CD9 Antibody (EM-04) - BSA Free

Western Blot: CD9 Antibody (EM-04) [NBP1-44876] - Detection of CD9 in bone marrow-derived mast cell (BMMC) lysates by rat monoclonal EM-04 antibody.

Flow Cytometry: CD9 Antibody (EM-04) [NBP1-44876] - Surface staining of CD9 in murine peritoneal fluid cells with anti-CD9 (EM-04) APC.

Flow Cytometry: CD9 Antibody (EM-04) [NBP1-44876] - Surface staining of CD9 in murine peritoneal fluid cells with anti-CD9 (EM-04) PE

Flow Cytometry: CD9 Antibody (EM-04) [NBP1-44876] - Surface staining of CD9 in murine peritoneal fluid cells with anti-CD9 (EM-04) purified, DAR-APC.

• Reactivity: Mu
• Applications: WB, Flow, ICC/IF, IP, CyTOF-ready
• Clone: EM-04
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CD9 Antibody (EM-04) - BSA Free Summary

• Description: Novus Biologicals Rat CD9 Antibody (EM-04) - BSA Free (NBP1-44876) is a monoclonal antibody validated for use in WB, Flow, ICC/IF and IP. Anti-CD9 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Permeabilized murine bone marrow-derived mast cells (BMMC).
• Specificity: The rat monoclonal antibody EM-04 recognizes CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: CD9
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 4 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:50
  • Western Blot 1 ug/ml
• Application Notes: Western Blotting: non-reducing conditions. This antibody is CyTOF ready.
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for CD9 Antibody (EM-04) - BSA Free

• BA2
• BA-2/p24 antigen
• BTCC-1
• CD9 antigen
• CD9 molecule
• CD9
• Cell growth-inhibiting gene 2 protein
• DRAP-27
• Leukocyte antigen MIC3
• MIC3
• motility related protein-1,5H9 antigen
• Motility-related protein
• MRP-1FLJ99568
• p24
• tetraspanin-29
• TSPAN29
• TSPAN-29
• TSPAN29MIC3CD9 antigen (p24)

Background

This antibody reacts with mouse CD9, a 24 kDa member of the transmembrane 4 superfamily. This family is characterized by the presence of four hydrophobic domains spanning the cell membrane and short N- and C-terminal cytoplasmic domains. CD9 is expressed by several cell types including monocytes, macrophages, platelets, early B cells, activated B and T cells, dendritic cells, eosinophils, basophils, endothelial cells, myoblasts and neuroblasts. On T cells, CD9 functions as a co-stimulatory molecule on na've T cells. Furthermore, CD9 is expressed in oocytes, and CD9-deficiency results in sterility caused by defective gamete fusion. In mouse macrophages, CD9 functionally associates with FcgammaRs to modify signals for phagocytosis and inflammatory responses. In mouse B cells, it was discovered that CD9 is a marker for marginal zone B cells, B1 cells, and plasma cells. In dendritic cells, recently it was demonstrated that CD9 facilitates the association of heterologous MHC II molecules. The level of CD9 expression is subject to donor variability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD9 Antibody (NBP1-44876)(2)

Publications using NBP1-44876

• Knuth CM, Barayan D, Lee JH et Al. Subcutaneous white adipose tissue independently regulates burn-induced hypermetabolism via immune-adipose crosstalk Cell Rep 2024-01-31 [PMID: 38117653]
• Lee J, Ealey K, Patel y et al. Characterization of adipose depot-specific stromal cell populations by single cell mass cytometry iScience 2022-03-01 [PMID: 35434565]

Bioinformatics

• Gene Symbol: CD9