in ICC/IF reported by customer review.
Control
CD90/Thy1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NBP1-28031 in the following applications:

Publications
Read Publications using
NBP1-28031 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD90/Thy1 Antibody (G7)

  • CD90 antigen
  • CD90
  • CDw90
  • FLJ33325
  • Thy-1 antigen
  • Thy-1 cell surface antigen
  • thy-1 membrane glycoprotein
  • Thy-1 T-cell antigen
  • Thy1

Background

CD90/Thy-1, a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily of cell surface receptors, consists of a single V-set domain. (1-4) It is expressed on thymocytes, peripheral T lymphocytes, some intraepithelial T lymphocytes and neurons of all mouse strains. (1,2) MAb G7 stimulates T-cell proliferation and IL-2 secretion, via signalling through the T-cell receptor/CD3 complex. (1,5-7) The MAb has also been reported to promote apoptosis of thymocytes and CTL clones,4,7 and to mediate adhesion of thymocytes to thymic stroma.8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD90/Thy1 Antibody (NBP1-28031)(11)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: In vitro.


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In vitro
(4)
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Mouse
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP1-28031 Applications Species
Hueber, AO, G Raposo, M Pierres, HT He. Thy-1 triggers mouse thymocyte apoptosis through a bcl-2-resistant mechanism J Exp Med 1994 Mar [PMID: 7906706]

Details:
This citation used the Biotin version of this antibody.
Gunter, K, T Malek, E Shevach. T cell-activating properties of an anti-Thy-1 monoclonal antibody, possible analogy to OKT3/Leu-4 J Exp Med 1984 Mar 1 [PMID: 6142077] (In vitro)

Details:
This citation used the Biotin version of this antibody.
In vitro
Tentori, L, DM Pardoll, JC Zunigaq et al. Proliferation and production of IL-2 and B cell stimulatory factor 1/IL-4 in early fetal thymocytes by activation through Thy-1 and CD3 J Immunol 1988 Feb [PMID: 2893823] (In vitro)

Details:
This citation used the Biotin version of this antibody.
In vitro
Sugiyama, E, A Cantagrel, T Reno et al. Selection of T cell receptor expression mutants through the functionally linked Ly-6A Cell Immunol 1990 Oct [PMID: 1976441] (In vitro)

Details:
This citation used the Biotin version of this antibody.
In vitro
Ucker, DS, J Meyers, PS Obermiller. Activation-driven T cell death II Quantitative differences alone distinguish stimuli triggering nontransformed T cell proliferation or death J Immunol 1992 Sep [PMID: 1380534]

Details:
This citation used the Biotin version of this antibody.
Sugiyama, E, A Cantagrel, T Reno et al. Selection of T cell receptor expression mutants through the functionally linked Ly-6A Cell Immunol Jun 12 1905 (In vitro)

Details:
This publication used the Biotin conjugated form of this antibody (Cat# NBP1-28033).
In vitro
Gunter KC, Malek TR, Shevach EM et al. T cell-activating properties of an anti-Thy-1 monoclonal antibody, possible analogy to OKT3/Leu-4 J Exp Med

Details:
This publication used the FITC conjugated form of this antibody (Cat# NBP1-28032) and the Biotin conjugated form of this antibody (Cat# NBP1-28033).
Hueber AO, Raposo G, Pierres M et al. Thy-1 triggers mouse thymocyte apoptosis through a bcl-2-resistant mechanism J Exp Med

Details:
This publication used the FITC conjugated form of this antibody (Cat# NBP1-28032) and the Biotin conjugated form of this antibody (Cat# NBP1-28033).
Ucker DS, Meyers J, Obermiller PS et al. Activation-driven T cell death II Quantitative differences alone distinguish stimuli triggering nontransformed T cell proliferation or death J Immunol

Details:
This publication used the FITC conjugated form of this antibody (Cat# NBP1-28032) and the Biotin conjugated form of this antibody (Cat# NBP1-28033).
Tentori L, Pardoll DM, Zuniga JC et al. Proliferation and production of IL-2 and B cell stimulatory factor 1/IL-4 in early fetal thymocytes by activation through Thy-1 and CD3 J Immunol

Details:
This publication used the FITC conjugated form of this antibody (Cat# NBP1-28032) and the Biotin conjugated form of this antibody (Cat# NBP1-28033).
Show All 11 Publications.

Review for CD90/Thy1 Antibody (NBP1-28031) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-28031:
Filter by Applications
ICC
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry CD90/Thy1 NBP1-28031
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reviewed by:
Kevin Thorburn
ICC Mouse 07/23/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedCortical neurons
SpeciesMouse
LotE6412-T889

Comments

CommentsCortices from postnatal day 7 CD1 mice were dissected and dissociated according to the methods of LC Foo - Cold Spring Harbor 2013. The cell suspension was sequentially incubated on petri dishes coated with antibodies targeting CD45, O4, Thy1/CD90 and Itgb5. Cells adhered to the CD90 dish were removed with trypsin and replated in a neurobasal-based media containing 2% B-27 plus. Cells were fixed with PFA for 10 min 36h after being plated. The cells were then washed 3 times with D-PBS -Sigma D8662- and blocked for 30 minutes in D-PBS containing 0.2 percent Triton X100 and 10 percent donkey serum. The cells were then incubated overnight at 4 degrees with the primary antibody at 1 in 500 dilution in D-PBS containing 0.2 percent triton, 2 percent donkey serum and 2 percent bovine serum albumin - heat shock fraction. The following day the cells were washed twice with D-PBS and then incubated with Alexa Fluor secondaries - 1 in 500 dilution in the same antibody buffer described above - from ThermoFisher for 30min at room temperature. Cells were then washed 3 times with D-PBS and imaged.

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ICC/IF Video Protocol

FAQs for CD90/Thy1 Antibody (NBP1-28031). (Showing 1 - 1 of 1 FAQs).

  1. We are interested in CD90 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • I would recommend one of theses <a href="http://www.novusbio.com/productsearch/CD90%252FThy1#fq=common_name%3A%22CD90%2FThy1%22&fq=species%3A%22Canine%22" target="_self">CD90 antibodies</a>, as these have all been validated for use with dog/canine samples.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin NBP1-28035
Biotin NBP1-28033
FITC NBP1-28032
PE NBP1-28034

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Bioinformatics Tool for CD90/Thy1 Antibody (NBP1-28031)

Discover related pathways, diseases and genes to CD90/Thy1 Antibody (NBP1-28031). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on CD90/Thy1.

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CD90 is a 25-35kD glycosylphosphatidylinositol (GPI)-linked glycoprotein receptor of the immunoglobulin (Ig) superfamily. It is found on murine T-cells, thymocytes, neuronal cells, granulocytic lineage-derived cells, hematopoietic stem cells, fibrobla...  Read full blog post.

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Recent Reviews

5
5
1
4
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2
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1
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Kevin Thorburn
07/23/2019
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol THY1
Entrez
Uniprot
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