CD90/Thy1 Antibody (5E10) - BSA Free

Flow Cytometry: CD90/Thy1 Antibody (5E10) [NBP1-43379] - Analysis using the PerCP/Cy5.5 conjugate of NBP1-43379. Staining of the HEL cell line with Mouse IgG1 kappa Isotype Control PerCP-Cy5.5 (open histogram) or Anti-Human CD90 (Thy-1) PerCP-Cy5.5 (filled histogram). Total viable cells were used for analysis.

Flow Cytometry: CD90/Thy1 Antibody (5E10) [NBP1-43379] - Staining of human erythroleukemia (HEL) cell line with 0.25 ug of Mouse IgG1 kappa Isotype Control Purified (NBP1-43319) (open histogram) or 0.25 ug of Anti-Human CD90 (Thy-1) Purified (filled histogram) followed by Anti-Mouse IgG FITC. Total viable cells were used for analysis.

• Reactivity: Hu
• Applications: WB, Flow, IHC, IP
• Clone: 5E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

CD90/Thy1 Antibody (5E10) - BSA Free Summary

• Additional Information: Clone 5E10 was used by HLDA to establish CD designation.
• Immunogen: The immunogen for this antibody was CD90.
• Marker: Mesenchymal Cell Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: THY1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry < = 0.5 ug/10^6 cells in 100 ul
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: For Flow Cytometry: Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD90/Thy1 Antibody (5E10) - BSA Free

• CD90 antigen
• CD90
• CDw90
• FLJ33325
• Thy-1 antigen
• Thy-1 cell surface antigen
• thy-1 membrane glycoprotein
• Thy-1 T-cell antigen
• Thy1

Background

The 5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD90/Thy1 Antibody (NBP1-43379)(3)

Publications using NBP1-43379

• Hung JT, Liao JH, Lin YC Et al. Immunopathogenic role of TH1 cells in autoimmune diabetes: evidence from a T1 and T2 doubly transgenic non-obese diabetic mouse model. J Autoimmun 2005-11-03 [PMID: 16263243]
• Craig W, Kay R, Cutler RL, Lansdorp PM. Expression of Thy-1 on human hematopoietic progenitor cells. J Exp Med;177(5):1331-42. 1993-05-01 [PMID: 7683034] (WB, FLOW, IP)
• Mayani H, Lansdorp PM. Thy-1 expression is linked to functional properties of primitive hematopoietic progenitor cells from human umbilical cord blood. Blood;83(9):2410-7. 1994-05-01 [PMID: 7513197] (FLOW)

FAQs for CD90/Thy1 Antibody (NBP1-43379). (Showing 1 - 1 of 1 FAQs).

1. We are interested in CD90 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
   • I would recommend one of theses <a href="http://www.novusbio.com/productsearch/CD90%252FThy1#fq=common_name%3A%22CD90%2FThy1%22&fq=species%3A%22Canine%22" target="_self">CD90 antibodies</a>, as these have all been validated for use with dog/canine samples.

Bioinformatics

• Gene Symbol: THY1