CD9 Antibody (EM-04)

Images

 
Western Blot: CD9 Antibody (EM-04) [NBP1-44876] - Detection of CD9 in bone marrow-derived mast cell (BMMC) lysates by rat monoclonal EM-04 antibody.
Flow Cytometry: CD9 Antibody (EM-04) [NBP1-44876] - Surface staining of CD9 in murine peritoneal fluid cells with anti-CD9 (EM-04) APC.
Flow Cytometry: CD9 Antibody (EM-04) [NBP1-44876] - Surface staining of CD9 in murine peritoneal fluid cells with anti-CD9 (EM-04) PE
Flow Cytometry: CD9 Antibody (EM-04) [NBP1-44876] - Surface staining of CD9 in murine peritoneal fluid cells with anti-CD9 (EM-04) purified, DAR-APC.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, ICC/IF, IP, CyTOF-ready
Clone
EM-04
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1 mg/ml

CD9 Antibody (EM-04) Summary

Immunogen
Permeabilized murine bone marrow-derived mast cells (BMMC).
Specificity
The rat monoclonal antibody EM-04 recognizes CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
CD9
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Flow Cytometry 4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:50
  • CyTOF-ready
Application Notes
Western Blotting: non-reducing conditions

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for CD9 Antibody (EM-04)

  • BA2
  • BA-2/p24 antigen
  • BTCC-1
  • CD9 antigen
  • CD9 molecule
  • CD9
  • Cell growth-inhibiting gene 2 protein
  • DRAP-27
  • Leukocyte antigen MIC3
  • MIC3
  • motility related protein-1,5H9 antigen
  • Motility-related protein
  • MRP-1FLJ99568
  • p24
  • tetraspanin-29
  • TSPAN29
  • TSPAN-29
  • TSPAN29MIC3CD9 antigen (p24)

Background

This antibody reacts with mouse CD9, a 24 kDa member of the transmembrane 4 superfamily. This family is characterized by the presence of four hydrophobic domains spanning the cell membrane and short N- and C-terminal cytoplasmic domains. CD9 is expressed by several cell types including monocytes, macrophages, platelets, early B cells, activated B and T cells, dendritic cells, eosinophils, basophils, endothelial cells, myoblasts and neuroblasts. On T cells, CD9 functions as a co-stimulatory molecule on na've T cells. Furthermore, CD9 is expressed in oocytes, and CD9-deficiency results in sterility caused by defective gamete fusion. In mouse macrophages, CD9 functionally associates with FcgammaRs to modify signals for phagocytosis and inflammatory responses. In mouse B cells, it was discovered that CD9 is a marker for marginal zone B cells, B1 cells, and plasma cells. In dendritic cells, recently it was demonstrated that CD9 facilitates the association of heterologous MHC II molecules. The level of CD9 expression is subject to donor variability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CD9
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