Immunocytochemistry/ Immunofluorescence: CD90/Thy1 Antibody (G7) [NBP1-28031] - Primary cortical neurons isolated from postnatal day 7 CD1 mice. Primary antibody incubation overnight at 1:500. Alexa Fluor secondary ...read more
Flow Cytometry: CD90/Thy1 Antibody (G7) [NBP1-28031] - Flow Cytometry: CD90/Thy1 Antibody (G7) [Biotin] [NBP1-28033] - BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD90-BIOT and Rat Anti-Mouse CD3 ...read more
Flow Cytometry: CD90/Thy1 Antibody (G7) [NBP1-28031] - Analysis using the Allophycocyanin conjugate of NBP1-28031. Multiple staining of BALB/c splenocytes.
Flow Cytometry: CD90/Thy1 Antibody (G7) [NBP1-28031] - Analysis using the Biotin conjugate of NBP1-28031. Multiple staining of BALB/c splenocytes.
Flow Cytometry: CD90/Thy1 Antibody (G7) [NBP1-28031] - Analysis using the FITC conjugate of NBP1-28031. Staining of 1 ug/1^6 BALB/c mesenteric lymph node cells with either rat IgG2c-FITC (as a negative control) or rat ...read more
Flow Cytometry: CD90/Thy1 Antibody (G7) [NBP1-28031] - Analysis using the PE conjugate of NBP1-28031. Multiple staining of BALB/c splenocytes.
CD90/Thy-1, a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily of cell surface receptors, consists of a single V-set domain. (1-4) It is expressed on thymocytes, peripheral T lymphocytes, some intraepithelial T lymphocytes and neurons of all mouse strains. (1,2) MAb G7 stimulates T-cell proliferation and IL-2 secretion, via signalling through the T-cell receptor/CD3 complex. (1,5-7) The MAb has also been reported to promote apoptosis of thymocytes and CTL clones,4,7 and to mediate adhesion of thymocytes to thymic stroma.8
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Cortices from postnatal day 7 CD1 mice were dissected and dissociated according to the methods of LC Foo - Cold Spring Harbor 2013. The cell suspension was sequentially incubated on petri dishes coated with antibodies targeting CD45, O4, Thy1/CD90 and Itgb5. Cells adhered to the CD90 dish were removed with trypsin and replated in a neurobasal-based media containing 2% B-27 plus. Cells were fixed with PFA for 10 min 36h after being plated. The cells were then washed 3 times with D-PBS -Sigma D8662- and blocked for 30 minutes in D-PBS containing 0.2 percent Triton X100 and 10 percent donkey serum. The cells were then incubated overnight at 4 degrees with the primary antibody at 1 in 500 dilution in D-PBS containing 0.2 percent triton, 2 percent donkey serum and 2 percent bovine serum albumin - heat shock fraction. The following day the cells were washed twice with D-PBS and then incubated with Alexa Fluor secondaries - 1 in 500 dilution in the same antibody buffer described above - from ThermoFisher for 30min at room temperature. Cells were then washed 3 times with D-PBS and imaged.
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FAQs for CD90/Thy1 Antibody (NBP1-28031). (Showing 1 - 1 of 1 FAQs).
We are interested in CD90 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
I would recommend one of theses <a href="http://www.novusbio.com/productsearch/CD90%252FThy1#fq=common_name%3A%22CD90%2FThy1%22&fq=species%3A%22Canine%22" target="_self">CD90 antibodies</a>, as these have all been validated for use with dog/canine samples.
CD90 (Cluster of differentiation 90) CD90 is a 25-35kD glycosylphosphatidylinositol (GPI)-linked glycoprotein receptor of the immunoglobulin (Ig) superfamily. It is found on murine T-cells, thymocytes, neuronal cells, granulocytic lineage-derived cells, hematopoietic stem cells, fibrobla... Read full blog post.
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